Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids
Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of in...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2022-06-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
| Subjects: | |
| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2622&vmd=Full |
| _version_ | 1828473417279471616 |
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| author | Kang Won Park Hyeon Yang Hayeon Wi Sun A Ock Poongyeon Lee In-Sul Hwang Bo Ram Lee |
| author_facet | Kang Won Park Hyeon Yang Hayeon Wi Sun A Ock Poongyeon Lee In-Sul Hwang Bo Ram Lee |
| author_sort | Kang Won Park |
| collection | DOAJ |
| description | Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology. |
| first_indexed | 2024-12-11T05:44:05Z |
| format | Article |
| id | doaj.art-4521201b47824a80b5e0b2fdc99a667f |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-11T05:44:05Z |
| publishDate | 2022-06-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-4521201b47824a80b5e0b2fdc99a667f2022-12-22T01:19:01ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632022-06-0137213614310.12750/JARB.37.2.136Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoidsKang Won Park0https://orcid.org/0000-0001-6487-1583Hyeon Yang1https://orcid.org/0000-0003-4162-4410Hayeon Wi2https://orcid.org/0000-0001-5008-4626Sun A Ock3https://orcid.org/0000-0002-0887-8200Poongyeon Lee4https://orcid.org/0000-0003-2447-0392In-Sul Hwang5https://orcid.org/0000-0003-2949-3075Bo Ram Lee6https://orcid.org/0000-0002-0537-6205Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, KoreaColumbia Center for Translational Immunology, Columbia University Irving Medical Center, Columbia University, New York 10032, USAAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, KoreaRecent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.https://www.e-jarb.org/journal/view.html?uid=2622&vmd=Fullapical-outbovineintestinal organoidwnt signaling pathway activation |
| spellingShingle | Kang Won Park Hyeon Yang Hayeon Wi Sun A Ock Poongyeon Lee In-Sul Hwang Bo Ram Lee Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids Journal of Animal Reproduction and Biotechnology apical-out bovine intestinal organoid wnt signaling pathway activation |
| title | Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids |
| title_full | Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids |
| title_fullStr | Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids |
| title_full_unstemmed | Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids |
| title_short | Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids |
| title_sort | effect of wnt signaling pathway activation on the efficient generation of bovine intestinal organoids |
| topic | apical-out bovine intestinal organoid wnt signaling pathway activation |
| url | https://www.e-jarb.org/journal/view.html?uid=2622&vmd=Full |
| work_keys_str_mv | AT kangwonpark effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids AT hyeonyang effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids AT hayeonwi effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids AT sunaock effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids AT poongyeonlee effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids AT insulhwang effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids AT boramlee effectofwntsignalingpathwayactivationontheefficientgenerationofbovineintestinalorganoids |