Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats
The aim of this study is to investigate the effects of ovariectomy on bone mineral density (BMD) and oxidative state in rats, and the alterations in these effects that vitamin C supplementation may produce. Twenty female Wistar albino rats were randomly divided into three groups: control (C, n=6); o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2011-10-01
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| Series: | Libyan Journal of Medicine |
| Subjects: | |
| Online Access: | http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/5965/pdf |
| _version_ | 1818854640272277504 |
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| author | Mustafa Arslan Aliye Tosun Isik Keles Gülümser Aydin Sevim Orkun Ayse Arslan Osman Caglayan |
| author_facet | Mustafa Arslan Aliye Tosun Isik Keles Gülümser Aydin Sevim Orkun Ayse Arslan Osman Caglayan |
| author_sort | Mustafa Arslan |
| collection | DOAJ |
| description | The aim of this study is to investigate the effects of ovariectomy on bone mineral density (BMD) and oxidative state in rats, and the alterations in these effects that vitamin C supplementation may produce. Twenty female Wistar albino rats were randomly divided into three groups: control (C, n=6); ovariectomy (O, n=7); and ovariectomy + vitamin C supplement (OV, n=7). Oxidative stress (OS) was assessed 100 days postovariectomy by measuring the activity of several enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase, as well as the concentrations of malondialdehyde (MDA), nitric oxide (NO), and total sulfhydryl groups in plasma and bone homogenates. A significant decrease in BMD was observed in O group compared with C group (p = 0.015), and a significant increase was observed in OV compared with O group (p=0.003). When groups were compared with respect to parameters of OS, MDA and NO levels in bone tissue were significantly higher in O than in C (p=0.032, p=0.022) and were significantly lower in OV than in O (p=0.025, p=0.018). SOD activity was significantly higher in O than in C (p=0.032). In plasma, MDA activity was significantly higher in O than in C (p = 0.022) and NO level was significantly higher in O than in C and OV (p=0.017, p=0.018). Our results suggest that ovariectomy may produce osteoporosis and OS in females, and vitamin C supplementation may provide alterations regarding improvement in OS and BMD values. We assume that studies including more subjects are needed to make a decisive conclusion about OS–BMD relation. |
| first_indexed | 2024-12-19T07:55:55Z |
| format | Article |
| id | doaj.art-453ffb973013475881aa9cfb54337d7a |
| institution | Directory Open Access Journal |
| issn | 1819-6357 |
| language | English |
| last_indexed | 2024-12-19T07:55:55Z |
| publishDate | 2011-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Libyan Journal of Medicine |
| spelling | doaj.art-453ffb973013475881aa9cfb54337d7a2022-12-21T20:30:00ZengTaylor & Francis GroupLibyan Journal of Medicine1819-63572011-10-01601910.3402/ljm.v6i0.5965Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in ratsMustafa ArslanAliye TosunIsik KelesGülümser AydinSevim OrkunAyse ArslanOsman CaglayanThe aim of this study is to investigate the effects of ovariectomy on bone mineral density (BMD) and oxidative state in rats, and the alterations in these effects that vitamin C supplementation may produce. Twenty female Wistar albino rats were randomly divided into three groups: control (C, n=6); ovariectomy (O, n=7); and ovariectomy + vitamin C supplement (OV, n=7). Oxidative stress (OS) was assessed 100 days postovariectomy by measuring the activity of several enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase, as well as the concentrations of malondialdehyde (MDA), nitric oxide (NO), and total sulfhydryl groups in plasma and bone homogenates. A significant decrease in BMD was observed in O group compared with C group (p = 0.015), and a significant increase was observed in OV compared with O group (p=0.003). When groups were compared with respect to parameters of OS, MDA and NO levels in bone tissue were significantly higher in O than in C (p=0.032, p=0.022) and were significantly lower in OV than in O (p=0.025, p=0.018). SOD activity was significantly higher in O than in C (p=0.032). In plasma, MDA activity was significantly higher in O than in C (p = 0.022) and NO level was significantly higher in O than in C and OV (p=0.017, p=0.018). Our results suggest that ovariectomy may produce osteoporosis and OS in females, and vitamin C supplementation may provide alterations regarding improvement in OS and BMD values. We assume that studies including more subjects are needed to make a decisive conclusion about OS–BMD relation.http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/5965/pdfratovariectomyosteoporosisoxidative stressvitamin Cascorbic |
| spellingShingle | Mustafa Arslan Aliye Tosun Isik Keles Gülümser Aydin Sevim Orkun Ayse Arslan Osman Caglayan Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats Libyan Journal of Medicine rat ovariectomy osteoporosis oxidative stress vitamin C ascorbic |
| title | Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats |
| title_full | Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats |
| title_fullStr | Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats |
| title_full_unstemmed | Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats |
| title_short | Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats |
| title_sort | effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats |
| topic | rat ovariectomy osteoporosis oxidative stress vitamin C ascorbic |
| url | http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/5965/pdf |
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