Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum
Abstract Background Dourine, a venereal transmitted trypanosomosis caused by Trypanosoma equiperdum, has different clinical signs related to the reproductive and nervous system. Pathologic tissue changes associated with the disease are poorly described. The present study describes the histopathologi...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-019-1916-7 |
| _version_ | 1818171627176722432 |
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| author | Ahmed Yasine Hagos Ashenafi Peter Geldhof Leen Van Brantegem Griet Vercauteren Merga Bekana Alemu Tola Ann Van Soom Luc Duchateau Bruno Goddeeris Jan Govaere |
| author_facet | Ahmed Yasine Hagos Ashenafi Peter Geldhof Leen Van Brantegem Griet Vercauteren Merga Bekana Alemu Tola Ann Van Soom Luc Duchateau Bruno Goddeeris Jan Govaere |
| author_sort | Ahmed Yasine |
| collection | DOAJ |
| description | Abstract Background Dourine, a venereal transmitted trypanosomosis caused by Trypanosoma equiperdum, has different clinical signs related to the reproductive and nervous system. Pathologic tissue changes associated with the disease are poorly described. The present study describes the histopathological lesions in naturally T. equiperdum-infected horses in the chronical stage of dourine. Results Four chronically dourine diseased horses underwent a post-mortem examination. They were Woo test negative, but CATT/T. evansi positive, had a low packed cell volume (PCV) and exhibited obvious clinical signs of dourine. Post-mortem examination did not reveal gross lesions in the organs assumed to be responsible for the symptomatology. On histopathology, genital organs were affected, with mononuclear cell infiltration and erosions and degeneration of seminiferous tubules and perivascular lymphoplasmacytic cuffing in the uterus. In the nervous system, mononuclear cell infiltration was located in peripheral nerves, ganglia and in the spinal cord, leading to axonal degeneration. Real-time PCR using ITS primer revealed the presence of trypanosomes in these organs and conventional PCRs using maxicircle and RoTat1.2 primers further confirmed the involvement of T. equiperdum since the DNAs from the vagina, testicle, distal spinal cord, sciatic and obturator nerves found to be positive for maxicircle and negative for RoTat 1.2. Conclusions The histopathological lesions in the spinal cord and peripheral nerves explain the incoordination of the hind legs in T. equiperdum-infected horses, whilst its presence in the genital tract exemplifies the venereal transmission. |
| first_indexed | 2024-12-11T18:59:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-11T18:59:43Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-4536d2317b9847a29b64d0864af568742022-12-22T00:54:03ZengBMCBMC Veterinary Research1746-61482019-05-0115111010.1186/s12917-019-1916-7Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdumAhmed Yasine0Hagos Ashenafi1Peter Geldhof2Leen Van Brantegem3Griet Vercauteren4Merga Bekana5Alemu Tola6Ann Van Soom7Luc Duchateau8Bruno Goddeeris9Jan Govaere10Wollo University, School of Veterinary MedicineAddis Ababa University, College of Veterinary MedicineGhent University, Faculty of Veterinary MedicineGhent University, Faculty of Veterinary MedicineVet-Path bvbaAddis Ababa University, College of Veterinary MedicineAddis Ababa University, College of Veterinary MedicineGhent University, Faculty of Veterinary MedicineGhent University, Faculty of Veterinary MedicineGhent University, Faculty of Veterinary MedicineGhent University, Faculty of Veterinary MedicineAbstract Background Dourine, a venereal transmitted trypanosomosis caused by Trypanosoma equiperdum, has different clinical signs related to the reproductive and nervous system. Pathologic tissue changes associated with the disease are poorly described. The present study describes the histopathological lesions in naturally T. equiperdum-infected horses in the chronical stage of dourine. Results Four chronically dourine diseased horses underwent a post-mortem examination. They were Woo test negative, but CATT/T. evansi positive, had a low packed cell volume (PCV) and exhibited obvious clinical signs of dourine. Post-mortem examination did not reveal gross lesions in the organs assumed to be responsible for the symptomatology. On histopathology, genital organs were affected, with mononuclear cell infiltration and erosions and degeneration of seminiferous tubules and perivascular lymphoplasmacytic cuffing in the uterus. In the nervous system, mononuclear cell infiltration was located in peripheral nerves, ganglia and in the spinal cord, leading to axonal degeneration. Real-time PCR using ITS primer revealed the presence of trypanosomes in these organs and conventional PCRs using maxicircle and RoTat1.2 primers further confirmed the involvement of T. equiperdum since the DNAs from the vagina, testicle, distal spinal cord, sciatic and obturator nerves found to be positive for maxicircle and negative for RoTat 1.2. Conclusions The histopathological lesions in the spinal cord and peripheral nerves explain the incoordination of the hind legs in T. equiperdum-infected horses, whilst its presence in the genital tract exemplifies the venereal transmission.http://link.springer.com/article/10.1186/s12917-019-1916-7DourineT. EquiperdumHorsesPCRHistopathology |
| spellingShingle | Ahmed Yasine Hagos Ashenafi Peter Geldhof Leen Van Brantegem Griet Vercauteren Merga Bekana Alemu Tola Ann Van Soom Luc Duchateau Bruno Goddeeris Jan Govaere Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum BMC Veterinary Research Dourine T. Equiperdum Horses PCR Histopathology |
| title | Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum |
| title_full | Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum |
| title_fullStr | Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum |
| title_full_unstemmed | Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum |
| title_short | Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum |
| title_sort | histopathological lesions in reproductive organs distal spinal cord and peripheral nerves of horses naturally infected with trypanosoma equiperdum |
| topic | Dourine T. Equiperdum Horses PCR Histopathology |
| url | http://link.springer.com/article/10.1186/s12917-019-1916-7 |
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