Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model
Cancer vaccines have always been a subject of gene therapy research. One of the most successful approaches has been working with genetically modified tumor cells. In this study, we describe our approach to achieving an immune response against a murine melanoma model, employing B16 tumor cells expres...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-10-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/4/11/1058 |
| _version_ | 1817994257861967872 |
|---|---|
| author | Salvador F. Aliño Maria Sánchez Rosa Algás Rafael Botella Luis Sendra Antonio Miguel María José Herrero |
| author_facet | Salvador F. Aliño Maria Sánchez Rosa Algás Rafael Botella Luis Sendra Antonio Miguel María José Herrero |
| author_sort | Salvador F. Aliño |
| collection | DOAJ |
| description | Cancer vaccines have always been a subject of gene therapy research. One of the most successful approaches has been working with genetically modified tumor cells. In this study, we describe our approach to achieving an immune response against a murine melanoma model, employing B16 tumor cells expressing GM-CSF and B7.2. Wild B16 cells were injected in C57BL6 mice to cause the tumor. Irradiated B16 cells transfected with GM-CSF, B7.2, or both, were processed as a preventive and therapeutic vaccination. Tumor volumes were measured and survival curves were obtained. Blood samples were taken from mice, and IgGs of each treatment group were also measured. The regulatory T cells (Treg) of selected groups were quantified using counts of images taken by confocal microscopy. Results: one hundred percent survival was achieved by preventive vaccination with the group of cells transfected with p2F_GM-CSF. Therapeutic vaccination achieved initial inhibition of tumor growth but did not secure overall survival of the animals. Classical Treg cells did not vary among the different groups in this therapeutic vaccination model. |
| first_indexed | 2024-04-14T01:50:38Z |
| format | Article |
| id | doaj.art-455fc3aeb918459480cb59a63c431e8d |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T01:50:38Z |
| publishDate | 2012-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-455fc3aeb918459480cb59a63c431e8d2022-12-22T02:19:22ZengMDPI AGToxins2072-66512012-10-014111058108110.3390/toxins4111058Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine ModelSalvador F. AliñoMaria SánchezRosa AlgásRafael BotellaLuis SendraAntonio MiguelMaría José HerreroCancer vaccines have always been a subject of gene therapy research. One of the most successful approaches has been working with genetically modified tumor cells. In this study, we describe our approach to achieving an immune response against a murine melanoma model, employing B16 tumor cells expressing GM-CSF and B7.2. Wild B16 cells were injected in C57BL6 mice to cause the tumor. Irradiated B16 cells transfected with GM-CSF, B7.2, or both, were processed as a preventive and therapeutic vaccination. Tumor volumes were measured and survival curves were obtained. Blood samples were taken from mice, and IgGs of each treatment group were also measured. The regulatory T cells (Treg) of selected groups were quantified using counts of images taken by confocal microscopy. Results: one hundred percent survival was achieved by preventive vaccination with the group of cells transfected with p2F_GM-CSF. Therapeutic vaccination achieved initial inhibition of tumor growth but did not secure overall survival of the animals. Classical Treg cells did not vary among the different groups in this therapeutic vaccination model.http://www.mdpi.com/2072-6651/4/11/1058cancer vaccinesgene therapynon-viralGM-CSFB7.2 |
| spellingShingle | Salvador F. Aliño Maria Sánchez Rosa Algás Rafael Botella Luis Sendra Antonio Miguel María José Herrero Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model Toxins cancer vaccines gene therapy non-viral GM-CSF B7.2 |
| title | Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model |
| title_full | Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model |
| title_fullStr | Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model |
| title_full_unstemmed | Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model |
| title_short | Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model |
| title_sort | comparative antitumor effect of preventive versus therapeutic vaccines employing b16 melanoma cells genetically modified to express gm csf and b7 2 in a murine model |
| topic | cancer vaccines gene therapy non-viral GM-CSF B7.2 |
| url | http://www.mdpi.com/2072-6651/4/11/1058 |
| work_keys_str_mv | AT salvadorfalino comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel AT mariasanchez comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel AT rosaalgas comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel AT rafaelbotella comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel AT luissendra comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel AT antoniomiguel comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel AT mariajoseherrero comparativeantitumoreffectofpreventiveversustherapeuticvaccinesemployingb16melanomacellsgeneticallymodifiedtoexpressgmcsfandb72inamurinemodel |