Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon
Objectives This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon. Methods A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes we...
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| Format: | Article |
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Korea Disease Control and Prevention Agency
2020-08-01
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| Series: | Osong Public Health and Research Perspectives |
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| Online Access: | http://ophrp.org/upload/pdf/ophrp-11-216.pdf |
| _version_ | 1797766088416559104 |
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| author | Wiliane J. T. Marbou Priyanka Jain Sriparna Samajpati Gourab Halder Asish K. Mukhopadhyay Shanta Dutta Victor Kuete |
| author_facet | Wiliane J. T. Marbou Priyanka Jain Sriparna Samajpati Gourab Halder Asish K. Mukhopadhyay Shanta Dutta Victor Kuete |
| author_sort | Wiliane J. T. Marbou |
| collection | DOAJ |
| description | Objectives This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon. Methods A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection. Results There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC. Conclusion These findings suggested that measures should be taken to reduce the harm of E. coli to public health. |
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| id | doaj.art-45473c2210244e46af5fe4c8364ebb56 |
| institution | Directory Open Access Journal |
| issn | 2210-9099 2210-9110 |
| language | English |
| last_indexed | 2024-03-12T20:20:19Z |
| publishDate | 2020-08-01 |
| publisher | Korea Disease Control and Prevention Agency |
| record_format | Article |
| series | Osong Public Health and Research Perspectives |
| spelling | doaj.art-45473c2210244e46af5fe4c8364ebb562023-08-02T00:59:43ZengKorea Disease Control and Prevention AgencyOsong Public Health and Research Perspectives2210-90992210-91102020-08-0111421623010.24171/j.phrp.2020.11.4.11570Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West CameroonWiliane J. T. Marbou0Priyanka Jain1Sriparna Samajpati2Gourab Halder3Asish K. Mukhopadhyay4Shanta Dutta5Victor Kuete6 Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon Bacteriology Division, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, India Bacteriology Division, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, India Bacteriology Division, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, India Bacteriology Division, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, India Bacteriology Division, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, India Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonObjectives This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon. Methods A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection. Results There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC. Conclusion These findings suggested that measures should be taken to reduce the harm of E. coli to public health.http://ophrp.org/upload/pdf/ophrp-11-216.pdfvirulenceantibiotics resistance |
| spellingShingle | Wiliane J. T. Marbou Priyanka Jain Sriparna Samajpati Gourab Halder Asish K. Mukhopadhyay Shanta Dutta Victor Kuete Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon Osong Public Health and Research Perspectives virulence antibiotics resistance |
| title | Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon |
| title_full | Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon |
| title_fullStr | Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon |
| title_full_unstemmed | Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon |
| title_short | Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon |
| title_sort | profiling virulence and antimicrobial resistance markers of enterovirulent escherichia coli from fecal isolates of adult patients with enteric infections in west cameroon |
| topic | virulence antibiotics resistance |
| url | http://ophrp.org/upload/pdf/ophrp-11-216.pdf |
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