Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy
Cancers have always been the most difficult to fight, the treatment of cancer is still not considered. Thus, exploring new anticancer drugs is still imminent. Traditional Chinese medicine has played an important role in the treatment of cancer. Polyphenol oxidase (PPO) extracted from <i>Edible...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/5/1515 |
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| author | Qinqin Yuan Huixia Guo Jiajie Ding Chan Jiao Yalei Qi Hajra Zafar Xueyun Ma Faisal Raza Jianqiu Han |
| author_facet | Qinqin Yuan Huixia Guo Jiajie Ding Chan Jiao Yalei Qi Hajra Zafar Xueyun Ma Faisal Raza Jianqiu Han |
| author_sort | Qinqin Yuan |
| collection | DOAJ |
| description | Cancers have always been the most difficult to fight, the treatment of cancer is still not considered. Thus, exploring new anticancer drugs is still imminent. Traditional Chinese medicine has played an important role in the treatment of cancer. Polyphenol oxidase (PPO) extracted from <i>Edible mushroom</i> has many related reports on its characteristics, but its role in cancer treatment is still unclear. This study aims to investigate the effects of PPO extracted from <i>Edible mushroom</i> on the proliferation, migration, invasion, and apoptosis of cancer cells in vitro and explore the therapeutic effects of PPO on tumors in vivo. A cell counting kit-8 (CCK8) assay was used to detect the effect of PPO on the proliferation of cancer cells. The effect of PPO on cancer cell migration ability was detected by scratch test. The effect of PPO on the invasion ability of cancer cells was detected by a transwell assay. The effect of PPO on the apoptosis of cancer cells was detected by flow cytometry. Female BALB/c mice (18–25 g, 6–8 weeks) were used for in vivo experiments. The experiments were divided into control group, model group, low-dose group (25 mg/kg), and high-dose group (50 mg/kg). In vitro, PPO extracted from <i>Edible mushroom</i> significantly inhibited the proliferation, migration, and invasion capability of breast cancer cell 4T1, lung cancer cell A549, and prostate cancer cell C4-2, and significantly promoted the apoptosis of 4T1, A549, and C4-2. In vivo experiments showed PPO inhibitory effect on tumor growth. Collectively, the edible fungus extract PPO could play an effective role in treating various cancers, and it may potentially be a promising agent for treating cancers. |
| first_indexed | 2024-03-09T20:29:59Z |
| format | Article |
| id | doaj.art-45211979aac54d0fa37252c51fb24a64 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:29:59Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-45211979aac54d0fa37252c51fb24a642023-11-23T23:25:31ZengMDPI AGMolecules1420-30492022-02-01275151510.3390/molecules27051515Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer TherapyQinqin Yuan0Huixia Guo1Jiajie Ding2Chan Jiao3Yalei Qi4Hajra Zafar5Xueyun Ma6Faisal Raza7Jianqiu Han8College of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, ChinaCollege of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, ChinaCollege of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaCollege of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, ChinaCancers have always been the most difficult to fight, the treatment of cancer is still not considered. Thus, exploring new anticancer drugs is still imminent. Traditional Chinese medicine has played an important role in the treatment of cancer. Polyphenol oxidase (PPO) extracted from <i>Edible mushroom</i> has many related reports on its characteristics, but its role in cancer treatment is still unclear. This study aims to investigate the effects of PPO extracted from <i>Edible mushroom</i> on the proliferation, migration, invasion, and apoptosis of cancer cells in vitro and explore the therapeutic effects of PPO on tumors in vivo. A cell counting kit-8 (CCK8) assay was used to detect the effect of PPO on the proliferation of cancer cells. The effect of PPO on cancer cell migration ability was detected by scratch test. The effect of PPO on the invasion ability of cancer cells was detected by a transwell assay. The effect of PPO on the apoptosis of cancer cells was detected by flow cytometry. Female BALB/c mice (18–25 g, 6–8 weeks) were used for in vivo experiments. The experiments were divided into control group, model group, low-dose group (25 mg/kg), and high-dose group (50 mg/kg). In vitro, PPO extracted from <i>Edible mushroom</i> significantly inhibited the proliferation, migration, and invasion capability of breast cancer cell 4T1, lung cancer cell A549, and prostate cancer cell C4-2, and significantly promoted the apoptosis of 4T1, A549, and C4-2. In vivo experiments showed PPO inhibitory effect on tumor growth. Collectively, the edible fungus extract PPO could play an effective role in treating various cancers, and it may potentially be a promising agent for treating cancers.https://www.mdpi.com/1420-3049/27/5/1515PPOcancer cellsproliferationmigrationinvasionapoptosis |
| spellingShingle | Qinqin Yuan Huixia Guo Jiajie Ding Chan Jiao Yalei Qi Hajra Zafar Xueyun Ma Faisal Raza Jianqiu Han Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy Molecules PPO cancer cells proliferation migration invasion apoptosis |
| title | Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy |
| title_full | Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy |
| title_fullStr | Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy |
| title_full_unstemmed | Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy |
| title_short | Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy |
| title_sort | polyphenol oxidase as a promising alternative therapeutic agent for cancer therapy |
| topic | PPO cancer cells proliferation migration invasion apoptosis |
| url | https://www.mdpi.com/1420-3049/27/5/1515 |
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