TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
The aim of this work was to analyze the neuron morphology and morphometry of cervical, thoracic and lumbar areas of nonsymptomatic seropositive dogs’ spinal cord for toxoplasmosis. Twenty indefinite-breed adult dogs were used; ten dogs were healthy, with negative serology for toxoplasmosis, and wer...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2015-04-01
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| Series: | Ciência Animal Brasileira |
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| Online Access: | https://revistas.ufg.br/vet/article/view/18991 |
| _version_ | 1827914260326383616 |
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| author | Alessandra Cristina Francischini Carvalho Maria Rita Pacheco Silvana Martinez Baraldi-Artoni Annita Morais Girardi |
| author_facet | Alessandra Cristina Francischini Carvalho Maria Rita Pacheco Silvana Martinez Baraldi-Artoni Annita Morais Girardi |
| author_sort | Alessandra Cristina Francischini Carvalho |
| collection | DOAJ |
| description |
The aim of this work was to analyze the neuron morphology and morphometry of cervical, thoracic and lumbar areas of nonsymptomatic seropositive dogs’ spinal cord for toxoplasmosis. Twenty indefinite-breed adult dogs were used; ten dogs were healthy, with negative serology for toxoplasmosis, and were used as the control group (group 1), and ten dogs were nonsymptomatic but seropositive for toxoplasmosis (group 2). After the microtomy, with interval of 100 micrometers (µm), the histological 5-µm-thick cuts were dyed by hematoxylin-eosin and Masson's trichrome techniques. The glass slides were analyzed under light microscope to examine the neuron morphology. The parameters considered for the morphometric analysis were area, perimeter, maximum diameter, minimum diameter and shape factor of cytoplasm and nucleus of neuron. The results were statistically analyzed by Student’s t test at 5% probability level. The morphological characteristics between the two groups were similar and according to literature. The morphometric results showed that there were changes in neurons size and structure, and increase and loss of star shape were noticed in seropositive animals. The results suggest that the neurons of these dogs, yet nonsymptomatic, can have lost their conductor function.
Keywords: canine; central nervous system; histology; Toxoplasma gondii.
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| first_indexed | 2024-03-13T02:42:18Z |
| format | Article |
| id | doaj.art-4524e08f0dc2498a92645618b769e464 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-03-13T02:42:18Z |
| publishDate | 2015-04-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-4524e08f0dc2498a92645618b769e4642023-06-28T21:11:19ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912015-04-0116210.1590/cab.v16i2.18991TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGSAlessandra Cristina Francischini Carvalho0Maria Rita Pacheco1Silvana Martinez Baraldi-Artoni2Annita Morais Girardi3São Paulo State University - UNESP Campus JaboticabalSão Paulo State University - UNESP Campus JaboticabalSão Paulo State University - UNESP Campus JaboticabalSão Paulo State University - UNESP Campus Jaboticabal The aim of this work was to analyze the neuron morphology and morphometry of cervical, thoracic and lumbar areas of nonsymptomatic seropositive dogs’ spinal cord for toxoplasmosis. Twenty indefinite-breed adult dogs were used; ten dogs were healthy, with negative serology for toxoplasmosis, and were used as the control group (group 1), and ten dogs were nonsymptomatic but seropositive for toxoplasmosis (group 2). After the microtomy, with interval of 100 micrometers (µm), the histological 5-µm-thick cuts were dyed by hematoxylin-eosin and Masson's trichrome techniques. The glass slides were analyzed under light microscope to examine the neuron morphology. The parameters considered for the morphometric analysis were area, perimeter, maximum diameter, minimum diameter and shape factor of cytoplasm and nucleus of neuron. The results were statistically analyzed by Student’s t test at 5% probability level. The morphological characteristics between the two groups were similar and according to literature. The morphometric results showed that there were changes in neurons size and structure, and increase and loss of star shape were noticed in seropositive animals. The results suggest that the neurons of these dogs, yet nonsymptomatic, can have lost their conductor function. Keywords: canine; central nervous system; histology; Toxoplasma gondii. https://revistas.ufg.br/vet/article/view/18991CanineCentral nervous systemHistologyToxoplasma gondii |
| spellingShingle | Alessandra Cristina Francischini Carvalho Maria Rita Pacheco Silvana Martinez Baraldi-Artoni Annita Morais Girardi TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS Ciência Animal Brasileira Canine Central nervous system Histology Toxoplasma gondii |
| title | TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS |
| title_full | TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS |
| title_fullStr | TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS |
| title_full_unstemmed | TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS |
| title_short | TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS |
| title_sort | toxoplasmosis morphological and morphometric evaluation of spinal cord neurons from nonsymptomatic seropositive dogs |
| topic | Canine Central nervous system Histology Toxoplasma gondii |
| url | https://revistas.ufg.br/vet/article/view/18991 |
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