Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties
To cope with the yield loss caused by drought stress, new oat varieties with greater drought tolerance need to be selected. In this study, two oat varieties with different drought tolerances were selected for analysis of their phenotypes and physiological indices under moderate and severe soil droug...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/2/177 |
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| author | Weiwei Xu Laichun Guo Chunlong Wang Liming Wei Qiang Wang Qinyong Ren Xiwu Yang Chao Zhan Xiaotian Liang Junying Wang Changzhong Ren |
| author_facet | Weiwei Xu Laichun Guo Chunlong Wang Liming Wei Qiang Wang Qinyong Ren Xiwu Yang Chao Zhan Xiaotian Liang Junying Wang Changzhong Ren |
| author_sort | Weiwei Xu |
| collection | DOAJ |
| description | To cope with the yield loss caused by drought stress, new oat varieties with greater drought tolerance need to be selected. In this study, two oat varieties with different drought tolerances were selected for analysis of their phenotypes and physiological indices under moderate and severe soil drought stress. The results revealed significant differences in the degree of wilting, leaf relative water content (RWC), and SOD and CAT activity between the two oat genotypes under severe soil drought stress; moreover, the drought-tolerant variety exhibited a significant increase in the number of stomata and wax crystals on the surface of both the leaf and guard cells; additionally, the morphology of the guard cells was normal, and there was no significant disruption of the grana lamella membrane or the nuclear envelope. Furthermore, transcriptome analysis revealed that the expression of genes related to the biosynthesis of waxes and cell-wall components, as well as those of the WRKY family, significantly increased in the drought-tolerant variety. These findings suggest that several genes involved in the antioxidant pathway could improve drought tolerance in plants by regulating the increase/decrease in wax and cell-wall constituents and maintaining normal cellular water potential, as well as improving the ability of the antioxidant system to scavenge peroxides in oats. |
| first_indexed | 2024-03-08T09:48:36Z |
| format | Article |
| id | doaj.art-453e06925c124f329abb33494da8b22d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T09:48:36Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-453e06925c124f329abb33494da8b22d2024-01-29T14:10:43ZengMDPI AGPlants2223-77472024-01-0113217710.3390/plants13020177Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) VarietiesWeiwei Xu0Laichun Guo1Chunlong Wang2Liming Wei3Qiang Wang4Qinyong Ren5Xiwu Yang6Chao Zhan7Xiaotian Liang8Junying Wang9Changzhong Ren10Agronomy College, Jilin Agricultural University, Changchun 130118, ChinaNational Oat Improvement Center, Baicheng Academy of Agricultural Sciences, Baicheng 137000, ChinaNational Oat Improvement Center, Baicheng Academy of Agricultural Sciences, Baicheng 137000, ChinaNational Oat Improvement Center, Baicheng Academy of Agricultural Sciences, Baicheng 137000, ChinaNational Oat Improvement Center, Baicheng Academy of Agricultural Sciences, Baicheng 137000, ChinaAgronomy College, Jilin Agricultural University, Changchun 130118, ChinaAgronomy College, Jilin Agricultural University, Changchun 130118, ChinaNational Oat Improvement Center, Baicheng Academy of Agricultural Sciences, Baicheng 137000, ChinaAgronomy College, Jilin Agricultural University, Changchun 130118, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaAgronomy College, Jilin Agricultural University, Changchun 130118, ChinaTo cope with the yield loss caused by drought stress, new oat varieties with greater drought tolerance need to be selected. In this study, two oat varieties with different drought tolerances were selected for analysis of their phenotypes and physiological indices under moderate and severe soil drought stress. The results revealed significant differences in the degree of wilting, leaf relative water content (RWC), and SOD and CAT activity between the two oat genotypes under severe soil drought stress; moreover, the drought-tolerant variety exhibited a significant increase in the number of stomata and wax crystals on the surface of both the leaf and guard cells; additionally, the morphology of the guard cells was normal, and there was no significant disruption of the grana lamella membrane or the nuclear envelope. Furthermore, transcriptome analysis revealed that the expression of genes related to the biosynthesis of waxes and cell-wall components, as well as those of the WRKY family, significantly increased in the drought-tolerant variety. These findings suggest that several genes involved in the antioxidant pathway could improve drought tolerance in plants by regulating the increase/decrease in wax and cell-wall constituents and maintaining normal cellular water potential, as well as improving the ability of the antioxidant system to scavenge peroxides in oats.https://www.mdpi.com/2223-7747/13/2/177oatsoil drought stressleaf ultrastructurecell wall |
| spellingShingle | Weiwei Xu Laichun Guo Chunlong Wang Liming Wei Qiang Wang Qinyong Ren Xiwu Yang Chao Zhan Xiaotian Liang Junying Wang Changzhong Ren Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties Plants oat soil drought stress leaf ultrastructure cell wall |
| title | Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties |
| title_full | Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties |
| title_fullStr | Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties |
| title_full_unstemmed | Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties |
| title_short | Transcriptome Analysis Reveals Drought-Responsive Pathways and Key Genes of Two Oat (<i>Avena sativa</i>) Varieties |
| title_sort | transcriptome analysis reveals drought responsive pathways and key genes of two oat i avena sativa i varieties |
| topic | oat soil drought stress leaf ultrastructure cell wall |
| url | https://www.mdpi.com/2223-7747/13/2/177 |
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