An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus
Abstract Background Anthocyanins are metabolites of phenylpropanoid pathway, and involves in diverse processes of plant development and adaptation, which are regulated by the MYB-bHLH-WD40 (MBW) protein complexes. Many R2R3-MYB activators have been well characterized, but the MYB repressors in antho...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-022-02227-6 |
| _version_ | 1811207602398298112 |
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| author | Tao Xie Xiongyun Zan Xin Chen Haotian Zhu Hao Rong Youping Wang Jinjin Jiang |
| author_facet | Tao Xie Xiongyun Zan Xin Chen Haotian Zhu Hao Rong Youping Wang Jinjin Jiang |
| author_sort | Tao Xie |
| collection | DOAJ |
| description | Abstract Background Anthocyanins are metabolites of phenylpropanoid pathway, and involves in diverse processes of plant development and adaptation, which are regulated by the MYB-bHLH-WD40 (MBW) protein complexes. Many R2R3-MYB activators have been well characterized, but the MYB repressors in anthocyanin biosynthesis were recognized recently, which are also important in modulating phenylpropanoid metabolism in plants. The regulatory mechanism of anthocyanin biosynthesis in oil crop Brassica napus remains to be revealed. Results In this study, we identified an anthocyanin repressor BnCPC in B. napus. BnCPC encoded a typical R3-MYB protein containing a conserved [D/E]Lx2[R/K]x3Lx6Lx3R motif for interaction with bHLH proteins. Overexpression of BnCPC in B. napus inhibited anthocyanin accumulation, especially under anthocyanin inducible conditions. Protein–protein interaction and dual-luciferase assays confirmed that BnCPC could compete with BnPAP1 to interact with bHLHs (BnTT8 and BnEGL3), and repress the expression of anthocyanin biosynthetic genes (e.g., BnDFR) that activated by MBW complexes. Moreover, we found BnCPC inhibited the MBW complex-induced BnCPC activity. Conclusions Overall, this research demonstrated that BnCPC repressed anthocyanin biosynthesis by affecting the formation of MBW complex, and formed a feedback loop to regulate anthocyanin accumulation in B. napus. |
| first_indexed | 2024-04-12T04:07:17Z |
| format | Article |
| id | doaj.art-4518feb0257049d79ae500324380861e |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-12T04:07:17Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-4518feb0257049d79ae500324380861e2022-12-22T03:48:35ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542022-11-0115111610.1186/s13068-022-02227-6An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napusTao Xie0Xiongyun Zan1Xin Chen2Haotian Zhu3Hao Rong4Youping Wang5Jinjin Jiang6Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversitySchool of Biological and Food Engineering, Suzhou UniversityJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityAbstract Background Anthocyanins are metabolites of phenylpropanoid pathway, and involves in diverse processes of plant development and adaptation, which are regulated by the MYB-bHLH-WD40 (MBW) protein complexes. Many R2R3-MYB activators have been well characterized, but the MYB repressors in anthocyanin biosynthesis were recognized recently, which are also important in modulating phenylpropanoid metabolism in plants. The regulatory mechanism of anthocyanin biosynthesis in oil crop Brassica napus remains to be revealed. Results In this study, we identified an anthocyanin repressor BnCPC in B. napus. BnCPC encoded a typical R3-MYB protein containing a conserved [D/E]Lx2[R/K]x3Lx6Lx3R motif for interaction with bHLH proteins. Overexpression of BnCPC in B. napus inhibited anthocyanin accumulation, especially under anthocyanin inducible conditions. Protein–protein interaction and dual-luciferase assays confirmed that BnCPC could compete with BnPAP1 to interact with bHLHs (BnTT8 and BnEGL3), and repress the expression of anthocyanin biosynthetic genes (e.g., BnDFR) that activated by MBW complexes. Moreover, we found BnCPC inhibited the MBW complex-induced BnCPC activity. Conclusions Overall, this research demonstrated that BnCPC repressed anthocyanin biosynthesis by affecting the formation of MBW complex, and formed a feedback loop to regulate anthocyanin accumulation in B. napus.https://doi.org/10.1186/s13068-022-02227-6Brassica napusAnthocyaninBnCPCMBW complexRepressorBnDFR |
| spellingShingle | Tao Xie Xiongyun Zan Xin Chen Haotian Zhu Hao Rong Youping Wang Jinjin Jiang An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus Biotechnology for Biofuels and Bioproducts Brassica napus Anthocyanin BnCPC MBW complex Repressor BnDFR |
| title | An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus |
| title_full | An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus |
| title_fullStr | An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus |
| title_full_unstemmed | An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus |
| title_short | An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus |
| title_sort | r3 myb repressor bncpc forms a feedback regulation with mbw complex to modulate anthocyanin biosynthesis in brassica napus |
| topic | Brassica napus Anthocyanin BnCPC MBW complex Repressor BnDFR |
| url | https://doi.org/10.1186/s13068-022-02227-6 |
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