The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents
Ji Cheng Zhang, Jin Hua Wang, Jia Lin, Guo Ming Su, Yi Lin Shen, Qi Wei Guo, Jia Jing Cai, Jun Yi Liu, Ke Xin Jia, Ding Zhi Fang Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, People’s Republic of Ch...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-05-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
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| Online Access: | https://www.dovepress.com/the-waisthip-ratio-is-a-mediator-between-serum-levels-of-brain-derived-peer-reviewed-fulltext-article-DMSO |
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| author | Zhang JC Wang JH Lin J Su GM Shen YL Guo QW Cai JJ Liu JY Jia KX Fang DZ |
| author_facet | Zhang JC Wang JH Lin J Su GM Shen YL Guo QW Cai JJ Liu JY Jia KX Fang DZ |
| author_sort | Zhang JC |
| collection | DOAJ |
| description | Ji Cheng Zhang, Jin Hua Wang, Jia Lin, Guo Ming Su, Yi Lin Shen, Qi Wei Guo, Jia Jing Cai, Jun Yi Liu, Ke Xin Jia, Ding Zhi Fang Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Ding Zhi Fang, Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, People’s Republic of China, Email dzfang@scu.edu.cnAim: To explore anthropometric, metabolic and dietary factors associated with and their interplays with the Val66Met polymorphism at brain-derived neurotrophic factor (BDNF) gene (Bdnf) on serum BDNF levels in adolescents.Methods: Serum BDNF levels were quantified using an enzyme-linked immunosorbent assay in 644 high school students (278 males/366 females). A polymerase chain reaction and restriction fragment length polymorphism assay were utilized for Bdnf Val66Met genotyping followed by verification using DNA sequencing. Serum levels of metabolic characteristics were assayed by routine methods. The intake of macro and micronutrients was collected by a three-day food record.Results: Serum BDNF levels were found to be significantly different in the subjects with different genotypes of Bdnf Val66Met (Val/Val homozygotes, 60.05 ± 28.07 ng/mL vs Val/Met heterozygotes, 56.37 ± 29.34 ng/mL vs Met/Met homozygotes, 51.32 ± 24.54 ng/mL, p = 0.022). Among the 36 tested variables, waist–hip ratio (WHR) (β = − 0.163, p < 0.001), iodine intake (β = 0.132, p = 0.001), heart rate (β = 0.108, p = 0.005), high-density lipoprotein cholesterol (HDL-C) (β = 0.098, p = 0.011) and dietary fiber intake (β = 0.082, p = 0.084) were the predictor of serum BDNF levels, while SBP (β = 0.097, p = 0.013) and WHR (β = 0.091, p = 0.021) were related with Bdnf Val66Met. Moreover, WHR was observed to play a partial mediating role in the relationship between Bdnf Val66Met and serum BDNF levels (95% CI [− 1.161, − 0.087]) and contribute 13.05% of its total effect on serum BDNF levels.Conclusion: There are interplays between WHR and Bdnf Val66Met on serum BDNF levels, which may be among the explanations for the previous heterogeneous reports and provide novel insights into the regulation of serum BDNF levels.Keywords: serum, brain-derived neurotrophic factor, Val66Met, waist–hip ratio, mediator, adolescent |
| first_indexed | 2024-03-13T08:36:53Z |
| format | Article |
| id | doaj.art-455f76b665364990b171038f37d660af |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-03-13T08:36:53Z |
| publishDate | 2023-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-455f76b665364990b171038f37d660af2023-05-30T19:13:36ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072023-05-01Volume 161555156584073The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in AdolescentsZhang JCWang JHLin JSu GMShen YLGuo QWCai JJLiu JYJia KXFang DZJi Cheng Zhang, Jin Hua Wang, Jia Lin, Guo Ming Su, Yi Lin Shen, Qi Wei Guo, Jia Jing Cai, Jun Yi Liu, Ke Xin Jia, Ding Zhi Fang Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Ding Zhi Fang, Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, People’s Republic of China, Email dzfang@scu.edu.cnAim: To explore anthropometric, metabolic and dietary factors associated with and their interplays with the Val66Met polymorphism at brain-derived neurotrophic factor (BDNF) gene (Bdnf) on serum BDNF levels in adolescents.Methods: Serum BDNF levels were quantified using an enzyme-linked immunosorbent assay in 644 high school students (278 males/366 females). A polymerase chain reaction and restriction fragment length polymorphism assay were utilized for Bdnf Val66Met genotyping followed by verification using DNA sequencing. Serum levels of metabolic characteristics were assayed by routine methods. The intake of macro and micronutrients was collected by a three-day food record.Results: Serum BDNF levels were found to be significantly different in the subjects with different genotypes of Bdnf Val66Met (Val/Val homozygotes, 60.05 ± 28.07 ng/mL vs Val/Met heterozygotes, 56.37 ± 29.34 ng/mL vs Met/Met homozygotes, 51.32 ± 24.54 ng/mL, p = 0.022). Among the 36 tested variables, waist–hip ratio (WHR) (β = − 0.163, p < 0.001), iodine intake (β = 0.132, p = 0.001), heart rate (β = 0.108, p = 0.005), high-density lipoprotein cholesterol (HDL-C) (β = 0.098, p = 0.011) and dietary fiber intake (β = 0.082, p = 0.084) were the predictor of serum BDNF levels, while SBP (β = 0.097, p = 0.013) and WHR (β = 0.091, p = 0.021) were related with Bdnf Val66Met. Moreover, WHR was observed to play a partial mediating role in the relationship between Bdnf Val66Met and serum BDNF levels (95% CI [− 1.161, − 0.087]) and contribute 13.05% of its total effect on serum BDNF levels.Conclusion: There are interplays between WHR and Bdnf Val66Met on serum BDNF levels, which may be among the explanations for the previous heterogeneous reports and provide novel insights into the regulation of serum BDNF levels.Keywords: serum, brain-derived neurotrophic factor, Val66Met, waist–hip ratio, mediator, adolescenthttps://www.dovepress.com/the-waisthip-ratio-is-a-mediator-between-serum-levels-of-brain-derived-peer-reviewed-fulltext-article-DMSOserumbrain-derived neurotrophic factorval66metwaist-hip ratiomediatoradolescent |
| spellingShingle | Zhang JC Wang JH Lin J Su GM Shen YL Guo QW Cai JJ Liu JY Jia KX Fang DZ The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents Diabetes, Metabolic Syndrome and Obesity serum brain-derived neurotrophic factor val66met waist-hip ratio mediator adolescent |
| title | The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents |
| title_full | The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents |
| title_fullStr | The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents |
| title_full_unstemmed | The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents |
| title_short | The Waist–Hip Ratio is a Mediator Between Serum Levels of Brain-Derived Neurotrophic Factor and Its Val66Met Polymorphism in Adolescents |
| title_sort | waist ndash hip ratio is a mediator between serum levels of brain derived neurotrophic factor and its val66met polymorphism in adolescents |
| topic | serum brain-derived neurotrophic factor val66met waist-hip ratio mediator adolescent |
| url | https://www.dovepress.com/the-waisthip-ratio-is-a-mediator-between-serum-levels-of-brain-derived-peer-reviewed-fulltext-article-DMSO |
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