Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia
BackgroundPrimary aldosteronism (PA) is characterized by several metabolic changes such as insulin resistance, metabolic syndrome, and adipose tissue (AT) inflammation. Mi(cro)RNAs (miRNAs) are a class of non-coding small RNA molecules known to be critical regulators in several cellular processes as...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.930959/full |
| _version_ | 1828396441992691712 |
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| author | Antonio Concistrè Luigi Petramala Francesco Circosta Priscilla Romagnoli Maurizio Soldini Marco Bucci Domenico De Cesare Giuseppe Cavallaro Giorgio De Toma Francesco Cipollone Claudio Letizia |
| author_facet | Antonio Concistrè Luigi Petramala Francesco Circosta Priscilla Romagnoli Maurizio Soldini Marco Bucci Domenico De Cesare Giuseppe Cavallaro Giorgio De Toma Francesco Cipollone Claudio Letizia |
| author_sort | Antonio Concistrè |
| collection | DOAJ |
| description | BackgroundPrimary aldosteronism (PA) is characterized by several metabolic changes such as insulin resistance, metabolic syndrome, and adipose tissue (AT) inflammation. Mi(cro)RNAs (miRNAs) are a class of non-coding small RNA molecules known to be critical regulators in several cellular processes associated with AT dysfunction. The aim of this study was to evaluate the expression of some miRNAs in visceral and subcutaneous AT in patients undergoing adrenalectomy for aldosterone-secreting adrenal adenoma (APA) compared to the samples of AT obtained in patients undergoing adrenalectomy for non-functioning adrenal mass (NFA).MethodsThe quantitative expression of selected miRNA using real-time PCR was analyzed in surrounding adrenal neoplasia, peri-renal, and subcutaneous AT samples of 16 patients with adrenalectomy (11 patients with APA and 5 patients with NFA).ResultsReal-time PCR cycles for miRNA-132, miRNA-143, and miRNA-221 in fat surrounding adrenal neoplasia and in peri-adrenal AT were significantly higher in APA than in patients with NFA. Unlike patients with NFA, miRNA-132, miRNA-143, miRNA-221, and miRNA-26b were less expressed in surrounding adrenal neoplasia AT compared to subcutaneous AT in patients with APA.ConclusionThis study, conducted on tissue expression of miRNAs, highlights the possible pathophysiological role of some miRNAs in determining the metabolic alterations in patients with PA. |
| first_indexed | 2024-12-10T08:30:01Z |
| format | Article |
| id | doaj.art-4571a1e7710c4ec4a7dd9c2011c8e573 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-12-10T08:30:01Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-4571a1e7710c4ec4a7dd9c2011c8e5732022-12-22T01:56:07ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-07-01910.3389/fcvm.2022.930959930959Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasiaAntonio Concistrè0Luigi Petramala1Francesco Circosta2Priscilla Romagnoli3Maurizio Soldini4Marco Bucci5Domenico De Cesare6Giuseppe Cavallaro7Giorgio De Toma8Francesco Cipollone9Claudio Letizia10Department of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, “Sapienza” University of Rome, Rome, ItalyDepartment of Translational and Precision Medicine, “Sapienza” University of Rome, Rome, ItalyDepartment of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, “Sapienza” University of Rome, Rome, ItalyDepartment of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, “Sapienza” University of Rome, Rome, ItalyDepartment of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, “Sapienza” University of Rome, Rome, ItalyDepartment of Medicine and Aging Sciences, University “Gabriele d'Annunzio” of Chieti-Pescara, Chieti, ItalyDepartment of Medicine and Aging Sciences, University “Gabriele d'Annunzio” of Chieti-Pescara, Chieti, ItalyDepartment of Surgery “Pietro Valdoni, ” “Sapienza” University of Rome, Rome, ItalyDepartment of Surgery “Pietro Valdoni, ” “Sapienza” University of Rome, Rome, ItalyDepartment of Medicine and Aging Sciences, University “Gabriele d'Annunzio” of Chieti-Pescara, Chieti, ItalyDepartment of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, “Sapienza” University of Rome, Rome, ItalyBackgroundPrimary aldosteronism (PA) is characterized by several metabolic changes such as insulin resistance, metabolic syndrome, and adipose tissue (AT) inflammation. Mi(cro)RNAs (miRNAs) are a class of non-coding small RNA molecules known to be critical regulators in several cellular processes associated with AT dysfunction. The aim of this study was to evaluate the expression of some miRNAs in visceral and subcutaneous AT in patients undergoing adrenalectomy for aldosterone-secreting adrenal adenoma (APA) compared to the samples of AT obtained in patients undergoing adrenalectomy for non-functioning adrenal mass (NFA).MethodsThe quantitative expression of selected miRNA using real-time PCR was analyzed in surrounding adrenal neoplasia, peri-renal, and subcutaneous AT samples of 16 patients with adrenalectomy (11 patients with APA and 5 patients with NFA).ResultsReal-time PCR cycles for miRNA-132, miRNA-143, and miRNA-221 in fat surrounding adrenal neoplasia and in peri-adrenal AT were significantly higher in APA than in patients with NFA. Unlike patients with NFA, miRNA-132, miRNA-143, miRNA-221, and miRNA-26b were less expressed in surrounding adrenal neoplasia AT compared to subcutaneous AT in patients with APA.ConclusionThis study, conducted on tissue expression of miRNAs, highlights the possible pathophysiological role of some miRNAs in determining the metabolic alterations in patients with PA.https://www.frontiersin.org/articles/10.3389/fcvm.2022.930959/fullmiRNAsprimary aldosteronismaldosterone-secreting adrenal adenomaadipose tissueadrenalectomy |
| spellingShingle | Antonio Concistrè Luigi Petramala Francesco Circosta Priscilla Romagnoli Maurizio Soldini Marco Bucci Domenico De Cesare Giuseppe Cavallaro Giorgio De Toma Francesco Cipollone Claudio Letizia Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia Frontiers in Cardiovascular Medicine miRNAs primary aldosteronism aldosterone-secreting adrenal adenoma adipose tissue adrenalectomy |
| title | Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia |
| title_full | Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia |
| title_fullStr | Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia |
| title_full_unstemmed | Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia |
| title_short | Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia |
| title_sort | analysis of the mirna expression from the adipose tissue surrounding the adrenal neoplasia |
| topic | miRNAs primary aldosteronism aldosterone-secreting adrenal adenoma adipose tissue adrenalectomy |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.930959/full |
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