Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis
Abstract Background Through vernalization, plants achieve flowering competence by sensing prolonged cold exposure (constant exposure approximately 2-5 °C). During this process, plants initiate defense responses to endure cold conditions. Here, we conducted transcriptome analysis of Arabidopsis plant...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07763-3 |
| _version_ | 1819062516371685376 |
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| author | Fei Li Qian Hu Fadi Chen Jia Fu Jiang |
| author_facet | Fei Li Qian Hu Fadi Chen Jia Fu Jiang |
| author_sort | Fei Li |
| collection | DOAJ |
| description | Abstract Background Through vernalization, plants achieve flowering competence by sensing prolonged cold exposure (constant exposure approximately 2-5 °C). During this process, plants initiate defense responses to endure cold conditions. Here, we conducted transcriptome analysis of Arabidopsis plants subjected to prolonged cold exposure (6 weeks) to explore the physiological dynamics of vernalization and uncover the relationship between vernalization and cold stress. Results Time-lag initiation of the two pathways and weighted gene co-expression network analysis (WGCNA) revealed that vernalization is independent of cold acclimation. Moreover, WGCNA revealed three major networks involving ethylene and jasmonic acid response, cold acclimation, and chromatin modification in response to prolonged cold exposure. Finally, throughout vernalization, the cold stress response is regulated via an alternative splicing-mediated mechanism. Conclusion These findings illustrate a comprehensive picture of cold stress- and vernalization-mediated global changes in Arabidopsis. |
| first_indexed | 2024-12-21T15:00:01Z |
| format | Article |
| id | doaj.art-454afc6afe8d46d8bf82154ec2433724 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-21T15:00:01Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-454afc6afe8d46d8bf82154ec24337242022-12-21T18:59:37ZengBMCBMC Genomics1471-21642021-06-0122111410.1186/s12864-021-07763-3Transcriptome analysis reveals Vernalization is independent of cold acclimation in ArabidopsisFei Li0Qian Hu1Fadi Chen2Jia Fu Jiang3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural UniversityAbstract Background Through vernalization, plants achieve flowering competence by sensing prolonged cold exposure (constant exposure approximately 2-5 °C). During this process, plants initiate defense responses to endure cold conditions. Here, we conducted transcriptome analysis of Arabidopsis plants subjected to prolonged cold exposure (6 weeks) to explore the physiological dynamics of vernalization and uncover the relationship between vernalization and cold stress. Results Time-lag initiation of the two pathways and weighted gene co-expression network analysis (WGCNA) revealed that vernalization is independent of cold acclimation. Moreover, WGCNA revealed three major networks involving ethylene and jasmonic acid response, cold acclimation, and chromatin modification in response to prolonged cold exposure. Finally, throughout vernalization, the cold stress response is regulated via an alternative splicing-mediated mechanism. Conclusion These findings illustrate a comprehensive picture of cold stress- and vernalization-mediated global changes in Arabidopsis.https://doi.org/10.1186/s12864-021-07763-3ArabidopsisTranscriptome profilingCold stressVernalizationFLC |
| spellingShingle | Fei Li Qian Hu Fadi Chen Jia Fu Jiang Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis BMC Genomics Arabidopsis Transcriptome profiling Cold stress Vernalization FLC |
| title | Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis |
| title_full | Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis |
| title_fullStr | Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis |
| title_full_unstemmed | Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis |
| title_short | Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis |
| title_sort | transcriptome analysis reveals vernalization is independent of cold acclimation in arabidopsis |
| topic | Arabidopsis Transcriptome profiling Cold stress Vernalization FLC |
| url | https://doi.org/10.1186/s12864-021-07763-3 |
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