Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro
Objective The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Asian-Australasian Association of Animal Production Societies
2020-01-01
|
| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://ajas.info/upload/pdf/ajas-19-0226.pdf |
| _version_ | 1819124028892250112 |
|---|---|
| author | Hua Zhang Jinjin Tong Zun Wang Benhai Xiong Linshu Jiang |
| author_facet | Hua Zhang Jinjin Tong Zun Wang Benhai Xiong Linshu Jiang |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | Objective The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. Results The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment significantly increased the proportion of propionate, while the acetate/propionate ratio was significantly decreased. At the genus level, the relative abundance of Methanomassiliicoccus was significantly increased, while the relative abundance of Methanobrevibacter decreased significantly in the GSPE group. Conclusion In conclusion, GSPE is a plant extract that can reduce methane production by affecting the structures of archaeal communities, which was achieved by a substitution of Methanobrevibacter with Methanomassiliicoccus. |
| first_indexed | 2024-12-22T07:17:44Z |
| format | Article |
| id | doaj.art-454e6d15763f4336bef595b2142b4d87 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-22T07:17:44Z |
| publishDate | 2020-01-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-454e6d15763f4336bef595b2142b4d872022-12-21T18:34:22ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172020-01-01331616810.5713/ajas.19.022624292Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitroHua Zhang0Jinjin Tong1Zun Wang2Benhai Xiong3Linshu Jiang4 Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, ChinaObjective The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. Results The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment significantly increased the proportion of propionate, while the acetate/propionate ratio was significantly decreased. At the genus level, the relative abundance of Methanomassiliicoccus was significantly increased, while the relative abundance of Methanobrevibacter decreased significantly in the GSPE group. Conclusion In conclusion, GSPE is a plant extract that can reduce methane production by affecting the structures of archaeal communities, which was achieved by a substitution of Methanobrevibacter with Methanomassiliicoccus.http://ajas.info/upload/pdf/ajas-19-0226.pdfgrape seed procyanidin extractarchaeal communitymethanogenesismiseq high-throughput sequencing |
| spellingShingle | Hua Zhang Jinjin Tong Zun Wang Benhai Xiong Linshu Jiang Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro Asian-Australasian Journal of Animal Sciences grape seed procyanidin extract archaeal community methanogenesis miseq high-throughput sequencing |
| title | Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro |
| title_full | Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro |
| title_fullStr | Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro |
| title_full_unstemmed | Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro |
| title_short | Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro |
| title_sort | illumina miseq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro |
| topic | grape seed procyanidin extract archaeal community methanogenesis miseq high-throughput sequencing |
| url | http://ajas.info/upload/pdf/ajas-19-0226.pdf |
| work_keys_str_mv | AT huazhang illuminamiseqsequencingrevealstheeffectsofgrapeseedprocyanidinonrumenarchaealcommunitiesinvitro AT jinjintong illuminamiseqsequencingrevealstheeffectsofgrapeseedprocyanidinonrumenarchaealcommunitiesinvitro AT zunwang illuminamiseqsequencingrevealstheeffectsofgrapeseedprocyanidinonrumenarchaealcommunitiesinvitro AT benhaixiong illuminamiseqsequencingrevealstheeffectsofgrapeseedprocyanidinonrumenarchaealcommunitiesinvitro AT linshujiang illuminamiseqsequencingrevealstheeffectsofgrapeseedprocyanidinonrumenarchaealcommunitiesinvitro |