Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system
Atlantic sea scallops, Placopecten magellanicus, are the most economically important marine bivalves along the northeastern coast of North America. Wild harvest landings generate hundreds of millions of dollars, and wild-caught adults and juvenile spat are increasingly being cultured in aquaculture...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002326 |
| _version_ | 1797676611778117632 |
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| author | Suzanne L. Ishaq Sarah Hosler Adwoa Dankwa Phoebe Jekielek Damian C. Brady Erin Grey Hannah Haskell Rachel Lasley-Rasher Kyle Pepperman Jennifer Perry Brian Beal Timothy J. Bowden |
| author_facet | Suzanne L. Ishaq Sarah Hosler Adwoa Dankwa Phoebe Jekielek Damian C. Brady Erin Grey Hannah Haskell Rachel Lasley-Rasher Kyle Pepperman Jennifer Perry Brian Beal Timothy J. Bowden |
| author_sort | Suzanne L. Ishaq |
| collection | DOAJ |
| description | Atlantic sea scallops, Placopecten magellanicus, are the most economically important marine bivalves along the northeastern coast of North America. Wild harvest landings generate hundreds of millions of dollars, and wild-caught adults and juvenile spat are increasingly being cultured in aquaculture facilities and coastal farms. However, the last two weeks of the larval maturation phase in hatcheries are often plagued by large mortality events. Research into other scallop- and aquacultured-species point to bacterial infections or altered functionality of microbial communities which associate with the host. Despite intense filtering and sterilization of seawater, and changing tank water every 48 h, harmful microbes can still persist in biofilms and mortality is still high. There are no previous studies of the bacterial communities associated with the biofilms growing in scallop hatchery tanks, nor studies with wild or hatchery sea scallops. We characterized the bacterial communities in veliger-stage wild or hatchery larvae, and tank biofilms using the 16 S rRNA gene V3-V4 region sequenced on the Illumina MiSeq platform. Hatchery larvae had lower bacterial richness (number of bacteria taxa present) than the wild larvae and tank biofilms, and hatchery larvae had a similar bacterial community (which taxa were present) to both wild larvae and tank biofilms. Bacterial richness and community similarity between tank samples fluctuated over the trial in repeated patterns of rise and fall, which showed some correlation to lunar cycle that may be a proxy for the effects of spring tides and trends in seawater bacteria and phages which are propagated into hatchery tanks. These results along with future work, will inform hatcheries on methods that will increase larval survival in these facilities, for example, implementing additional filtering or avoiding seawater collection during spring tides, to reduce bacterial taxa of concern or promote a more diverse microbial community which would compete against pathogens. |
| first_indexed | 2024-03-11T22:31:44Z |
| format | Article |
| id | doaj.art-452751f2cd4d4acfa08776044102b258 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-11T22:31:44Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-452751f2cd4d4acfa08776044102b2582023-09-23T05:11:20ZengElsevierAquaculture Reports2352-51342023-10-0132101693Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery systemSuzanne L. Ishaq0Sarah Hosler1Adwoa Dankwa2Phoebe Jekielek3Damian C. Brady4Erin Grey5Hannah Haskell6Rachel Lasley-Rasher7Kyle Pepperman8Jennifer Perry9Brian Beal10Timothy J. Bowden11School of Food & Agriculture, University of Maine, Orono, ME 04469, United States; Corresponding author.School of Food & Agriculture, University of Maine, Orono, ME 04469, United StatesSchool of Food & Agriculture, University of Maine, Orono, ME 04469, United StatesEcology and Environmental Sciences, University of Maine, Orono, ME 04473, United StatesSchool of Marine Sciences, Darling Marine Center, University of Maine, Walpole, ME 04573, United StatesSchool of Biology and Ecology, University of Maine, Orono, ME 04469, United States; Maine Center for Genetics in the Environment, University of Maine, Orono, ME 04469, United StatesDepartment of Biological Sciences, University of Southern Maine, Portland, ME 04103, United StatesDepartment of Biological Sciences, University of Southern Maine, Portland, ME 04103, United StatesDowneast Institute, Beals, ME 04611, United StatesSchool of Food & Agriculture, University of Maine, Orono, ME 04469, United StatesDivision of Environmental & Biological Sciences, University of Maine at Machias, Machias, ME 04654, United StatesSchool of Food & Agriculture, University of Maine, Orono, ME 04469, United StatesAtlantic sea scallops, Placopecten magellanicus, are the most economically important marine bivalves along the northeastern coast of North America. Wild harvest landings generate hundreds of millions of dollars, and wild-caught adults and juvenile spat are increasingly being cultured in aquaculture facilities and coastal farms. However, the last two weeks of the larval maturation phase in hatcheries are often plagued by large mortality events. Research into other scallop- and aquacultured-species point to bacterial infections or altered functionality of microbial communities which associate with the host. Despite intense filtering and sterilization of seawater, and changing tank water every 48 h, harmful microbes can still persist in biofilms and mortality is still high. There are no previous studies of the bacterial communities associated with the biofilms growing in scallop hatchery tanks, nor studies with wild or hatchery sea scallops. We characterized the bacterial communities in veliger-stage wild or hatchery larvae, and tank biofilms using the 16 S rRNA gene V3-V4 region sequenced on the Illumina MiSeq platform. Hatchery larvae had lower bacterial richness (number of bacteria taxa present) than the wild larvae and tank biofilms, and hatchery larvae had a similar bacterial community (which taxa were present) to both wild larvae and tank biofilms. Bacterial richness and community similarity between tank samples fluctuated over the trial in repeated patterns of rise and fall, which showed some correlation to lunar cycle that may be a proxy for the effects of spring tides and trends in seawater bacteria and phages which are propagated into hatchery tanks. These results along with future work, will inform hatcheries on methods that will increase larval survival in these facilities, for example, implementing additional filtering or avoiding seawater collection during spring tides, to reduce bacterial taxa of concern or promote a more diverse microbial community which would compete against pathogens.http://www.sciencedirect.com/science/article/pii/S2352513423002326Aquaculture microbiomeHost associated microbiomeBacterial communityVibrio |
| spellingShingle | Suzanne L. Ishaq Sarah Hosler Adwoa Dankwa Phoebe Jekielek Damian C. Brady Erin Grey Hannah Haskell Rachel Lasley-Rasher Kyle Pepperman Jennifer Perry Brian Beal Timothy J. Bowden Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system Aquaculture Reports Aquaculture microbiome Host associated microbiome Bacterial community Vibrio |
| title | Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system |
| title_full | Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system |
| title_fullStr | Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system |
| title_full_unstemmed | Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system |
| title_short | Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system |
| title_sort | bacterial community trends associated with sea scallop placopecten magellanicus larvae in a hatchery system |
| topic | Aquaculture microbiome Host associated microbiome Bacterial community Vibrio |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002326 |
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