Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam
ABSTRACT: This study determined the extraction conditions, chemical composition, and antimicrobial activities of the essential oils of Citrus nobilis. The results illustrated that soaking in the 9% (w/v) NaCl solution for 2 h with a sample and solvent ratio of 1:3 (w/v) and a subsequent extraction t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2023-11-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000500401&tlng=en |
| _version_ | 1797628772026941440 |
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| author | Ly Thi Thuy Duyen Khong Hoang Thang Luu Minh Chau Tran Thi Giang Nguyen Ngoc Thanh Bui Hoang Dang Long Huynh Xuan Phong |
| author_facet | Ly Thi Thuy Duyen Khong Hoang Thang Luu Minh Chau Tran Thi Giang Nguyen Ngoc Thanh Bui Hoang Dang Long Huynh Xuan Phong |
| author_sort | Ly Thi Thuy Duyen |
| collection | DOAJ |
| description | ABSTRACT: This study determined the extraction conditions, chemical composition, and antimicrobial activities of the essential oils of Citrus nobilis. The results illustrated that soaking in the 9% (w/v) NaCl solution for 2 h with a sample and solvent ratio of 1:3 (w/v) and a subsequent extraction time of 45 min yielded the highest extraction efficiency, reaching 3.66% (w/w). The main chemical components of the essential oils were limonene (90.42%), β-myrcene (4.7%), and α-pinene (1.22%). The minimum inhibitory concentration (MIC) at the density of 5x104, 5x105, and 5x106 cells/mL of Escherichia coli were 100, 125, and 125 mg/mL, respectively; for Staphylococcus aureus were 75, 100, and 125 mg/mL, respectively; and for Bacillus cereus were 50, 75, and 100 mg/mL, respectively. The minimum bactericidal concentration (MBC) at densities of 5x104, 5x105, 5x106 cells/mL of E. coli were 175, 225, 225 mg/mL, S. aureus were 150, 200, 225 mg/mL, and B. cereus were 125, 175, 200 mg/mL, respectively. The inhibitory activities of Aspergillus flavus of orange essential oils according to the agar dilution method at concentrations of 0.025-0.1%) on the 5th day were 8.84-30.61%. |
| first_indexed | 2024-03-11T10:44:48Z |
| format | Article |
| id | doaj.art-451d8d4c4c6c4d78bc6884ebcb17c898 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-03-11T10:44:48Z |
| publishDate | 2023-11-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-451d8d4c4c6c4d78bc6884ebcb17c8982023-11-14T07:44:15ZengUniversidade Federal de Santa MariaCiência Rural1678-45962023-11-0154510.1590/0103-8478cr20230240Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, VietnamLy Thi Thuy Duyenhttps://orcid.org/0009-0002-7628-3572Khong Hoang Thanghttps://orcid.org/0009-0005-5859-8791Luu Minh Chauhttps://orcid.org/0009-0002-8411-2562Tran Thi Gianghttps://orcid.org/0009-0000-0550-8979Nguyen Ngoc Thanhhttps://orcid.org/0000-0002-3079-1259Bui Hoang Dang Longhttps://orcid.org/0000-0003-0926-9033Huynh Xuan Phonghttps://orcid.org/0000-0003-0220-2979ABSTRACT: This study determined the extraction conditions, chemical composition, and antimicrobial activities of the essential oils of Citrus nobilis. The results illustrated that soaking in the 9% (w/v) NaCl solution for 2 h with a sample and solvent ratio of 1:3 (w/v) and a subsequent extraction time of 45 min yielded the highest extraction efficiency, reaching 3.66% (w/w). The main chemical components of the essential oils were limonene (90.42%), β-myrcene (4.7%), and α-pinene (1.22%). The minimum inhibitory concentration (MIC) at the density of 5x104, 5x105, and 5x106 cells/mL of Escherichia coli were 100, 125, and 125 mg/mL, respectively; for Staphylococcus aureus were 75, 100, and 125 mg/mL, respectively; and for Bacillus cereus were 50, 75, and 100 mg/mL, respectively. The minimum bactericidal concentration (MBC) at densities of 5x104, 5x105, 5x106 cells/mL of E. coli were 175, 225, 225 mg/mL, S. aureus were 150, 200, 225 mg/mL, and B. cereus were 125, 175, 200 mg/mL, respectively. The inhibitory activities of Aspergillus flavus of orange essential oils according to the agar dilution method at concentrations of 0.025-0.1%) on the 5th day were 8.84-30.61%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000500401&tlng=enantibacterialantifungalCitrusnobilisessential oilsextraction conditionsorange peel |
| spellingShingle | Ly Thi Thuy Duyen Khong Hoang Thang Luu Minh Chau Tran Thi Giang Nguyen Ngoc Thanh Bui Hoang Dang Long Huynh Xuan Phong Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam Ciência Rural antibacterial antifungal Citrusnobilis essential oils extraction conditions orange peel |
| title | Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam |
| title_full | Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam |
| title_fullStr | Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam |
| title_full_unstemmed | Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam |
| title_short | Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam |
| title_sort | extraction and evaluation of antimicrobial activities of essential oils from orange peel citrus nobilis grown in can tho city vietnam |
| topic | antibacterial antifungal Citrusnobilis essential oils extraction conditions orange peel |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000500401&tlng=en |
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