Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD
OBJECTIVE: To validate and develop an immunonephelometric assay for the determination of alpha-1 antitrypsin (AAT) levels in dried blood spots from COPD patients in Brazil. METHODS: We determined AAT levels in serum samples and dried blood spots from 192 COPD patients. For the preparation of dr...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pneumologia e Tisiologia
2013-09-01
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| Series: | Jornal Brasileiro de Pneumologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000500547&lng=en&tlng=en |
| _version_ | 1818953732967104512 |
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| author | Laura Russo Zillmer Rodrigo Russo Beatriz Martins Manzano Ivan Ivanaga Oliver Augusto Nascimento Altay Alves Lino de Souza Gildo Santos Junior Francisco Rodriguez Marc Miravitlles Jose Roberto Jardim |
| author_facet | Laura Russo Zillmer Rodrigo Russo Beatriz Martins Manzano Ivan Ivanaga Oliver Augusto Nascimento Altay Alves Lino de Souza Gildo Santos Junior Francisco Rodriguez Marc Miravitlles Jose Roberto Jardim |
| author_sort | Laura Russo Zillmer |
| collection | DOAJ |
| description | OBJECTIVE: To validate and develop an immunonephelometric assay for the determination of alpha-1 antitrypsin (AAT) levels in dried blood spots from COPD patients in Brazil. METHODS: We determined AAT levels in serum samples and dried blood spots from 192 COPD patients. For the preparation of dried blood spots, a disk (diameter, 6 mm) was placed into a tube, eluted with 200 µL of PBS, and stored overnight at 4ºC. All of the samples were analyzed by immunonephelometry in duplicate. We used the bootstrap resampling method in order to determine a cut-off point for AAT levels in dried blood spots. RESULTS: The correlation coefficient between the AAT levels in serum samples and those in dried blood spots was r = 0.45. For dried blood spots, the cut-off value was 2.02 mg/dL (97% CI: 1.45-2.64 mg/dL), with a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 95.7%, 27.2%, and 100%, respectively. CONCLUSIONS: This method for the determination of AAT levels in dried blood spots appears to be a reliable screening tool for patients with AAT deficiency. |
| first_indexed | 2024-12-20T10:10:57Z |
| format | Article |
| id | doaj.art-454679f1ace2431d9a348c23533b1f9d |
| institution | Directory Open Access Journal |
| issn | 1806-3756 |
| language | English |
| last_indexed | 2024-12-20T10:10:57Z |
| publishDate | 2013-09-01 |
| publisher | Sociedade Brasileira de Pneumologia e Tisiologia |
| record_format | Article |
| series | Jornal Brasileiro de Pneumologia |
| spelling | doaj.art-454679f1ace2431d9a348c23533b1f9d2022-12-21T19:44:09ZengSociedade Brasileira de Pneumologia e TisiologiaJornal Brasileiro de Pneumologia1806-37562013-09-0139554755410.1590/S1806-37132013000500004S1806-37132013000500547Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPDLaura Russo ZillmerRodrigo RussoBeatriz Martins ManzanoIvan IvanagaOliver Augusto NascimentoAltay Alves Lino de SouzaGildo Santos JuniorFrancisco RodriguezMarc MiravitllesJose Roberto JardimOBJECTIVE: To validate and develop an immunonephelometric assay for the determination of alpha-1 antitrypsin (AAT) levels in dried blood spots from COPD patients in Brazil. METHODS: We determined AAT levels in serum samples and dried blood spots from 192 COPD patients. For the preparation of dried blood spots, a disk (diameter, 6 mm) was placed into a tube, eluted with 200 µL of PBS, and stored overnight at 4ºC. All of the samples were analyzed by immunonephelometry in duplicate. We used the bootstrap resampling method in order to determine a cut-off point for AAT levels in dried blood spots. RESULTS: The correlation coefficient between the AAT levels in serum samples and those in dried blood spots was r = 0.45. For dried blood spots, the cut-off value was 2.02 mg/dL (97% CI: 1.45-2.64 mg/dL), with a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 95.7%, 27.2%, and 100%, respectively. CONCLUSIONS: This method for the determination of AAT levels in dried blood spots appears to be a reliable screening tool for patients with AAT deficiency.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000500547&lng=en&tlng=enDeficiencia de alfa 1-antitripsinaNefelometria e turbidimetriaDoenca pulmonar obstrutiva cronica |
| spellingShingle | Laura Russo Zillmer Rodrigo Russo Beatriz Martins Manzano Ivan Ivanaga Oliver Augusto Nascimento Altay Alves Lino de Souza Gildo Santos Junior Francisco Rodriguez Marc Miravitlles Jose Roberto Jardim Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD Jornal Brasileiro de Pneumologia Deficiencia de alfa 1-antitripsina Nefelometria e turbidimetria Doenca pulmonar obstrutiva cronica |
| title | Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD |
| title_full | Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD |
| title_fullStr | Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD |
| title_full_unstemmed | Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD |
| title_short | Validation and development of an immunonephelometric assay for the determination of alpha-1 antitrypsin levels in dried blood spots from patients with COPD |
| title_sort | validation and development of an immunonephelometric assay for the determination of alpha 1 antitrypsin levels in dried blood spots from patients with copd |
| topic | Deficiencia de alfa 1-antitripsina Nefelometria e turbidimetria Doenca pulmonar obstrutiva cronica |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000500547&lng=en&tlng=en |
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