Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro
The purpose of this study was to make an initial assessment of new PEG (polyethylene glycol)-functionalized C<sub>60</sub> fullerene derivative for potential bone tissue engineering applications. Thus, Fourier Transform Infrared spectroscopy analysis, thermogravimetric analysis, and cycl...
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MDPI AG
2021-03-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/6/1566 |
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| author | Piotr Piotrowski Katarzyna Klimek Grazyna Ginalska Andrzej Kaim |
| author_facet | Piotr Piotrowski Katarzyna Klimek Grazyna Ginalska Andrzej Kaim |
| author_sort | Piotr Piotrowski |
| collection | DOAJ |
| description | The purpose of this study was to make an initial assessment of new PEG (polyethylene glycol)-functionalized C<sub>60</sub> fullerene derivative for potential bone tissue engineering applications. Thus, Fourier Transform Infrared spectroscopy analysis, thermogravimetric analysis, and cyclic voltammetry measurement were performed. Moreover, cell culture experiments in vitro were carried out using normal human osteoblasts. Cell viability and proliferation were evaluated using colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test as well as by fluorescent staining. It was demonstrated that resultant derivative possessed good solubility in water, high temperature stability, and retained favorable electron accepting properties of C<sub>60</sub> fullerene core. Most important, new fullerene derivatives at low concentrations did not exhibit cytotoxic effect and supported osteoblast proliferation compared to control. Thanks to all mentioned properties of new PEG-functionalized C<sub>60</sub> fullerene derivative, it seems that it could be used as a component of polymer-based bone scaffolds in order to enhance their biological properties. |
| first_indexed | 2024-03-10T12:59:51Z |
| format | Article |
| id | doaj.art-45511ca990f5434bbea03cf304e1cddc |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T12:59:51Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-45511ca990f5434bbea03cf304e1cddc2023-11-21T11:35:03ZengMDPI AGMaterials1996-19442021-03-01146156610.3390/ma14061566Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In VitroPiotr Piotrowski0Katarzyna Klimek1Grazyna Ginalska2Andrzej Kaim3Department of Chemistry, University of Warsaw, Pasteura 1 Street, 02-093 Warsaw, PolandDepartment of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, PolandDepartment of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, PolandDepartment of Chemistry, University of Warsaw, Pasteura 1 Street, 02-093 Warsaw, PolandThe purpose of this study was to make an initial assessment of new PEG (polyethylene glycol)-functionalized C<sub>60</sub> fullerene derivative for potential bone tissue engineering applications. Thus, Fourier Transform Infrared spectroscopy analysis, thermogravimetric analysis, and cyclic voltammetry measurement were performed. Moreover, cell culture experiments in vitro were carried out using normal human osteoblasts. Cell viability and proliferation were evaluated using colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test as well as by fluorescent staining. It was demonstrated that resultant derivative possessed good solubility in water, high temperature stability, and retained favorable electron accepting properties of C<sub>60</sub> fullerene core. Most important, new fullerene derivatives at low concentrations did not exhibit cytotoxic effect and supported osteoblast proliferation compared to control. Thanks to all mentioned properties of new PEG-functionalized C<sub>60</sub> fullerene derivative, it seems that it could be used as a component of polymer-based bone scaffolds in order to enhance their biological properties.https://www.mdpi.com/1996-1944/14/6/1566functionalized fullerenesPEGwater solubilitycytotoxicitycell proliferationosteoblasts |
| spellingShingle | Piotr Piotrowski Katarzyna Klimek Grazyna Ginalska Andrzej Kaim Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro Materials functionalized fullerenes PEG water solubility cytotoxicity cell proliferation osteoblasts |
| title | Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro |
| title_full | Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro |
| title_fullStr | Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro |
| title_full_unstemmed | Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro |
| title_short | Beneficial Influence of Water-Soluble PEG-Functionalized C<sub>60</sub> Fullerene on Human Osteoblast Growth In Vitro |
| title_sort | beneficial influence of water soluble peg functionalized c sub 60 sub fullerene on human osteoblast growth in vitro |
| topic | functionalized fullerenes PEG water solubility cytotoxicity cell proliferation osteoblasts |
| url | https://www.mdpi.com/1996-1944/14/6/1566 |
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