Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages.
RNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-02-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0011055 |
| _version_ | 1797860077133103104 |
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| author | Glenn Oliveira Chantal B F Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P Leon Carlos O Ontiveros Rimjhim Agarwal Konstantin A Tsetsarkin Eva Harris Gregory D Ebel Shirlee Wohl Nathan D Grubaugh Kristian G Andersen |
| author_facet | Glenn Oliveira Chantal B F Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P Leon Carlos O Ontiveros Rimjhim Agarwal Konstantin A Tsetsarkin Eva Harris Gregory D Ebel Shirlee Wohl Nathan D Grubaugh Kristian G Andersen |
| author_sort | Glenn Oliveira |
| collection | DOAJ |
| description | RNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the 2015-2016 Zika epidemic in the Americas for lineage-specific fitness differences. We engineered a library of recombinant viruses representing twelve major Zika virus lineages and used them to measure replicative fitness within disease-relevant human primary cells and live mosquitoes. We found that two of these lineages conferred significant in vitro replicative fitness changes among human primary cells, but we did not find fitness changes in Aedes aegypti mosquitoes. Additionally, we found evidence for elevated levels of positive selection among five amino acid sites that define major Zika virus lineages. While our work suggests that Zika virus may have acquired several phenotypic changes during a short time scale, these changes were relatively moderate and do not appear to have enhanced transmission during the epidemic. |
| first_indexed | 2024-04-09T21:41:05Z |
| format | Article |
| id | doaj.art-453bfc6452a142fa8fc5e58577519836 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-09T21:41:05Z |
| publishDate | 2023-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-453bfc6452a142fa8fc5e585775198362023-03-26T05:32:19ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352023-02-01172e001105510.1371/journal.pntd.0011055Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages.Glenn OliveiraChantal B F VogelsAshley ZolfaghariSharada SarafRaphaelle KlittingJames Weger-LucarelliKarla P LeonCarlos O OntiverosRimjhim AgarwalKonstantin A TsetsarkinEva HarrisGregory D EbelShirlee WohlNathan D GrubaughKristian G AndersenRNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the 2015-2016 Zika epidemic in the Americas for lineage-specific fitness differences. We engineered a library of recombinant viruses representing twelve major Zika virus lineages and used them to measure replicative fitness within disease-relevant human primary cells and live mosquitoes. We found that two of these lineages conferred significant in vitro replicative fitness changes among human primary cells, but we did not find fitness changes in Aedes aegypti mosquitoes. Additionally, we found evidence for elevated levels of positive selection among five amino acid sites that define major Zika virus lineages. While our work suggests that Zika virus may have acquired several phenotypic changes during a short time scale, these changes were relatively moderate and do not appear to have enhanced transmission during the epidemic.https://doi.org/10.1371/journal.pntd.0011055 |
| spellingShingle | Glenn Oliveira Chantal B F Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P Leon Carlos O Ontiveros Rimjhim Agarwal Konstantin A Tsetsarkin Eva Harris Gregory D Ebel Shirlee Wohl Nathan D Grubaugh Kristian G Andersen Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. PLoS Neglected Tropical Diseases |
| title | Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. |
| title_full | Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. |
| title_fullStr | Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. |
| title_full_unstemmed | Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. |
| title_short | Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. |
| title_sort | genomic and phenotypic analyses suggest moderate fitness differences among zika virus lineages |
| url | https://doi.org/10.1371/journal.pntd.0011055 |
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