Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells
Hechtian strands are thread-like structures in plasmolyzed plant cells that connect the cell wall to the plasma membrane. Although these strands were first observed more than 100 years ago, their physiological roles are largely unknown. Here, we used intracellular laser microdissection to examine th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/5/604 |
| _version_ | 1797568462354120704 |
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| author | Arata Yoneda Misato Ohtani Daisuke Katagiri Yoichiroh Hosokawa Taku Demura |
| author_facet | Arata Yoneda Misato Ohtani Daisuke Katagiri Yoichiroh Hosokawa Taku Demura |
| author_sort | Arata Yoneda |
| collection | DOAJ |
| description | Hechtian strands are thread-like structures in plasmolyzed plant cells that connect the cell wall to the plasma membrane. Although these strands were first observed more than 100 years ago, their physiological roles are largely unknown. Here, we used intracellular laser microdissection to examine the effects of disrupting Hechtian strands on plasmolyzed tobacco BY-2 cells. When we focused femtosecond laser pulses on Hechtian strands, targeted disruptions were induced, but no visible changes in cell morphology were detected. However, the calcofluor white signals from β-glucans was detected in plasmolyzed cells with disrupted Hechtian strands, whereas no signals were detected in untreated plasmolyzed cells. These results suggest that Hechtian strands play roles in sensing cell wall integrity. |
| first_indexed | 2024-03-10T19:56:17Z |
| format | Article |
| id | doaj.art-452db9a36c1f4e109b2a2dd42d38d148 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T19:56:17Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-452db9a36c1f4e109b2a2dd42d38d1482023-11-19T23:53:38ZengMDPI AGPlants2223-77472020-05-019560410.3390/plants9050604Hechtian Strands Transmit Cell Wall Integrity Signals in Plant CellsArata Yoneda0Misato Ohtani1Daisuke Katagiri2Yoichiroh Hosokawa3Taku Demura4Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, JapanHechtian strands are thread-like structures in plasmolyzed plant cells that connect the cell wall to the plasma membrane. Although these strands were first observed more than 100 years ago, their physiological roles are largely unknown. Here, we used intracellular laser microdissection to examine the effects of disrupting Hechtian strands on plasmolyzed tobacco BY-2 cells. When we focused femtosecond laser pulses on Hechtian strands, targeted disruptions were induced, but no visible changes in cell morphology were detected. However, the calcofluor white signals from β-glucans was detected in plasmolyzed cells with disrupted Hechtian strands, whereas no signals were detected in untreated plasmolyzed cells. These results suggest that Hechtian strands play roles in sensing cell wall integrity.https://www.mdpi.com/2223-7747/9/5/604Hechtian strandsplasma membranecell wallplasmolysisfemtosecond laser microdissectioncell wall regeneration |
| spellingShingle | Arata Yoneda Misato Ohtani Daisuke Katagiri Yoichiroh Hosokawa Taku Demura Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells Plants Hechtian strands plasma membrane cell wall plasmolysis femtosecond laser microdissection cell wall regeneration |
| title | Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells |
| title_full | Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells |
| title_fullStr | Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells |
| title_full_unstemmed | Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells |
| title_short | Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells |
| title_sort | hechtian strands transmit cell wall integrity signals in plant cells |
| topic | Hechtian strands plasma membrane cell wall plasmolysis femtosecond laser microdissection cell wall regeneration |
| url | https://www.mdpi.com/2223-7747/9/5/604 |
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