Long-term implanted cOFM probe causes minimal tissue reaction in the brain.
This study investigated the histological tissue reaction to long-term implanted cerebral open flow microperfusion (cOFM) probes in the frontal lobe of the rat brain. Most probe-based cerebral fluid sampling techniques are limited in application time due to the formation of a glial scar that hinders...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3951198?pdf=render |
| _version_ | 1819135101583228928 |
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| author | Thomas Birngruber Arijit Ghosh Sonja Hochmeister Martin Asslaber Thomas Kroath Thomas R Pieber Frank Sinner |
| author_facet | Thomas Birngruber Arijit Ghosh Sonja Hochmeister Martin Asslaber Thomas Kroath Thomas R Pieber Frank Sinner |
| author_sort | Thomas Birngruber |
| collection | DOAJ |
| description | This study investigated the histological tissue reaction to long-term implanted cerebral open flow microperfusion (cOFM) probes in the frontal lobe of the rat brain. Most probe-based cerebral fluid sampling techniques are limited in application time due to the formation of a glial scar that hinders substance exchange between brain tissue and the probe. A glial scar not only functions as a diffusion barrier but also alters metabolism and signaling in extracellular brain fluid. cOFM is a recently developed probe-based technique to continuously sample extracellular brain fluid with an intact blood-brain barrier. After probe implantation, a 2 week healing period is needed for blood-brain barrier reestablishment. Therefore, cOFM probes need to stay in place and functional for at least 15 days after implantation to ensure functionality. Probe design and probe materials are optimized to evoke minimal tissue reaction even after a long implantation period. Qualitative and quantitative histological tissue analysis revealed no continuous glial scar formation around the cOFM probe 30 days after implantation and only a minor tissue reaction regardless of perfusion of the probe. |
| first_indexed | 2024-12-22T10:13:44Z |
| format | Article |
| id | doaj.art-45233177dd5c40499d90ecc04f78cb7b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T10:13:44Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-45233177dd5c40499d90ecc04f78cb7b2022-12-21T18:29:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9022110.1371/journal.pone.0090221Long-term implanted cOFM probe causes minimal tissue reaction in the brain.Thomas BirngruberArijit GhoshSonja HochmeisterMartin AsslaberThomas KroathThomas R PieberFrank SinnerThis study investigated the histological tissue reaction to long-term implanted cerebral open flow microperfusion (cOFM) probes in the frontal lobe of the rat brain. Most probe-based cerebral fluid sampling techniques are limited in application time due to the formation of a glial scar that hinders substance exchange between brain tissue and the probe. A glial scar not only functions as a diffusion barrier but also alters metabolism and signaling in extracellular brain fluid. cOFM is a recently developed probe-based technique to continuously sample extracellular brain fluid with an intact blood-brain barrier. After probe implantation, a 2 week healing period is needed for blood-brain barrier reestablishment. Therefore, cOFM probes need to stay in place and functional for at least 15 days after implantation to ensure functionality. Probe design and probe materials are optimized to evoke minimal tissue reaction even after a long implantation period. Qualitative and quantitative histological tissue analysis revealed no continuous glial scar formation around the cOFM probe 30 days after implantation and only a minor tissue reaction regardless of perfusion of the probe.http://europepmc.org/articles/PMC3951198?pdf=render |
| spellingShingle | Thomas Birngruber Arijit Ghosh Sonja Hochmeister Martin Asslaber Thomas Kroath Thomas R Pieber Frank Sinner Long-term implanted cOFM probe causes minimal tissue reaction in the brain. PLoS ONE |
| title | Long-term implanted cOFM probe causes minimal tissue reaction in the brain. |
| title_full | Long-term implanted cOFM probe causes minimal tissue reaction in the brain. |
| title_fullStr | Long-term implanted cOFM probe causes minimal tissue reaction in the brain. |
| title_full_unstemmed | Long-term implanted cOFM probe causes minimal tissue reaction in the brain. |
| title_short | Long-term implanted cOFM probe causes minimal tissue reaction in the brain. |
| title_sort | long term implanted cofm probe causes minimal tissue reaction in the brain |
| url | http://europepmc.org/articles/PMC3951198?pdf=render |
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