Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.
Main Authors: | Hagar Meltzer, Efrat Marom, Idan Alyagor, Oded Mayseless, Victoria Berkun, Netta Segal-Gilboa, Tamar Unger, David Luginbuhl, Oren Schuldiner |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2019-05-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-10140-0 |
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