Effect of the HBV Capsid Assembly Inhibitor Bayer 41-4109 on the Intracellular Localization of EGFP-Core Fusion Proteins
Bayer 41-4109 is heteroarylpyrimidine (HAP) which has been identified as potent of HBV capsid assembly<br />inhibitor. The present study was to study effect of Bayer 41-4109 treatment on the intracellular localization of<br />EGFP-Core fusion proteins into HepG2 cells. Three recombinant...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2015-11-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/7775 |
| Summary: | Bayer 41-4109 is heteroarylpyrimidine (HAP) which has been identified as potent of HBV capsid assembly<br />inhibitor. The present study was to study effect of Bayer 41-4109 treatment on the intracellular localization of<br />EGFP-Core fusion proteins into HepG2 cells. Three recombinant plasmids of pEGFP-Core with single, double and<br />triple NLS of HBV core (EGFP-Core 1C, 2C and 3C ) and two recombinant plasmids with single and triple NLS of<br />SV-40 (EGFP-Core 1 and 3 SV-40) were used in this work. After transient transfected into HepG2 cells and treated<br />with Bayer 41-4109, the intracellular localization of expressed fusion proteins from all plasmid constructions were<br />determined and quantified under confocal laser microscope. Results shown that Bayer 41-4109 treatment in HepG2<br />cells inhibited the nuclear localization of EGFP-Core with single of triple HBV core NLS. As well as the constructions<br />of expressed fusion protein with single and triple SV-40 NLS (EGFP-Core 1 and 3 SV-40 NLS) showed<br />decreasing the nuclear localization after treated with Bayer 41-4109, even not as strong as EGFP-Core 1C and 3C<br />NLS. Bayer 41-4109 has been identified as a potent inhibitors of HBV replication which has multiple effects on HBV<br />capsid assembly. It may inhibit virus replication by inducing assembly inappropriately and by misdirecting<br />assembly decreasing the stability of normal capsids.<br />Keywords: HBV capsid, Bayer 41-4109, EGFP-Core fusion protein, HepG2 cell |
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| ISSN: | 0853-8654 2089-2241 |