Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code
MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/12/1033 |
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| author | Giulia Tomagra Alice Re Veronica Varzi Pietro Aprà Adam Britel Claudio Franchino Sofia Sturari Nour-Hanne Amine Remco H. S. Westerink Valentina Carabelli Federico Picollo |
| author_facet | Giulia Tomagra Alice Re Veronica Varzi Pietro Aprà Adam Britel Claudio Franchino Sofia Sturari Nour-Hanne Amine Remco H. S. Westerink Valentina Carabelli Federico Picollo |
| author_sort | Giulia Tomagra |
| collection | DOAJ |
| description | MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them recording large amounts of data revealing the exocytotic activity, the aim of this work was to support an adequate analysis code to speed up the signal detection. The cutting-edge technology of microGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) has been implemented with an automated analysis code (APE, Amperometric Peak Analysis) developed using Matlab R2022a software to provide easy and accurate detection of amperometric spike parameters, including the analysis of the pre-spike foot that sometimes precedes the complete fusion pore dilatation. Data have been acquired from cultured PC12 cells, either collecting events during spontaneous exocytosis or after L-DOPA incubation. Validation of the APE code was performed by comparing the acquired spike parameters with those obtained using Quanta Analysis (Igor macro) by Mosharov et al. |
| first_indexed | 2024-03-08T20:56:37Z |
| format | Article |
| id | doaj.art-4531c5589f8d46369a6956802afc7efc |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-08T20:56:37Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-4531c5589f8d46369a6956802afc7efc2023-12-22T13:56:26ZengMDPI AGBiosensors2079-63742023-12-011312103310.3390/bios13121033Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis CodeGiulia Tomagra0Alice Re1Veronica Varzi2Pietro Aprà3Adam Britel4Claudio Franchino5Sofia Sturari6Nour-Hanne Amine7Remco H. S. Westerink8Valentina Carabelli9Federico Picollo10Department of Drug and Science Technology, NIS Interdepartmental Centre, University of Torino, Corso Raffaello 30, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyDepartment of Drug and Science Technology, NIS Interdepartmental Centre, University of Torino, Corso Raffaello 30, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyNeurotoxicology Research Group, Division of Toxicology, Institute for Risk Assessment Sciences (IRAS), Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.177, NL-3508 TD Utrecht, The NetherlandsDepartment of Drug and Science Technology, NIS Interdepartmental Centre, University of Torino, Corso Raffaello 30, 10125 Torino, ItalyDepartment of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, ItalyMicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them recording large amounts of data revealing the exocytotic activity, the aim of this work was to support an adequate analysis code to speed up the signal detection. The cutting-edge technology of microGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) has been implemented with an automated analysis code (APE, Amperometric Peak Analysis) developed using Matlab R2022a software to provide easy and accurate detection of amperometric spike parameters, including the analysis of the pre-spike foot that sometimes precedes the complete fusion pore dilatation. Data have been acquired from cultured PC12 cells, either collecting events during spontaneous exocytosis or after L-DOPA incubation. Validation of the APE code was performed by comparing the acquired spike parameters with those obtained using Quanta Analysis (Igor macro) by Mosharov et al.https://www.mdpi.com/2079-6374/13/12/1033exocytosisdiamondmulti-electrode arrayPC12 cell lineamperometrydopamine |
| spellingShingle | Giulia Tomagra Alice Re Veronica Varzi Pietro Aprà Adam Britel Claudio Franchino Sofia Sturari Nour-Hanne Amine Remco H. S. Westerink Valentina Carabelli Federico Picollo Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code Biosensors exocytosis diamond multi-electrode array PC12 cell line amperometry dopamine |
| title | Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code |
| title_full | Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code |
| title_fullStr | Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code |
| title_full_unstemmed | Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code |
| title_short | Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code |
| title_sort | enhancing the study of quantal exocytotic events combining diamond multi electrode arrays with amperometric peak analysis ape an automated analysis code |
| topic | exocytosis diamond multi-electrode array PC12 cell line amperometry dopamine |
| url | https://www.mdpi.com/2079-6374/13/12/1033 |
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