Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction
Context: Cardiovascular disease is a leading cause of mortality worldwide. Following acute myocardial infarction (AMI), cardiac cells release many cytokines and proteolytic enzymes that regulate the extracellular matrix (ECM), including matrix metalloproteinases (MMPs), tissue inhibitor of metallopr...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2024-05-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol12/jppres23.1937_12.3.586.pdf |
| _version_ | 1797265388872925184 |
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| author | Saskia Dyah Handari Mohammad Saifur Rahman Djanggan Sargowo Aulanni’am Aulanni’am Bayu Lestari Delvac Oceandy |
| author_facet | Saskia Dyah Handari Mohammad Saifur Rahman Djanggan Sargowo Aulanni’am Aulanni’am Bayu Lestari Delvac Oceandy |
| author_sort | Saskia Dyah Handari |
| collection | DOAJ |
| description | Context: Cardiovascular disease is a leading cause of mortality worldwide. Following acute myocardial infarction (AMI), cardiac cells release many cytokines and proteolytic enzymes that regulate the extracellular matrix (ECM), including matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and phosphorylated microtubule-associated protein 4 (Phospho/pMAP4).
Aims: To analyze the role of colchicine in ECM regulation post-ischemic in cell line culture towards MMPs, TIMPs, and pMAP4.
Methods: This in vitro experimental study was conducted on the 3T3 fibroblast cell line subjected to hypoxic conditions (CoCl2 treatment) and compared with 3T3 cells experiencing hypoxia/ischemic and colchicine treatment. MMP-1, TIMP-1, and pMAP4 expression were measured using flow cytometry. Data analysis was performed using One-Way ANOVA with a 95% confidence level.
Results: Treatment with colchicine in the 3T3 fibroblast cell culture regulated the extracellular matrix by increasing TIMP-1 expression and reducing MMP-1 and pMAP4 expression (p<0.0001) following ischemic conditions induced by CoCl2.
Conclusions: Colchicine effectively mitigates ECM degradation by enhancing TIMP-1 expression and inhibiting MMP-1 activity and pMAP4 expression post-ischemic conditions. |
| first_indexed | 2024-04-25T00:44:01Z |
| format | Article |
| id | doaj.art-45233ab9909d47bfb6de0c9bc138da92 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-25T00:44:01Z |
| publishDate | 2024-05-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-45233ab9909d47bfb6de0c9bc138da922024-03-12T06:43:03ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502024-05-0112358659310.56499/jppres23.1937_12.3.586Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarctionSaskia Dyah Handari0Mohammad Saifur Rahman1Djanggan Sargowo2Aulanni’am Aulanni’am3Bayu Lestari4Delvac Oceandy5Doctoral Program of Medical Science, Brawijaya University, Malang, East Java, Indonesia. Medical Faculty, Ciputra University Surabaya, Indonesia. Department of Cardiology and Cardiovascular Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.Department of Cardiology and Cardiovascular Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.Department of Chemistry, Faculty of Sciences, Universitas Brawijaya, Malang, Indonesia.Division of Cardiovascular Sciences, University of Manchester, United Kingdom.Division of Cardiovascular Sciences, University of Manchester, United Kingdom.Context: Cardiovascular disease is a leading cause of mortality worldwide. Following acute myocardial infarction (AMI), cardiac cells release many cytokines and proteolytic enzymes that regulate the extracellular matrix (ECM), including matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and phosphorylated microtubule-associated protein 4 (Phospho/pMAP4). Aims: To analyze the role of colchicine in ECM regulation post-ischemic in cell line culture towards MMPs, TIMPs, and pMAP4. Methods: This in vitro experimental study was conducted on the 3T3 fibroblast cell line subjected to hypoxic conditions (CoCl2 treatment) and compared with 3T3 cells experiencing hypoxia/ischemic and colchicine treatment. MMP-1, TIMP-1, and pMAP4 expression were measured using flow cytometry. Data analysis was performed using One-Way ANOVA with a 95% confidence level. Results: Treatment with colchicine in the 3T3 fibroblast cell culture regulated the extracellular matrix by increasing TIMP-1 expression and reducing MMP-1 and pMAP4 expression (p<0.0001) following ischemic conditions induced by CoCl2. Conclusions: Colchicine effectively mitigates ECM degradation by enhancing TIMP-1 expression and inhibiting MMP-1 activity and pMAP4 expression post-ischemic conditions.https://jppres.com/jppres/pdf/vol12/jppres23.1937_12.3.586.pdfcell linecolchicineextracellular matrixischemia |
| spellingShingle | Saskia Dyah Handari Mohammad Saifur Rahman Djanggan Sargowo Aulanni’am Aulanni’am Bayu Lestari Delvac Oceandy Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction Journal of Pharmacy & Pharmacognosy Research cell line colchicine extracellular matrix ischemia |
| title | Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction |
| title_full | Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction |
| title_fullStr | Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction |
| title_full_unstemmed | Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction |
| title_short | Colchicine modulates TIMP1, MMP1, and pMAP4 expression to enhance extracellular matrix degradation in 3T3 fibroblast-mediated myocardial infarction |
| title_sort | colchicine modulates timp1 mmp1 and pmap4 expression to enhance extracellular matrix degradation in 3t3 fibroblast mediated myocardial infarction |
| topic | cell line colchicine extracellular matrix ischemia |
| url | https://jppres.com/jppres/pdf/vol12/jppres23.1937_12.3.586.pdf |
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