Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig
Background To minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Thieme Medical Publishers, Inc.
2013-01-01
|
| Series: | Archives of Plastic Surgery |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2013.40.1.11 |
| _version_ | 1798041717579972608 |
|---|---|
| author | Hyunji Kim Daegu Son Tae Hyun Choi Samhyun Jung Sunyoung Kwon Junhyung Kim Kihwan Han |
| author_facet | Hyunji Kim Daegu Son Tae Hyun Choi Samhyun Jung Sunyoung Kwon Junhyung Kim Kihwan Han |
| author_sort | Hyunji Kim |
| collection | DOAJ |
| description | Background To minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified dermal substitutes were generated according to the cross-linking type.
Methods The AM-collagen dermal substitutes were characterized and compared with currently used dermal substitutes in a pig skin defect model. There were five experimental groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT), DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical), Terudermis, Integra, and AlloDerm. After 3×3 cm full-thickness skin defects on the back of a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the neodermis. The take rate of the dermal substitutes, skin, and histological sections were all assessed at 1, 2, and 4 weeks postoperatively.
Results More rapid healing and a higher take rate were evident in the AM-DHT and Terudermis groups. Histological examination revealed fewer inflammatory cells and more fibroblast hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed collagen was significantly more appropriate in the AM-DHT group.
Conclusions These observations provide supporting evidence that a newly developed amniotic-collagen dermal substitute may inhibit inflammatory reactions and promote wound healing. |
| first_indexed | 2024-04-11T22:25:26Z |
| format | Article |
| id | doaj.art-455c31bbae2143ab932aa456a48a859e |
| institution | Directory Open Access Journal |
| issn | 2234-6163 2234-6171 |
| language | English |
| last_indexed | 2024-04-11T22:25:26Z |
| publishDate | 2013-01-01 |
| publisher | Thieme Medical Publishers, Inc. |
| record_format | Article |
| series | Archives of Plastic Surgery |
| spelling | doaj.art-455c31bbae2143ab932aa456a48a859e2022-12-22T03:59:45ZengThieme Medical Publishers, Inc.Archives of Plastic Surgery2234-61632234-61712013-01-014001111810.5999/aps.2013.40.1.11Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a PigHyunji Kim0Daegu Son1Tae Hyun Choi2Samhyun Jung3Sunyoung Kwon4Junhyung Kim5Kihwan Han6Suibi Plastic Surgery Clinic, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, KoreaDepartment of Tissue Engineering, Bioland, Cheongwon, KoreaDepartment of Pathology, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, KoreaBackground To minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified dermal substitutes were generated according to the cross-linking type. Methods The AM-collagen dermal substitutes were characterized and compared with currently used dermal substitutes in a pig skin defect model. There were five experimental groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT), DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical), Terudermis, Integra, and AlloDerm. After 3×3 cm full-thickness skin defects on the back of a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the neodermis. The take rate of the dermal substitutes, skin, and histological sections were all assessed at 1, 2, and 4 weeks postoperatively. Results More rapid healing and a higher take rate were evident in the AM-DHT and Terudermis groups. Histological examination revealed fewer inflammatory cells and more fibroblast hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed collagen was significantly more appropriate in the AM-DHT group. Conclusions These observations provide supporting evidence that a newly developed amniotic-collagen dermal substitute may inhibit inflammatory reactions and promote wound healing.http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2013.40.1.11amniondermisbiologic dressingscollagenchondroitin sulfates |
| spellingShingle | Hyunji Kim Daegu Son Tae Hyun Choi Samhyun Jung Sunyoung Kwon Junhyung Kim Kihwan Han Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig Archives of Plastic Surgery amnion dermis biologic dressings collagen chondroitin sulfates |
| title | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_full | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_fullStr | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_full_unstemmed | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_short | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_sort | evaluation of an amniotic membrane collagen dermal substitute in the management of full thickness skin defects in a pig |
| topic | amnion dermis biologic dressings collagen chondroitin sulfates |
| url | http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2013.40.1.11 |
| work_keys_str_mv | AT hyunjikim evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig AT daeguson evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig AT taehyunchoi evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig AT samhyunjung evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig AT sunyoungkwon evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig AT junhyungkim evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig AT kihwanhan evaluationofanamnioticmembranecollagendermalsubstituteinthemanagementoffullthicknessskindefectsinapig |