Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells
Infrared spectroscopy has drawn considerable interest in biological applications, but the measurement of live cells is impeded by the attenuation of infrared light in water. Metasurface-enhanced infrared reflection spectroscopy (MEIRS) had been shown to mitigate the problem, enhance the cellular inf...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/10/1600 |
| _version_ | 1797500900420354048 |
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| author | Po-Ting Shen Steven H. Huang Zhouyang Huang Justin J. Wilson Gennady Shvets |
| author_facet | Po-Ting Shen Steven H. Huang Zhouyang Huang Justin J. Wilson Gennady Shvets |
| author_sort | Po-Ting Shen |
| collection | DOAJ |
| description | Infrared spectroscopy has drawn considerable interest in biological applications, but the measurement of live cells is impeded by the attenuation of infrared light in water. Metasurface-enhanced infrared reflection spectroscopy (MEIRS) had been shown to mitigate the problem, enhance the cellular infrared signal through surface-enhanced infrared absorption, and encode the cellular vibrational signatures in the reflectance spectrum at the same time. In this study, we used MEIRS to study the dynamic response of live cancer cells to a newly developed chemotherapeutic metal complex with distinct modes of action (MoAs): tricarbonyl rhenium isonitrile polypyridyl (TRIP). MEIRS measurements demonstrated that administering TRIP resulted in long-term (several hours) reduction in protein, lipid, and overall refractive index signals, and in short-term (tens of minutes) increase in these signals, consistent with the induction of endoplasmic reticulum stress. The unique tricarbonyl IR signature of TRIP in the bioorthogonal spectral window was monitored in real time, and was used as an infrared tag to detect the precise drug delivery time that was shown to be closely correlated with the onset of the phenotypic response. These results demonstrate that MEIRS is an effective label-free real-time cellular assay capable of detecting and interpreting the early phenotypic responses of cells to IR-tagged chemotherapeutics. |
| first_indexed | 2024-03-10T03:10:31Z |
| format | Article |
| id | doaj.art-45603a92460041b4a134b0297199a59a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:10:31Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-45603a92460041b4a134b0297199a59a2023-11-23T10:26:58ZengMDPI AGCells2073-44092022-05-011110160010.3390/cells11101600Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live CellsPo-Ting Shen0Steven H. Huang1Zhouyang Huang2Justin J. Wilson3Gennady Shvets4School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USASchool of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USADepartment of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USADepartment of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USASchool of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USAInfrared spectroscopy has drawn considerable interest in biological applications, but the measurement of live cells is impeded by the attenuation of infrared light in water. Metasurface-enhanced infrared reflection spectroscopy (MEIRS) had been shown to mitigate the problem, enhance the cellular infrared signal through surface-enhanced infrared absorption, and encode the cellular vibrational signatures in the reflectance spectrum at the same time. In this study, we used MEIRS to study the dynamic response of live cancer cells to a newly developed chemotherapeutic metal complex with distinct modes of action (MoAs): tricarbonyl rhenium isonitrile polypyridyl (TRIP). MEIRS measurements demonstrated that administering TRIP resulted in long-term (several hours) reduction in protein, lipid, and overall refractive index signals, and in short-term (tens of minutes) increase in these signals, consistent with the induction of endoplasmic reticulum stress. The unique tricarbonyl IR signature of TRIP in the bioorthogonal spectral window was monitored in real time, and was used as an infrared tag to detect the precise drug delivery time that was shown to be closely correlated with the onset of the phenotypic response. These results demonstrate that MEIRS is an effective label-free real-time cellular assay capable of detecting and interpreting the early phenotypic responses of cells to IR-tagged chemotherapeutics.https://www.mdpi.com/2073-4409/11/10/1600FTIRmetasurfacebiosensinglabel-freein vitrocancer cells |
| spellingShingle | Po-Ting Shen Steven H. Huang Zhouyang Huang Justin J. Wilson Gennady Shvets Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells Cells FTIR metasurface biosensing label-free in vitro cancer cells |
| title | Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells |
| title_full | Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells |
| title_fullStr | Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells |
| title_full_unstemmed | Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells |
| title_short | Probing the Drug Dynamics of Chemotherapeutics Using Metasurface-Enhanced Infrared Reflection Spectroscopy of Live Cells |
| title_sort | probing the drug dynamics of chemotherapeutics using metasurface enhanced infrared reflection spectroscopy of live cells |
| topic | FTIR metasurface biosensing label-free in vitro cancer cells |
| url | https://www.mdpi.com/2073-4409/11/10/1600 |
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