Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages
In this study, <i>Mucuna pruriens</i> extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that <i>M. pruriens</i> extracted with hot water (MW), <i>M. pruriens<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8797 |
| _version_ | 1797456067714613248 |
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| author | Dong-Geun Han Min-Jun Bae Bong-Jeon An |
| author_facet | Dong-Geun Han Min-Jun Bae Bong-Jeon An |
| author_sort | Dong-Geun Han |
| collection | DOAJ |
| description | In this study, <i>Mucuna pruriens</i> extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that <i>M. pruriens</i> extracted with hot water (MW), <i>M. pruriens</i> extracted with 70% ethanol (ME), and <i>M. pruriens</i> extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in <i>M. pruriens</i> through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that <i>M. pruriens</i> extracts have the potential for use as an anti-inflammatory material. |
| first_indexed | 2024-03-09T16:02:08Z |
| format | Article |
| id | doaj.art-4549025f4cce49028b57521292934ec7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:02:08Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4549025f4cce49028b57521292934ec72023-11-24T16:57:37ZengMDPI AGMolecules1420-30492022-12-012724879710.3390/molecules27248797Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 MacrophagesDong-Geun Han0Min-Jun Bae1Bong-Jeon An2Department of Cosmeceutical Science, Daegu Hanny University, Gyeongsan 712715, Republic of KoreaDepartment of Cosmeceutical Science, Daegu Hanny University, Gyeongsan 712715, Republic of KoreaDepartment of Cosmeceutical Science, Daegu Hanny University, Gyeongsan 712715, Republic of KoreaIn this study, <i>Mucuna pruriens</i> extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that <i>M. pruriens</i> extracted with hot water (MW), <i>M. pruriens</i> extracted with 70% ethanol (ME), and <i>M. pruriens</i> extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in <i>M. pruriens</i> through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that <i>M. pruriens</i> extracts have the potential for use as an anti-inflammatory material.https://www.mdpi.com/1420-3049/27/24/8797<i>Mucuna pruriens</i>macrophagelipopolysaccharideanti-inflammatoryL-DOPA |
| spellingShingle | Dong-Geun Han Min-Jun Bae Bong-Jeon An Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages Molecules <i>Mucuna pruriens</i> macrophage lipopolysaccharide anti-inflammatory L-DOPA |
| title | Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages |
| title_full | Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages |
| title_fullStr | Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages |
| title_full_unstemmed | Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages |
| title_short | Anti-Inflammatory Activity of Velvet Bean (<i>Mucuna pruriens</i>) Substances in LPS−Stimulated RAW 264.7 Macrophages |
| title_sort | anti inflammatory activity of velvet bean i mucuna pruriens i substances in lps stimulated raw 264 7 macrophages |
| topic | <i>Mucuna pruriens</i> macrophage lipopolysaccharide anti-inflammatory L-DOPA |
| url | https://www.mdpi.com/1420-3049/27/24/8797 |
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