Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians
Abstract Natural killer (NK) cells are crucial effector cells of the innate immune response to viral infections, including HIV, through cytolytic activity and the production of cytokines with anti-HIV activities. We recruited 15 treatment naïve HIV patients and 16 healthy controls (HC) to assess NK...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18413-3 |
| _version_ | 1811297357445201920 |
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| author | Henok Andualem Mulualem Lemma Amare Keflie Meseret Workeneh Birhanu Ayelign Yayehyirad Tassachew Lidya Hailu Alene Geteneh Adane Mihret Martha Zewdie Rawleigh Howe |
| author_facet | Henok Andualem Mulualem Lemma Amare Keflie Meseret Workeneh Birhanu Ayelign Yayehyirad Tassachew Lidya Hailu Alene Geteneh Adane Mihret Martha Zewdie Rawleigh Howe |
| author_sort | Henok Andualem |
| collection | DOAJ |
| description | Abstract Natural killer (NK) cells are crucial effector cells of the innate immune response to viral infections, including HIV, through cytolytic activity and the production of cytokines with anti-HIV activities. We recruited 15 treatment naïve HIV patients and 16 healthy controls (HC) to assess NK cell subsets or expression of multiple markers by flow cytometry. The frequency of circulating CD56brightCD16−ve and CD56dimCD16bright NK cell subsets was significantly lower among the HIV group than in HC. The CD56−veCD16bright subset was higher in HIV patients, but this was only apparent when gated among total NK cells, not total lymphocytes. NK cells among HIV participants also showed a lower and higher frequency of CD8 and HLA-DR expressing cells, respectively. In addition, CD7 median fluorescent intensity and CD2+CD7− frequencies were significantly lower in HIV patients. A distinct population of KIR3DL1/S1 cells was unexpectedly higher among CD56brightCD16−ve NK cells in HIV patients. In conclusion, this study in the Ethiopian setting confirms many previous findings, but the down-regulation of CD7 and enhanced KIR3DL1/S1 within the CD56bright subsets have not been widely reported among HIV patients and merit further research. |
| first_indexed | 2024-04-13T06:03:04Z |
| format | Article |
| id | doaj.art-45313548a5fd454780530ab23cf283a9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T06:03:04Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-45313548a5fd454780530ab23cf283a92022-12-22T02:59:21ZengNature PortfolioScientific Reports2045-23222022-08-0112111010.1038/s41598-022-18413-3Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected EthiopiansHenok Andualem0Mulualem Lemma1Amare Keflie2Meseret Workeneh3Birhanu Ayelign4Yayehyirad Tassachew5Lidya Hailu6Alene Geteneh7Adane Mihret8Martha Zewdie9Rawleigh Howe10Department of Medical Laboratory Science, College of Medicine and Health Sciences, Debre Tabor UniversityDepartment of Immunology and Molecular Biology, School of Medical Laboratory Science, College of Medicine and Health Sciences, University of GondarDepartment of Immunology and Molecular Biology, School of Medical Laboratory Science, College of Medicine and Health Sciences, University of GondarDepartment of Immunology and Molecular Biology, School of Medical Laboratory Science, College of Medicine and Health Sciences, University of GondarDepartment of Immunology and Molecular Biology, School of Medical Laboratory Science, College of Medicine and Health Sciences, University of GondarSchool of Medical Laboratory Sciences, College of Medicine and Health Sciences, Hawassa UniversityDepartment of Medical Laboratory Science, College of Medicine and Health Sciences, Debre Tabor UniversityDepartment of Medical Laboratory Science, College of Health Sciences, Woldia UniversityArmauer Hansen Research InstituteArmauer Hansen Research InstituteArmauer Hansen Research InstituteAbstract Natural killer (NK) cells are crucial effector cells of the innate immune response to viral infections, including HIV, through cytolytic activity and the production of cytokines with anti-HIV activities. We recruited 15 treatment naïve HIV patients and 16 healthy controls (HC) to assess NK cell subsets or expression of multiple markers by flow cytometry. The frequency of circulating CD56brightCD16−ve and CD56dimCD16bright NK cell subsets was significantly lower among the HIV group than in HC. The CD56−veCD16bright subset was higher in HIV patients, but this was only apparent when gated among total NK cells, not total lymphocytes. NK cells among HIV participants also showed a lower and higher frequency of CD8 and HLA-DR expressing cells, respectively. In addition, CD7 median fluorescent intensity and CD2+CD7− frequencies were significantly lower in HIV patients. A distinct population of KIR3DL1/S1 cells was unexpectedly higher among CD56brightCD16−ve NK cells in HIV patients. In conclusion, this study in the Ethiopian setting confirms many previous findings, but the down-regulation of CD7 and enhanced KIR3DL1/S1 within the CD56bright subsets have not been widely reported among HIV patients and merit further research.https://doi.org/10.1038/s41598-022-18413-3 |
| spellingShingle | Henok Andualem Mulualem Lemma Amare Keflie Meseret Workeneh Birhanu Ayelign Yayehyirad Tassachew Lidya Hailu Alene Geteneh Adane Mihret Martha Zewdie Rawleigh Howe Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians Scientific Reports |
| title | Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians |
| title_full | Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians |
| title_fullStr | Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians |
| title_full_unstemmed | Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians |
| title_short | Elevated KIR expression and diminished intensity of CD7 on NK cell subsets among treatment naïve HIV infected Ethiopians |
| title_sort | elevated kir expression and diminished intensity of cd7 on nk cell subsets among treatment naive hiv infected ethiopians |
| url | https://doi.org/10.1038/s41598-022-18413-3 |
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