Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq
Background: We aimed to determine species of liver fluke that predominately cause fascioliasis in sheep, goats, and cattle in the Sulaymaniyah Province, Iraq using the molecular technique of DNA sequencing and restriction fragment length polymorphism (RFLP). Methods: The samples were collected f...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-11-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3869 |
| _version_ | 1797394671074279424 |
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| author | Vilya Shwan Othman Abdullah Ahmed Hama Rostam Hama Zorab Abdolhossein Dalimi |
| author_facet | Vilya Shwan Othman Abdullah Ahmed Hama Rostam Hama Zorab Abdolhossein Dalimi |
| author_sort | Vilya Shwan Othman |
| collection | DOAJ |
| description |
Background: We aimed to determine species of liver fluke that predominately cause fascioliasis in sheep, goats, and cattle in the Sulaymaniyah Province, Iraq using the molecular technique of DNA sequencing and restriction fragment length polymorphism (RFLP).
Methods: The samples were collected from November 2021 to May 2022. The flukes were collected from infected livers of livestock at the slaughterhouse of Sulaymaniyah Governorate, Iraq. A total of 205 flukes were collected from 56 hosts, cattle (n=22), sheep (n=28), and goats (n=6). The specific primers for FCOX1 and 28S rDNA gene amplification were used. The PCR products were subjected to restriction fragment polymorphism (RFLP) assay using Hpy188III and Dra II restriction enzymes, besides DNA sequencing.
Results: The results showed the genetic polymorphisms among the flukes. Three patterns of RFLP were observed Fasciola hepatica, F. gigantica, and F. intermediate, where 28 of them displayed F. hepatica (sheep, n=14, goat, n=3 and cattle, n= 11), whereas 24 samples displayed the F. gigantica (sheep, n=12, goat, n=3 and cattle, n= 9), and only four samples belonged to F. intermediate (sheep n=3 and cattle, n=1). In addition, the result of the ribosomal DNA (28S rDNA) sequencing confirmed that the isolated flukes belonged to F. hepatica, F. gigantica and F. intermediate.
Conclusion: All three main species are present in the study area and F. hepatica predominated among the animal species in this area also, our results concluded that PCR-RFLP is a rapid and reliable method for liver fluke species identification.
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| first_indexed | 2024-03-09T00:23:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-03-09T00:23:09Z |
| publishDate | 2023-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-45239d2b288743d9a2ac6ea239ad59222023-12-12T04:54:23ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2023-11-0118410.18502/ijpa.v18i4.14264Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, IraqVilya Shwan Othman0Abdullah Ahmed Hama1Rostam Hama Zorab2Abdolhossein Dalimi3Medical Laboratory Department, College of Health Science and Medical Technology, Sulaimani Polytechnic Uni-versity, Kurdistan, Iraq1. Medical Laboratory Department, College of Health Science and Medical Technology, Sulaimani Polytechnic Uni-versity, Kurdistan, Iraq 2. MLS, College of Health Science, University of Human Development, Kurdistan IraqDirectorate of Veterinary in Sulaimani, Kurdistan, IraqDepartment of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background: We aimed to determine species of liver fluke that predominately cause fascioliasis in sheep, goats, and cattle in the Sulaymaniyah Province, Iraq using the molecular technique of DNA sequencing and restriction fragment length polymorphism (RFLP). Methods: The samples were collected from November 2021 to May 2022. The flukes were collected from infected livers of livestock at the slaughterhouse of Sulaymaniyah Governorate, Iraq. A total of 205 flukes were collected from 56 hosts, cattle (n=22), sheep (n=28), and goats (n=6). The specific primers for FCOX1 and 28S rDNA gene amplification were used. The PCR products were subjected to restriction fragment polymorphism (RFLP) assay using Hpy188III and Dra II restriction enzymes, besides DNA sequencing. Results: The results showed the genetic polymorphisms among the flukes. Three patterns of RFLP were observed Fasciola hepatica, F. gigantica, and F. intermediate, where 28 of them displayed F. hepatica (sheep, n=14, goat, n=3 and cattle, n= 11), whereas 24 samples displayed the F. gigantica (sheep, n=12, goat, n=3 and cattle, n= 9), and only four samples belonged to F. intermediate (sheep n=3 and cattle, n=1). In addition, the result of the ribosomal DNA (28S rDNA) sequencing confirmed that the isolated flukes belonged to F. hepatica, F. gigantica and F. intermediate. Conclusion: All three main species are present in the study area and F. hepatica predominated among the animal species in this area also, our results concluded that PCR-RFLP is a rapid and reliable method for liver fluke species identification. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3869Fasciola hepaticaFasciola giganticaRuminantsIraq |
| spellingShingle | Vilya Shwan Othman Abdullah Ahmed Hama Rostam Hama Zorab Abdolhossein Dalimi Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq Iranian Journal of Parasitology Fasciola hepatica Fasciola gigantica Ruminants Iraq |
| title | Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq |
| title_full | Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq |
| title_fullStr | Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq |
| title_full_unstemmed | Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq |
| title_short | Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq |
| title_sort | molecular characterization of liver fluke isolated from sheep goat and cattle in sulaymaniyah iraq |
| topic | Fasciola hepatica Fasciola gigantica Ruminants Iraq |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3869 |
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