Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis.
WNT signaling mediates various physiological and pathological processes. We previously showed that WNT10A is a novel angio/stromagenic factor involved in such processes as tumor growth, wound healing and tissue fibrosis. In this study, we investigated the role of WNT10A in promoting the fibrosis tha...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4108433?pdf=render |
| _version_ | 1819131411663159296 |
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| author | Akihiro Kuma Sohsuke Yamada Ke-Yong Wang Noriaki Kitamura Takahiro Yamaguchi Yoshiko Iwai Hiroto Izumi Masahito Tamura Yutaka Otsuji Kimitoshi Kohno |
| author_facet | Akihiro Kuma Sohsuke Yamada Ke-Yong Wang Noriaki Kitamura Takahiro Yamaguchi Yoshiko Iwai Hiroto Izumi Masahito Tamura Yutaka Otsuji Kimitoshi Kohno |
| author_sort | Akihiro Kuma |
| collection | DOAJ |
| description | WNT signaling mediates various physiological and pathological processes. We previously showed that WNT10A is a novel angio/stromagenic factor involved in such processes as tumor growth, wound healing and tissue fibrosis. In this study, we investigated the role of WNT10A in promoting the fibrosis that is central to the pathology of acute interstitial nephritis (AIN). We initially asked whether there is an association between kidney function (estimated glomerular filtration rate; eGFR) and WNT10A expression using kidney biopsies from 20 patients with AIN. Interestingly, patients with WNT10A expression had significantly lower eGFR than WNT10A-negative patients. However, changes in kidney function were not related to the level of expression of other WNT family members. Furthermore, there was positive correlation between WNT10A and α-SMA expression. We next investigated the involvement of WNT10A in kidney fibrosis processes using COS1 cells, a kidney fibroblast cell line. WNT10A overexpression increased the level of expression of fibronectin and peroxiredoxin 5. Furthermore, WNT10A overexpression renders cells resistant to apoptosis induced by hydrogen peroxide and high glucose. Collectively, WNT10A may induce kidney fibrosis and associate with kidney dysfunction in AIN. |
| first_indexed | 2024-12-22T09:15:05Z |
| format | Article |
| id | doaj.art-45437acca84a46e1b441d9098e090950 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T09:15:05Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-45437acca84a46e1b441d9098e0909502022-12-21T18:31:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10324010.1371/journal.pone.0103240Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis.Akihiro KumaSohsuke YamadaKe-Yong WangNoriaki KitamuraTakahiro YamaguchiYoshiko IwaiHiroto IzumiMasahito TamuraYutaka OtsujiKimitoshi KohnoWNT signaling mediates various physiological and pathological processes. We previously showed that WNT10A is a novel angio/stromagenic factor involved in such processes as tumor growth, wound healing and tissue fibrosis. In this study, we investigated the role of WNT10A in promoting the fibrosis that is central to the pathology of acute interstitial nephritis (AIN). We initially asked whether there is an association between kidney function (estimated glomerular filtration rate; eGFR) and WNT10A expression using kidney biopsies from 20 patients with AIN. Interestingly, patients with WNT10A expression had significantly lower eGFR than WNT10A-negative patients. However, changes in kidney function were not related to the level of expression of other WNT family members. Furthermore, there was positive correlation between WNT10A and α-SMA expression. We next investigated the involvement of WNT10A in kidney fibrosis processes using COS1 cells, a kidney fibroblast cell line. WNT10A overexpression increased the level of expression of fibronectin and peroxiredoxin 5. Furthermore, WNT10A overexpression renders cells resistant to apoptosis induced by hydrogen peroxide and high glucose. Collectively, WNT10A may induce kidney fibrosis and associate with kidney dysfunction in AIN.http://europepmc.org/articles/PMC4108433?pdf=render |
| spellingShingle | Akihiro Kuma Sohsuke Yamada Ke-Yong Wang Noriaki Kitamura Takahiro Yamaguchi Yoshiko Iwai Hiroto Izumi Masahito Tamura Yutaka Otsuji Kimitoshi Kohno Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis. PLoS ONE |
| title | Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis. |
| title_full | Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis. |
| title_fullStr | Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis. |
| title_full_unstemmed | Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis. |
| title_short | Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis. |
| title_sort | role of wnt10a expressing kidney fibroblasts in acute interstitial nephritis |
| url | http://europepmc.org/articles/PMC4108433?pdf=render |
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