Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms
Consumption of contaminated drinking water heavily contributes to the burden of gastrointestinal waterborne diseases. Conventional detection methodologies present several shortcomings, such as indirect measure of indicator species, low throughput and time consume. DNA chips have the potential to ser...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-03-01
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| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/mr/article/view/3855 |
| _version_ | 1818333186006974464 |
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| author | Filipa F. Vale Kathy D. Saraiva-Pava Magda S.C. Fontes Helena Vieira |
| author_facet | Filipa F. Vale Kathy D. Saraiva-Pava Magda S.C. Fontes Helena Vieira |
| author_sort | Filipa F. Vale |
| collection | DOAJ |
| description | Consumption of contaminated drinking water heavily contributes to the burden of gastrointestinal waterborne diseases. Conventional detection methodologies present several shortcomings, such as indirect measure of indicator species, low throughput and time consume. DNA chips have the potential to serve as surveillance systems for the simultaneous detection of pathogens overcoming these limitations. We have developed a DNA chip for simultaneous detection of multiple waterborne pathogens. Species specific DNA probes were implemented on a microarray, for microorganism detection. Present study reports the results of one untreated water sample analyzed by conventional methods and by the DNA chip. The results were concordant for the mandatory organisms (total coliforms, <em>Escherichia coli</em>, fecal enterococci) using both methods, reinforcing the utility and proof-ofconcept of the DNA chip. However, it is necessary a prior enrichment in a culture medium in order to obtain a positive signal using the DNA chip. The DNA chip may be a valuable distinctive tool for waterborne pathogens detection |
| first_indexed | 2024-12-13T13:47:38Z |
| format | Article |
| id | doaj.art-45424db3b80247ae9f874a2ec905604b |
| institution | Directory Open Access Journal |
| issn | 2036-7473 2036-7481 |
| language | English |
| last_indexed | 2024-12-13T13:47:38Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-45424db3b80247ae9f874a2ec905604b2022-12-21T23:43:21ZengMDPI AGMicrobiology Research2036-74732036-74812012-03-0131e11e1110.4081/mr.2012.e112078Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganismsFilipa F. Vale0Kathy D. Saraiva-Pava1Magda S.C. Fontes2Helena Vieira3Faculty of Engineering, Catholic University of Portugal, Rio de MouroFaculty of Engineering, Catholic University of Portugal, Rio de MouroFaculty of Engineering, Catholic University of Portugal, Rio de MouroBioalvo S.A., Edificio ICAT; Universidade de Lisboa, Facultade de Ciencias, Centre for Biodiversity, Functional and Integrative Genomics (BioFIG), Campus de FCUL, Campo Grande, LisbonConsumption of contaminated drinking water heavily contributes to the burden of gastrointestinal waterborne diseases. Conventional detection methodologies present several shortcomings, such as indirect measure of indicator species, low throughput and time consume. DNA chips have the potential to serve as surveillance systems for the simultaneous detection of pathogens overcoming these limitations. We have developed a DNA chip for simultaneous detection of multiple waterborne pathogens. Species specific DNA probes were implemented on a microarray, for microorganism detection. Present study reports the results of one untreated water sample analyzed by conventional methods and by the DNA chip. The results were concordant for the mandatory organisms (total coliforms, <em>Escherichia coli</em>, fecal enterococci) using both methods, reinforcing the utility and proof-ofconcept of the DNA chip. However, it is necessary a prior enrichment in a culture medium in order to obtain a positive signal using the DNA chip. The DNA chip may be a valuable distinctive tool for waterborne pathogens detectionhttp://www.pagepress.org/journals/index.php/mr/article/view/3855DNA chipsimultaneous microorganism’s detectionwater qualityindicator bacteriawaterborne pathogen |
| spellingShingle | Filipa F. Vale Kathy D. Saraiva-Pava Magda S.C. Fontes Helena Vieira Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms Microbiology Research DNA chip simultaneous microorganism’s detection water quality indicator bacteria waterborne pathogen |
| title | Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms |
| title_full | Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms |
| title_fullStr | Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms |
| title_full_unstemmed | Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms |
| title_short | Microbiologic quality of an untreated water sample analyzed by a novel DNA chip for simultaneous detection of microorganisms |
| title_sort | microbiologic quality of an untreated water sample analyzed by a novel dna chip for simultaneous detection of microorganisms |
| topic | DNA chip simultaneous microorganism’s detection water quality indicator bacteria waterborne pathogen |
| url | http://www.pagepress.org/journals/index.php/mr/article/view/3855 |
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