Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish
A fucose-rich fucoidan was purified from brown seaweed <i>Saccharina japonica</i>, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated H...
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MDPI AG
2020-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/6/316 |
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| author | Wanchun Su Lei Wang Xiaoting Fu Liying Ni Delin Duan Jiachao Xu Xin Gao |
| author_facet | Wanchun Su Lei Wang Xiaoting Fu Liying Ni Delin Duan Jiachao Xu Xin Gao |
| author_sort | Wanchun Su |
| collection | DOAJ |
| description | A fucose-rich fucoidan was purified from brown seaweed <i>Saccharina japonica</i>, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry. |
| first_indexed | 2024-03-10T19:10:01Z |
| format | Article |
| id | doaj.art-4522e7b0cfe741dca9c1b69126037be8 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T19:10:01Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4522e7b0cfe741dca9c1b69126037be82023-11-20T03:52:11ZengMDPI AGMarine Drugs1660-33972020-06-0118631610.3390/md18060316Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in ZebrafishWanchun Su0Lei Wang1Xiaoting Fu2Liying Ni3Delin Duan4Jiachao Xu5Xin Gao6College of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao 266003, ChinaDepartment of Marine Life Sciences, Jeju National University, Jeju Self-Governing Province 63243, KoreaCollege of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao 266003, ChinaCollege of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao 266003, ChinaState Key Lab of Seaweed Bioactive Substances, 1th Daxueyuan Road, Qingdao 266400, ChinaCollege of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao 266003, ChinaCollege of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao 266003, ChinaA fucose-rich fucoidan was purified from brown seaweed <i>Saccharina japonica</i>, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry.https://www.mdpi.com/1660-3397/18/6/316ROSPhaeophytacarbohydrateUVB irradiationHaCaT cellszebrafish |
| spellingShingle | Wanchun Su Lei Wang Xiaoting Fu Liying Ni Delin Duan Jiachao Xu Xin Gao Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish Marine Drugs ROS Phaeophyta carbohydrate UVB irradiation HaCaT cells zebrafish |
| title | Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish |
| title_full | Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish |
| title_fullStr | Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish |
| title_full_unstemmed | Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish |
| title_short | Protective Effect of a Fucose-Rich Fucoidan Isolated from <i>Saccharina japonica</i> against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish |
| title_sort | protective effect of a fucose rich fucoidan isolated from i saccharina japonica i against ultraviolet b induced photodamage in vitro in human keratinocytes and in vivo in zebrafish |
| topic | ROS Phaeophyta carbohydrate UVB irradiation HaCaT cells zebrafish |
| url | https://www.mdpi.com/1660-3397/18/6/316 |
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