Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time
Maize is one of the most important food and feed crops in China due to the high demand for its use by both humans and animals. The Huang-Huai-Hai region is a major maize cultivation area in China, and the special cropping rotation system of wheat and maize requires a suitable reproductive period for...
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| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/8/2028 |
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| author | Chenhui Ma Kuntai Dang Qiankun Xie Javed Hussain Sahito Baiyu Yuan Jiong Wan Xiaoqian Qiu Jiawen Zhao Yanan Lin Shujun Meng Liqin Mu Dong Ding Huili Yang Yadong Xue Xiaoyang Chen Xuehai Zhang Jihua Tang |
| author_facet | Chenhui Ma Kuntai Dang Qiankun Xie Javed Hussain Sahito Baiyu Yuan Jiong Wan Xiaoqian Qiu Jiawen Zhao Yanan Lin Shujun Meng Liqin Mu Dong Ding Huili Yang Yadong Xue Xiaoyang Chen Xuehai Zhang Jihua Tang |
| author_sort | Chenhui Ma |
| collection | DOAJ |
| description | Maize is one of the most important food and feed crops in China due to the high demand for its use by both humans and animals. The Huang-Huai-Hai region is a major maize cultivation area in China, and the special cropping rotation system of wheat and maize requires a suitable reproductive period for maize. The AUX/IAA and ARF transcription factors are two key genes that regulate auxin signals in plants, which play an important role during the process of auxin regulation in plant growth and development. In this study, we obtained two overexpressed lines of <i>ZmIAA29</i> belonging to the AUX/IAA transcription factor family. Compared with the Wide type, the overexpressed strains of <i>ZmIAA29</i> were significantly regulated in maize during the flowering time and had increased ear weight. Moreover, ZmIAA29 was assayed via the yeast two-hybrid and BiLUC systems for interaction, and the results showed that ZmIAA29 interacted with several ZmARF transcription factors. Furthermore, RNA-seq and DAP-seq analysis revealed that <i>ZmIAA29</i> regulates maize growth and development by influencing pathways such as those for plant signal transduction and the key genes related to maize flowering. Taken together, our results suggest that ZmIAA29 plays a key role in the development of maize flowering. This study provides new insight for better understanding the combined regulation of the AUX/IAA and ARF transcription factors during flowering time in maize. |
| first_indexed | 2024-03-11T00:12:20Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T00:12:20Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-452ea1bc609b4b80917a55b88f084dfc2023-11-18T23:54:02ZengMDPI AGAgronomy2073-43952023-07-01138202810.3390/agronomy13082028Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering TimeChenhui Ma0Kuntai Dang1Qiankun Xie2Javed Hussain Sahito3Baiyu Yuan4Jiong Wan5Xiaoqian Qiu6Jiawen Zhao7Yanan Lin8Shujun Meng9Liqin Mu10Dong Ding11Huili Yang12Yadong Xue13Xiaoyang Chen14Xuehai Zhang15Jihua Tang16National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaMaize is one of the most important food and feed crops in China due to the high demand for its use by both humans and animals. The Huang-Huai-Hai region is a major maize cultivation area in China, and the special cropping rotation system of wheat and maize requires a suitable reproductive period for maize. The AUX/IAA and ARF transcription factors are two key genes that regulate auxin signals in plants, which play an important role during the process of auxin regulation in plant growth and development. In this study, we obtained two overexpressed lines of <i>ZmIAA29</i> belonging to the AUX/IAA transcription factor family. Compared with the Wide type, the overexpressed strains of <i>ZmIAA29</i> were significantly regulated in maize during the flowering time and had increased ear weight. Moreover, ZmIAA29 was assayed via the yeast two-hybrid and BiLUC systems for interaction, and the results showed that ZmIAA29 interacted with several ZmARF transcription factors. Furthermore, RNA-seq and DAP-seq analysis revealed that <i>ZmIAA29</i> regulates maize growth and development by influencing pathways such as those for plant signal transduction and the key genes related to maize flowering. Taken together, our results suggest that ZmIAA29 plays a key role in the development of maize flowering. This study provides new insight for better understanding the combined regulation of the AUX/IAA and ARF transcription factors during flowering time in maize.https://www.mdpi.com/2073-4395/13/8/2028AUX/IAA TFARF TFflorescence |
| spellingShingle | Chenhui Ma Kuntai Dang Qiankun Xie Javed Hussain Sahito Baiyu Yuan Jiong Wan Xiaoqian Qiu Jiawen Zhao Yanan Lin Shujun Meng Liqin Mu Dong Ding Huili Yang Yadong Xue Xiaoyang Chen Xuehai Zhang Jihua Tang Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time Agronomy AUX/IAA TF ARF TF florescence |
| title | Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time |
| title_full | Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time |
| title_fullStr | Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time |
| title_full_unstemmed | Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time |
| title_short | Over-Expression of <i>ZmIAA29</i>, an AUX/IAA Transcription Factor, Improved Maize Flowering Time |
| title_sort | over expression of i zmiaa29 i an aux iaa transcription factor improved maize flowering time |
| topic | AUX/IAA TF ARF TF florescence |
| url | https://www.mdpi.com/2073-4395/13/8/2028 |
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