Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan
Abstract Background Reticuloendotheliosis virus (REV) is a gammaretrovirus that belongs to the family of Retroviridae. The infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia or T‐ and/ or B‐cell lymphoma, in a variety of domestic and wild bird...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.188 |
| _version_ | 1811306560789413888 |
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| author | Shima H. Alfaki Mohammed O. Hussien Fadwa M. Elsheikh Khalid M. Taha Atif H. Elbrissi Abdel Rahim M. El Hussein |
| author_facet | Shima H. Alfaki Mohammed O. Hussien Fadwa M. Elsheikh Khalid M. Taha Atif H. Elbrissi Abdel Rahim M. El Hussein |
| author_sort | Shima H. Alfaki |
| collection | DOAJ |
| description | Abstract Background Reticuloendotheliosis virus (REV) is a gammaretrovirus that belongs to the family of Retroviridae. The infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia or T‐ and/ or B‐cell lymphoma, in a variety of domestic and wild birds. The disease is widespread around the world. No related data have been reported in Sudan about the disease. The present study was conducted to determine the prevalence of REV antibodies and DNA in local and commercial breeds of chickens older than 20 weeks from June 2014 to February, 2017. Methods A total of 460 sera samples and 150 (50 liver and 100 spleen) tissue samples were collected from local and commercial breeds of chickens older than 20 weeks and screened for anti‐REV antibodies in four states of Sudan using a commercial REV antibody ELISA test kit (IDEXX). Polymerase chain reaction (PCR) was performed to detect REV DNA in tissue samples in Khartoum State. Results The results revealed that the overall seroprevalence of REV was 74.6% among local and commercial chicken breeds, but in commercial it was 79.5% (190/239) and 69.2% in local breeds (153/221). One hundred and fifty tissue samples of chickens (50 liver, 100 spleen) were tested using PCR for detection of REV using primer sets of the conserved region in envelope glycoprotein (env) gene with a band length of 850 bp. Five out of 50 (10%) liver samples were RE provirus DNA positive detected by PCR, whereas 15 out of 100 (15%) spleen samples were PCR positive. Univariate analysis revealed there was a difference (p ≤ 0.05) between locality and breed of chickens and seropositivity to REV. Conclusions The prevalence of the disease was high in Sudan and more studies are needed to evaluate the epidemiology and pathogenesis of the virus. |
| first_indexed | 2024-04-13T08:47:39Z |
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| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-13T08:47:39Z |
| publishDate | 2019-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-455fcd2d09f34ce98862b9a41391af712022-12-22T02:53:37ZengWileyVeterinary Medicine and Science2053-10952019-11-015450851110.1002/vms3.188Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in SudanShima H. Alfaki0Mohammed O. Hussien1Fadwa M. Elsheikh2Khalid M. Taha3Atif H. Elbrissi4Abdel Rahim M. El Hussein5Central Veterinary Research Laboratory (CVRL) Animal Resources Research Corporation (ARRC) Khartoum SudanCentral Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanCentral Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanAtbara Veterinary Research Laboratory (AVRL) Atbara SudanCentral Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanCentral Veterinary Research Laboratory (CVRL) Animal Resources Research Corporation (ARRC) Khartoum SudanAbstract Background Reticuloendotheliosis virus (REV) is a gammaretrovirus that belongs to the family of Retroviridae. The infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia or T‐ and/ or B‐cell lymphoma, in a variety of domestic and wild birds. The disease is widespread around the world. No related data have been reported in Sudan about the disease. The present study was conducted to determine the prevalence of REV antibodies and DNA in local and commercial breeds of chickens older than 20 weeks from June 2014 to February, 2017. Methods A total of 460 sera samples and 150 (50 liver and 100 spleen) tissue samples were collected from local and commercial breeds of chickens older than 20 weeks and screened for anti‐REV antibodies in four states of Sudan using a commercial REV antibody ELISA test kit (IDEXX). Polymerase chain reaction (PCR) was performed to detect REV DNA in tissue samples in Khartoum State. Results The results revealed that the overall seroprevalence of REV was 74.6% among local and commercial chicken breeds, but in commercial it was 79.5% (190/239) and 69.2% in local breeds (153/221). One hundred and fifty tissue samples of chickens (50 liver, 100 spleen) were tested using PCR for detection of REV using primer sets of the conserved region in envelope glycoprotein (env) gene with a band length of 850 bp. Five out of 50 (10%) liver samples were RE provirus DNA positive detected by PCR, whereas 15 out of 100 (15%) spleen samples were PCR positive. Univariate analysis revealed there was a difference (p ≤ 0.05) between locality and breed of chickens and seropositivity to REV. Conclusions The prevalence of the disease was high in Sudan and more studies are needed to evaluate the epidemiology and pathogenesis of the virus.https://doi.org/10.1002/vms3.188chickensELISAPCRprevalenceReticuloendotheliosis virus (REV)Sudan |
| spellingShingle | Shima H. Alfaki Mohammed O. Hussien Fadwa M. Elsheikh Khalid M. Taha Atif H. Elbrissi Abdel Rahim M. El Hussein Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan Veterinary Medicine and Science chickens ELISA PCR prevalence Reticuloendotheliosis virus (REV) Sudan |
| title | Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan |
| title_full | Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan |
| title_fullStr | Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan |
| title_full_unstemmed | Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan |
| title_short | Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan |
| title_sort | serological and molecular identification of reticuloendotheliosis virus rev in chickens in sudan |
| topic | chickens ELISA PCR prevalence Reticuloendotheliosis virus (REV) Sudan |
| url | https://doi.org/10.1002/vms3.188 |
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