Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke
BackgroundRegulatory T cells (Treg) have been identified as a key modulator of neuroinflammation in stroke. However, little is known about the association of Treg subpopulations with clinical outcome in patients with acute ischemic stroke (AIS).MethodsPatients within 1 week from stroke onset were pr...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1072980/full |
| _version_ | 1797897144241225728 |
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| author | Gang Deng Yue Tang Jun Xiao Xin Chen Yun-Hui Chu Ke Shang Luo-Qi Zhou Chuan Qin Feng Wang Dai-Shi Tian |
| author_facet | Gang Deng Yue Tang Jun Xiao Xin Chen Yun-Hui Chu Ke Shang Luo-Qi Zhou Chuan Qin Feng Wang Dai-Shi Tian |
| author_sort | Gang Deng |
| collection | DOAJ |
| description | BackgroundRegulatory T cells (Treg) have been identified as a key modulator of neuroinflammation in stroke. However, little is known about the association of Treg subpopulations with clinical outcome in patients with acute ischemic stroke (AIS).MethodsPatients within 1 week from stroke onset were prospectively enrolled in this study. Healthy controls were sex-and age-matched 1:1 to AIS patients. The frequencies of Treg and Treg subsets were analyzed by flow cytometry and compared with nonstroke control. Univariate and multivariate logistic regression analysis was performed to investigate the prognostic value of Treg subsets in stroke outcomes.ResultsA total of 328 patients and 328 controls were included in the study. Compared with controls, patients with AIS had higher levels of Treg frequency and memory Treg (mTreg) frequency, but lower levels of naïve Treg (nTreg) frequency and nTreg/mTreg ratio. One hundred twenty-six (38.4%) patients experienced unfavorable outcome (modified Rankin score 2–6). Multivariate regression analysis showed that nTreg/mTreg ratio was negatively associated with unfavorable 90-day outcome (the highest tertile versus the lowest tertile: odds ratio 0.13, 95% confidential interval [CI] 0.05–0.35). The risk estimation of unfavorable 90 day outcome can be significantly improved by adding nTreg/mTreg ratio to the conventional clinical parameters (continuous net reclassification improvement 91.26, 95% CI 69.04–113.5%, p < 0.001; integrated discrimination improvement 22.38, 95% CI 17.16–27.59%, p < 0.001).ConclusionThis study showed that patients with AIS had elevated Treg frequency and mTreg frequency, but reduced nTreg frequency and nTreg/mTreg ratio. Admission nTreg/mTreg ratio was an independent predictor of unfavorable 90 day outcome in AIS. However, large sample-size cohort studies are needed to confirm our findings. |
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| id | doaj.art-45465b7323c5494d81e90f463675ae18 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-04-10T07:54:05Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-45465b7323c5494d81e90f463675ae182023-02-23T07:03:40ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652023-02-011510.3389/fnagi.2023.10729801072980Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic strokeGang Deng0Yue Tang1Jun Xiao2Xin Chen3Yun-Hui Chu4Ke Shang5Luo-Qi Zhou6Chuan Qin7Feng Wang8Dai-Shi Tian9Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaBackgroundRegulatory T cells (Treg) have been identified as a key modulator of neuroinflammation in stroke. However, little is known about the association of Treg subpopulations with clinical outcome in patients with acute ischemic stroke (AIS).MethodsPatients within 1 week from stroke onset were prospectively enrolled in this study. Healthy controls were sex-and age-matched 1:1 to AIS patients. The frequencies of Treg and Treg subsets were analyzed by flow cytometry and compared with nonstroke control. Univariate and multivariate logistic regression analysis was performed to investigate the prognostic value of Treg subsets in stroke outcomes.ResultsA total of 328 patients and 328 controls were included in the study. Compared with controls, patients with AIS had higher levels of Treg frequency and memory Treg (mTreg) frequency, but lower levels of naïve Treg (nTreg) frequency and nTreg/mTreg ratio. One hundred twenty-six (38.4%) patients experienced unfavorable outcome (modified Rankin score 2–6). Multivariate regression analysis showed that nTreg/mTreg ratio was negatively associated with unfavorable 90-day outcome (the highest tertile versus the lowest tertile: odds ratio 0.13, 95% confidential interval [CI] 0.05–0.35). The risk estimation of unfavorable 90 day outcome can be significantly improved by adding nTreg/mTreg ratio to the conventional clinical parameters (continuous net reclassification improvement 91.26, 95% CI 69.04–113.5%, p < 0.001; integrated discrimination improvement 22.38, 95% CI 17.16–27.59%, p < 0.001).ConclusionThis study showed that patients with AIS had elevated Treg frequency and mTreg frequency, but reduced nTreg frequency and nTreg/mTreg ratio. Admission nTreg/mTreg ratio was an independent predictor of unfavorable 90 day outcome in AIS. However, large sample-size cohort studies are needed to confirm our findings.https://www.frontiersin.org/articles/10.3389/fnagi.2023.1072980/fullacute ischemic strokeprognosisregulatory T cellsnaïve regulatory T cellsmemory regulatory T cells |
| spellingShingle | Gang Deng Yue Tang Jun Xiao Xin Chen Yun-Hui Chu Ke Shang Luo-Qi Zhou Chuan Qin Feng Wang Dai-Shi Tian Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke Frontiers in Aging Neuroscience acute ischemic stroke prognosis regulatory T cells naïve regulatory T cells memory regulatory T cells |
| title | Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke |
| title_full | Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke |
| title_fullStr | Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke |
| title_full_unstemmed | Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke |
| title_short | Naïve-memory regulatory T cells ratio is a prognostic biomarker for patients with acute ischemic stroke |
| title_sort | naive memory regulatory t cells ratio is a prognostic biomarker for patients with acute ischemic stroke |
| topic | acute ischemic stroke prognosis regulatory T cells naïve regulatory T cells memory regulatory T cells |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1072980/full |
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