Three-dimensional super-resolution imaging for fluorescence emission difference microscopy
We propose a method theoretically to break the diffraction limit and to improve the resolution in all three dimensions for fluorescence emission difference microscopy. We produce two kinds of hollow focal spot by phase modulation. By incoherent superposition, these two kinds of focal spot yield a 3D...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2015-08-01
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| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/1.4915132 |
| _version_ | 1818525459692912640 |
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| author | Shangting You Cuifang Kuang Shuai Li Xu Liu Zhihua Ding |
| author_facet | Shangting You Cuifang Kuang Shuai Li Xu Liu Zhihua Ding |
| author_sort | Shangting You |
| collection | DOAJ |
| description | We propose a method theoretically to break the diffraction limit and to improve the resolution in all three dimensions for fluorescence emission difference microscopy. We produce two kinds of hollow focal spot by phase modulation. By incoherent superposition, these two kinds of focal spot yield a 3D hollow focal spot. The optimal proportion of these two kinds of spot is given in the paper. By employing 3D hollow focal spot, super-resolution image can be yielded by means of fluorescence emission difference microscopy, with resolution enhanced both laterally and axially. According to computation result, size of point spread function of three-dimensional super-resolution imaging is reduced by about 40% in all three spatial directions with respect to confocal imaging. |
| first_indexed | 2024-12-11T06:09:31Z |
| format | Article |
| id | doaj.art-451f2e2b52334e59ad34b6671677298a |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-12-11T06:09:31Z |
| publishDate | 2015-08-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-451f2e2b52334e59ad34b6671677298a2022-12-22T01:18:11ZengAIP Publishing LLCAIP Advances2158-32262015-08-0158084901084901-1010.1063/1.4915132002599ADVThree-dimensional super-resolution imaging for fluorescence emission difference microscopyShangting You0Cuifang Kuang1Shuai Li2Xu Liu3Zhihua Ding4State key laboratory of modern optical instrumentations, Zhejiang University, Hangzhou 310027, ChinaState key laboratory of modern optical instrumentations, Zhejiang University, Hangzhou 310027, ChinaState key laboratory of modern optical instrumentations, Zhejiang University, Hangzhou 310027, ChinaState key laboratory of modern optical instrumentations, Zhejiang University, Hangzhou 310027, ChinaState key laboratory of modern optical instrumentations, Zhejiang University, Hangzhou 310027, ChinaWe propose a method theoretically to break the diffraction limit and to improve the resolution in all three dimensions for fluorescence emission difference microscopy. We produce two kinds of hollow focal spot by phase modulation. By incoherent superposition, these two kinds of focal spot yield a 3D hollow focal spot. The optimal proportion of these two kinds of spot is given in the paper. By employing 3D hollow focal spot, super-resolution image can be yielded by means of fluorescence emission difference microscopy, with resolution enhanced both laterally and axially. According to computation result, size of point spread function of three-dimensional super-resolution imaging is reduced by about 40% in all three spatial directions with respect to confocal imaging.http://dx.doi.org/10.1063/1.4915132 |
| spellingShingle | Shangting You Cuifang Kuang Shuai Li Xu Liu Zhihua Ding Three-dimensional super-resolution imaging for fluorescence emission difference microscopy AIP Advances |
| title | Three-dimensional super-resolution imaging for fluorescence emission difference microscopy |
| title_full | Three-dimensional super-resolution imaging for fluorescence emission difference microscopy |
| title_fullStr | Three-dimensional super-resolution imaging for fluorescence emission difference microscopy |
| title_full_unstemmed | Three-dimensional super-resolution imaging for fluorescence emission difference microscopy |
| title_short | Three-dimensional super-resolution imaging for fluorescence emission difference microscopy |
| title_sort | three dimensional super resolution imaging for fluorescence emission difference microscopy |
| url | http://dx.doi.org/10.1063/1.4915132 |
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