Correction of cyto-biochemical process disorders of cervical mucosa with cervical intraepithelial neoplasia
<b>Correction of cyto-biochemical process disorders of cervical mucosa with cervical intraepithelial neoplasia<br> </b><br> I.O. Borovikov, E.E. Gerasimenko, H.I. Gorring<br> Kuban State Medical University, Krasnodar, Russian Federation<br> <br> The art...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
LCC «Medicine-Inform»
2018-12-01
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| Series: | РМЖ. Мать и дитя |
| Online Access: | http://wchjournal.com/upload/iblock/9b7/9b7b5e62a2726c8d753da110389f15e5.pdf |
| Summary: | <b>Correction of cyto-biochemical process disorders of cervical mucosa with cervical intraepithelial neoplasia<br>
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I.O. Borovikov, E.E. Gerasimenko, H.I. Gorring<br>
Kuban State Medical University, Krasnodar, Russian Federation<br>
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The article describes the experience of using a topical form of bioflavonoid dihydroquercetin (Flamena<sup>®</sup>) in the correction of pro/antioxidant local disorders of cervical mucosa with a mild cervical intraepithelial neoplasia (CIN I).<br>
<b>Aim</b>: to evaluate the possibility of correcting disorders of oxidative processes in the cervical mucosa withcervical intraepithelial neoplasia using topical application of the drug dihydroquercetin (Flamena<sup>®</sup>).<br>
<b>Patients and Methods:</b> an electron paramagnetic resonance (EPR) method was used to study changes in the paramagnetic centers of the cervical mucosa in 64 women with mild cervical intraepithelial neoplasia (30 women — the control group). Free nitrogen oxide (NO), reactive forms of oxygen (superoxide radicals, O<sup>2–</sup>) and lipids (peroxide radicals, LOO–) were studied. The EPR spectra were recorded on an RE-1307 radio spectrometer (Russia). Cytochemistry of macrophages of the cervical mucosa (myeloperoxidase, acid phosphatase and nonspecific esterase) was studied in order to determine their activation under the conditions of the pathological process.<br>
<b>Results</b>: It was found that in the case of mild HPV-associated cervical neoplasia (CIN I), the intensity of the EPR signal of peroxide radicals (LOO–) increases by 278.9% (p<;0.05), and signals of superoxide radicals (O<sup>2–</sup>) are also recorded that are not detected in the group, an enhancement in the intensity of the EPR signal of NO increases by 233.3% compared with the control.<br>
<b>Conclusion</b>: thus, it has been proven that in the case of HPV infection in cervical tissue, there is an intensification of the formation of reactive forms of nitrogen and oxygen in epithelial cells, with changes in molecular, subcellular and cellular structures, which plays a significant role in the pathogenesis of the disease. These disorders can contribute to the malignancy of the affected cells and the initiation of the tumor process. During therapy with dihydroquercetin, the intensity of the EPR signal of spin-labeled peroxide radicals (LOO–) decreased 3.8 times, spin-labeled NO — 2.4 times, and superoxide radicals (O<sup>2–</sup>) practically stopped to be recorded.<br>
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<b>Key words:</b> cervical intraepithelial neoplasia, oxidative status, cervical mucosa, bioflavonoids, dihydroquercetin, Flamena.<br>
<b>For citation:</b> Borovikov I.O., Gerasimenko E.E., Gorring H.I. Correction of cyto-biochemical process disorders of cervical mucosa with cervical intraepithelial neoplasia. Russian Journal of Woman and Child Health. 2018;1(2):108–111.<br> |
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| ISSN: | 2618-8430 2686-7184 |