Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model
The management of urethral stricture remains a major therapeutic challenge in clinics. Herein, we explored the feasibility of reconstructing a relatively long segment of the urethra by the cell-seeded acellular artery in a canine model. The acellular arterial matrix was obtained from the excised car...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/8854479 |
| _version_ | 1798031732131233792 |
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| author | Hao Zhong Yamei Shen Danhui Zhao Guoqiang Yan Chengai Wu Guanglin Huang Zhizhong Liu Jianpo Zhai Qin Han |
| author_facet | Hao Zhong Yamei Shen Danhui Zhao Guoqiang Yan Chengai Wu Guanglin Huang Zhizhong Liu Jianpo Zhai Qin Han |
| author_sort | Hao Zhong |
| collection | DOAJ |
| description | The management of urethral stricture remains a major therapeutic challenge in clinics. Herein, we explored the feasibility of reconstructing a relatively long segment of the urethra by the cell-seeded acellular artery in a canine model. The acellular arterial matrix was obtained from the excised carotid artery of donor dogs. Autologous adipose-derived stem cells (ADSCs) from 6 male dogs were grown and seeded onto the premade acellular arterial matrix. A 3 cm long segment of the urethra was resected in 12 male dogs. Urethroplasty was performed with the acellular arterial matrix seeded with ADSCs in 6 animals and without cells in 6. Serial urethrography was performed at 1 and 3 months postoperatively. Wide urethral calibers without any signs of strictures were confirmed in all 6 animals in the experimental group. In contrast, urethral stricture was demonstrated in 3 animals in the control group. The graft was highly epithelialized and smooth in the experimental group, while graft contracture and scar formation were showed in the control group. Histologic analysis of the cell-seeded arterial matrix at 1 month confirmed the presence of multilayered urothelium and muscle. The levels of tissue formation developed over time with a progressive increase in muscle content. In contrast, extensive fibrosis and sparse smooth muscle were seen in animals treated with matrix without ADSCs. This study provides preclinical evidence that the ADSC-seeded arterial matrix can be used as a tubularized scaffold in the reconstruction of 3 cm long urethral defect in a male canine model. The ADSC-seeded arterial matrix remodels and regenerates normal-appearing urethral tissue layers over time. |
| first_indexed | 2024-04-11T20:02:19Z |
| format | Article |
| id | doaj.art-45715f9257a045fca28830acc32bc49d |
| institution | Directory Open Access Journal |
| issn | 1687-966X 1687-9678 |
| language | English |
| last_indexed | 2024-04-11T20:02:19Z |
| publishDate | 2021-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj.art-45715f9257a045fca28830acc32bc49d2022-12-22T04:05:32ZengHindawi LimitedStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/88544798854479Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine ModelHao Zhong0Yamei Shen1Danhui Zhao2Guoqiang Yan3Chengai Wu4Guanglin Huang5Zhizhong Liu6Jianpo Zhai7Qin Han8Department of Urology, Inner Mongolia Baogang Hospital, Baotou 014010, ChinaInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing Key Laboratory (No.BZO381), Beijing 100010, ChinaBeijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, ChinaBeijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, ChinaBeijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, ChinaDepartment of Urology, Beijing Jishuitan Hospital, Beijing 100096, ChinaDepartment of Urology, Inner Mongolia Baogang Hospital, Baotou 014010, ChinaDepartment of Urology, Beijing Jishuitan Hospital, Beijing 100096, ChinaInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing Key Laboratory (No.BZO381), Beijing 100010, ChinaThe management of urethral stricture remains a major therapeutic challenge in clinics. Herein, we explored the feasibility of reconstructing a relatively long segment of the urethra by the cell-seeded acellular artery in a canine model. The acellular arterial matrix was obtained from the excised carotid artery of donor dogs. Autologous adipose-derived stem cells (ADSCs) from 6 male dogs were grown and seeded onto the premade acellular arterial matrix. A 3 cm long segment of the urethra was resected in 12 male dogs. Urethroplasty was performed with the acellular arterial matrix seeded with ADSCs in 6 animals and without cells in 6. Serial urethrography was performed at 1 and 3 months postoperatively. Wide urethral calibers without any signs of strictures were confirmed in all 6 animals in the experimental group. In contrast, urethral stricture was demonstrated in 3 animals in the control group. The graft was highly epithelialized and smooth in the experimental group, while graft contracture and scar formation were showed in the control group. Histologic analysis of the cell-seeded arterial matrix at 1 month confirmed the presence of multilayered urothelium and muscle. The levels of tissue formation developed over time with a progressive increase in muscle content. In contrast, extensive fibrosis and sparse smooth muscle were seen in animals treated with matrix without ADSCs. This study provides preclinical evidence that the ADSC-seeded arterial matrix can be used as a tubularized scaffold in the reconstruction of 3 cm long urethral defect in a male canine model. The ADSC-seeded arterial matrix remodels and regenerates normal-appearing urethral tissue layers over time.http://dx.doi.org/10.1155/2021/8854479 |
| spellingShingle | Hao Zhong Yamei Shen Danhui Zhao Guoqiang Yan Chengai Wu Guanglin Huang Zhizhong Liu Jianpo Zhai Qin Han Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model Stem Cells International |
| title | Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model |
| title_full | Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model |
| title_fullStr | Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model |
| title_full_unstemmed | Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model |
| title_short | Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model |
| title_sort | cell seeded acellular artery for reconstruction of long urethral defects in a canine model |
| url | http://dx.doi.org/10.1155/2021/8854479 |
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