Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought
Drought is an environmental stressor that affects crop yield worldwide. Understanding plant physiological responses to stress conditions is needed to secure food in future climate conditions. In this study, we applied a combination of plant physiology and metabolomic techniques to understand plant r...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.652143/full |
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| author | Andres Echeverria Estíbaliz Larrainzar Weiqiang Li Weiqiang Li Weiqiang Li Yasuko Watanabe Muneo Sato Cuong Duy Tran Cuong Duy Tran Jose A. Moler Masami Yokota Hirai Yuji Sawada Lam-Son Phan Tran Lam-Son Phan Tran Lam-Son Phan Tran Esther M. Gonzalez |
| author_facet | Andres Echeverria Estíbaliz Larrainzar Weiqiang Li Weiqiang Li Weiqiang Li Yasuko Watanabe Muneo Sato Cuong Duy Tran Cuong Duy Tran Jose A. Moler Masami Yokota Hirai Yuji Sawada Lam-Son Phan Tran Lam-Son Phan Tran Lam-Son Phan Tran Esther M. Gonzalez |
| author_sort | Andres Echeverria |
| collection | DOAJ |
| description | Drought is an environmental stressor that affects crop yield worldwide. Understanding plant physiological responses to stress conditions is needed to secure food in future climate conditions. In this study, we applied a combination of plant physiology and metabolomic techniques to understand plant responses to progressive water deficit focusing on the root system. We chose two legume plants with contrasting tolerance to drought, the widely cultivated alfalfa Medicago sativa (Ms) and the model legume Medicago truncatula (Mt) for comparative analysis. Ms taproot (tapR) and Mt fibrous root (fibR) biomass increased during drought, while a progressive decline in water content was observed in both species. Metabolomic analysis allowed the identification of key metabolites in the different tissues tested. Under drought, carbohydrates, abscisic acid, and proline predominantly accumulated in leaves and tapRs, whereas flavonoids increased in fibRs in both species. Raffinose-family related metabolites accumulated during drought. Along with an accumulation of root sucrose in plants subjected to drought, both species showed a decrease in sucrose synthase (SUS) activity related to a reduction in the transcript level of SUS1, the main SUS gene. This study highlights the relevance of root carbon metabolism during drought conditions and provides evidence on the specific accumulation of metabolites throughout the root system. |
| first_indexed | 2024-12-21T20:02:23Z |
| format | Article |
| id | doaj.art-454d0ce12d2546f1b54be9d7915821cb |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T20:02:23Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-454d0ce12d2546f1b54be9d7915821cb2022-12-21T18:51:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.652143652143Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to DroughtAndres Echeverria0Estíbaliz Larrainzar1Weiqiang Li2Weiqiang Li3Weiqiang Li4Yasuko Watanabe5Muneo Sato6Cuong Duy Tran7Cuong Duy Tran8Jose A. Moler9Masami Yokota Hirai10Yuji Sawada11Lam-Son Phan Tran12Lam-Son Phan Tran13Lam-Son Phan Tran14Esther M. Gonzalez15Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra, Pamplona, SpainInstitute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra, Pamplona, SpainState Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, Kaifeng, ChinaHenan Joint International Laboratory for Crop Multi-Omics Research, Henan University, Kaifeng, ChinaStress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, JapanStress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, JapanMetabolic System Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, JapanStress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, JapanAgricultural Genetics Institute, Vietnam Academy of Agricultural Sciences, Hanoi, VietnamDepartment of Statistics, Computing and Mathematics, Public University of Navarra, Pamplona, SpainMetabolic System Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, JapanMetabolic System Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, JapanStress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, JapanInstitute of Research and Development, Duy Tan University, Da Nang, VietnamInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, United StatesInstitute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra, Pamplona, SpainDrought is an environmental stressor that affects crop yield worldwide. Understanding plant physiological responses to stress conditions is needed to secure food in future climate conditions. In this study, we applied a combination of plant physiology and metabolomic techniques to understand plant responses to progressive water deficit focusing on the root system. We chose two legume plants with contrasting tolerance to drought, the widely cultivated alfalfa Medicago sativa (Ms) and the model legume Medicago truncatula (Mt) for comparative analysis. Ms taproot (tapR) and Mt fibrous root (fibR) biomass increased during drought, while a progressive decline in water content was observed in both species. Metabolomic analysis allowed the identification of key metabolites in the different tissues tested. Under drought, carbohydrates, abscisic acid, and proline predominantly accumulated in leaves and tapRs, whereas flavonoids increased in fibRs in both species. Raffinose-family related metabolites accumulated during drought. Along with an accumulation of root sucrose in plants subjected to drought, both species showed a decrease in sucrose synthase (SUS) activity related to a reduction in the transcript level of SUS1, the main SUS gene. This study highlights the relevance of root carbon metabolism during drought conditions and provides evidence on the specific accumulation of metabolites throughout the root system.https://www.frontiersin.org/articles/10.3389/fpls.2021.652143/fullplant rootsdrought stresssucrosesucrose synthaseraffinoseflavonoids |
| spellingShingle | Andres Echeverria Estíbaliz Larrainzar Weiqiang Li Weiqiang Li Weiqiang Li Yasuko Watanabe Muneo Sato Cuong Duy Tran Cuong Duy Tran Jose A. Moler Masami Yokota Hirai Yuji Sawada Lam-Son Phan Tran Lam-Son Phan Tran Lam-Son Phan Tran Esther M. Gonzalez Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought Frontiers in Plant Science plant roots drought stress sucrose sucrose synthase raffinose flavonoids |
| title | Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought |
| title_full | Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought |
| title_fullStr | Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought |
| title_full_unstemmed | Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought |
| title_short | Medicago sativa and Medicago truncatula Show Contrasting Root Metabolic Responses to Drought |
| title_sort | medicago sativa and medicago truncatula show contrasting root metabolic responses to drought |
| topic | plant roots drought stress sucrose sucrose synthase raffinose flavonoids |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.652143/full |
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