The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype
The ideal rice phenotype is that of plants exhibiting fewer panicles with high biomass, large grain number, flag leaf area with small insertion angles, and an erected morphology improving light interception. The sunflower transcription factor HaHB11, homeodomain–leucine zipper I, confers increased s...
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MDPI AG
2023-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/5/826 |
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| author | Jesica Raineri Luciano Nicolás Caraballo Maximiliano Gómez Raquel Lía Chan |
| author_facet | Jesica Raineri Luciano Nicolás Caraballo Maximiliano Gómez Raquel Lía Chan |
| author_sort | Jesica Raineri |
| collection | DOAJ |
| description | The ideal rice phenotype is that of plants exhibiting fewer panicles with high biomass, large grain number, flag leaf area with small insertion angles, and an erected morphology improving light interception. The sunflower transcription factor HaHB11, homeodomain–leucine zipper I, confers increased seed yield and abiotic stress tolerance to Arabidopsis and maize. Here, we report the obtaining and characterization of rice plants expressing <i>HaHB11</i> driven by its promoter or the 35S constitutive one. Transgenic <i>p35S:HaHB11</i> plants closely resembled the ideal high-yield phenotype, whereas those carrying the <i>pHaHB11:HaHB11</i> construct were hard to distinguish from the wild type. The former had an erected architecture, enhanced vegetative leaf biomass, rolled flag leaves with a larger surface, sharper insertion angles insensitive to brassinosteroids, and higher harvest index and seed biomass than the wild type. The combination of the distinct features exhibited by <i>p35S:HaHB11</i> plants, including the increased number of set grains per panicle, supports the high-yield phenotype. We wondered where <i>HaHB11</i> has to be expressed to achieve the high-yield phenotype and evaluated <i>HaHB11</i> expression levels in all tissues. The results indicate that its expression is particularly necessary in the flag leaf and panicle to produce the ideal phenotype. |
| first_indexed | 2024-03-11T03:54:35Z |
| format | Article |
| id | doaj.art-455dca8d51084e2182bc3c4178e46cea |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T03:54:35Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj.art-455dca8d51084e2182bc3c4178e46cea2023-11-18T00:39:45ZengMDPI AGBiomolecules2218-273X2023-05-0113582610.3390/biom13050826The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal PhenotypeJesica Raineri0Luciano Nicolás Caraballo1Maximiliano Gómez2Raquel Lía Chan3Instituto de Agrobiotecnología del Litoral, CONICET, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, Santa Fe 3000, ArgentinaInstituto de Agrobiotecnología del Litoral, CONICET, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, Santa Fe 3000, ArgentinaInstituto de Agrobiotecnología del Litoral, CONICET, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, Santa Fe 3000, ArgentinaInstituto de Agrobiotecnología del Litoral, CONICET, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, Santa Fe 3000, ArgentinaThe ideal rice phenotype is that of plants exhibiting fewer panicles with high biomass, large grain number, flag leaf area with small insertion angles, and an erected morphology improving light interception. The sunflower transcription factor HaHB11, homeodomain–leucine zipper I, confers increased seed yield and abiotic stress tolerance to Arabidopsis and maize. Here, we report the obtaining and characterization of rice plants expressing <i>HaHB11</i> driven by its promoter or the 35S constitutive one. Transgenic <i>p35S:HaHB11</i> plants closely resembled the ideal high-yield phenotype, whereas those carrying the <i>pHaHB11:HaHB11</i> construct were hard to distinguish from the wild type. The former had an erected architecture, enhanced vegetative leaf biomass, rolled flag leaves with a larger surface, sharper insertion angles insensitive to brassinosteroids, and higher harvest index and seed biomass than the wild type. The combination of the distinct features exhibited by <i>p35S:HaHB11</i> plants, including the increased number of set grains per panicle, supports the high-yield phenotype. We wondered where <i>HaHB11</i> has to be expressed to achieve the high-yield phenotype and evaluated <i>HaHB11</i> expression levels in all tissues. The results indicate that its expression is particularly necessary in the flag leaf and panicle to produce the ideal phenotype.https://www.mdpi.com/2218-273X/13/5/826riceGMOHaHB11HD-Zip Iidiotypeseed production |
| spellingShingle | Jesica Raineri Luciano Nicolás Caraballo Maximiliano Gómez Raquel Lía Chan The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype Biomolecules rice GMO HaHB11 HD-Zip I idiotype seed production |
| title | The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype |
| title_full | The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype |
| title_fullStr | The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype |
| title_full_unstemmed | The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype |
| title_short | The Transcription Factor HaHB11 Boosts Grain Set and Yield in Rice Plants, Allowing Them to Approach Their Ideal Phenotype |
| title_sort | transcription factor hahb11 boosts grain set and yield in rice plants allowing them to approach their ideal phenotype |
| topic | rice GMO HaHB11 HD-Zip I idiotype seed production |
| url | https://www.mdpi.com/2218-273X/13/5/826 |
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