Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice
Multiple studies show increased severity of SARS-CoV2-infection in patients with comorbidities such as hypertension and diabetes. In this study, we have prepared two herbal-based formulations, a pleiotropic herbal drink (Jin Si Herbal Tea, JHT) and a nasal drop (Jin Si nasal drop, JND), to provide p...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.953438/full |
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| author | Chien-Yi Chiang Chien-Yi Chiang Wei-Wen Kuo Wei-Wen Kuo Yu-Jung Lin Yu-Jung Lin Chia-Hua Kuo Cheng-Yen Shih Cheng-Yen Shih Pi-Yu Lin Pi-Yu Lin Shinn-Zong Lin Shinn-Zong Lin Shinn-Zong Lin Tsung-Jung Ho Tsung-Jung Ho Tsung-Jung Ho Tsung-Jung Ho Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Marthandam Asokan Shibu Marthandam Asokan Shibu |
| author_facet | Chien-Yi Chiang Chien-Yi Chiang Wei-Wen Kuo Wei-Wen Kuo Yu-Jung Lin Yu-Jung Lin Chia-Hua Kuo Cheng-Yen Shih Cheng-Yen Shih Pi-Yu Lin Pi-Yu Lin Shinn-Zong Lin Shinn-Zong Lin Shinn-Zong Lin Tsung-Jung Ho Tsung-Jung Ho Tsung-Jung Ho Tsung-Jung Ho Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Marthandam Asokan Shibu Marthandam Asokan Shibu |
| author_sort | Chien-Yi Chiang |
| collection | DOAJ |
| description | Multiple studies show increased severity of SARS-CoV2-infection in patients with comorbidities such as hypertension and diabetes. In this study, we have prepared two herbal-based formulations, a pleiotropic herbal drink (Jin Si Herbal Tea, JHT) and a nasal drop (Jin Si nasal drop, JND), to provide preventive care against SARS-CoV2 infection. The effect of JHT and JND was determined in SARS-CoV2-S-pseudotyped lentivirus-infected bronchial and colorectal cell lines and in SKH-1 mouse models. For preliminary studies, ACE2 receptor abundant bronchial (Calu-3) and colorectal cells (Caco-2) were used to determine the effect of JHT and JND on the host entry of various variants of SARS-CoV2-S-pseudotyped lentivirus. A series of experiments were performed to understand the infection rate in SKH-1 mice (6 weeks old, n = 9), find the effective dosage of JHT and JND, and determine the combination effect of JHT and JND on the entry and adhesion of various variant SARS-CoV2-S-pseudotyped lentiviruses, which included highly transmissible delta and gamma mutants. Furthermore, the effect of combined JHT and JND was determined on diabetes-induced SKH-1 mice against the comorbidity-associated intense viral entry and accumulation. In addition, the effect of combined JHT and JND administration on viral transmission from infected SKH-1 mice to uninfected cage mate mice was determined. The results showed that both JHT and JND were effective in alleviating the viral entry and accumulation in the thorax and the abdominal area. While JHT showed a dose-dependent decrease in the viral load, JND showed early inhibition of viral entry from day 1 of the infection. Combined administration of 48.66 mg of JHT and 20 µL of JND showed rapid reduction in the viral entry and reduced the viral load (97–99%) in the infected mice within 3 days of treatment. Moreover, 16.22 mg of JHT and 20 µL JND reduced the viral infection in STZ-induced diabetic SKH-1 mice. Interestingly, combined JHT and JND also inhibited viral transmission among cage mates. The results, therefore, showed that combined administration of JHT and JND is a novel and an efficient strategy to potentially prevent SARS-CoV2 infection. |
| first_indexed | 2024-04-11T08:12:19Z |
| format | Article |
| id | doaj.art-457639ac7d64493bbbdea9e96961fc59 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T08:12:19Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-457639ac7d64493bbbdea9e96961fc592022-12-22T04:35:18ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-11-011310.3389/fphar.2022.953438953438Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 miceChien-Yi Chiang0Chien-Yi Chiang1Wei-Wen Kuo2Wei-Wen Kuo3Yu-Jung Lin4Yu-Jung Lin5Chia-Hua Kuo6Cheng-Yen Shih7Cheng-Yen Shih8Pi-Yu Lin9Pi-Yu Lin10Shinn-Zong Lin11Shinn-Zong Lin12Shinn-Zong Lin13Tsung-Jung Ho14Tsung-Jung Ho15Tsung-Jung Ho16Tsung-Jung Ho17Chih-Yang Huang18Chih-Yang Huang19Chih-Yang Huang20Chih-Yang Huang21Chih-Yang Huang22Chih-Yang Huang23Marthandam Asokan Shibu24Marthandam Asokan Shibu25Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, TaiwanJing Si Herbal Research and Application Center, Hualien, TaiwanDepartment of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, TaiwanPh.D. Program for Biotechnology Industry, China Medical University, Taichung, TaiwanCardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, TaiwanJing Si Herbal Research and Application Center, Hualien, TaiwanLaboratory of Exercise Biochemistry, University of Taipei, Taipei, TaiwanJing Si Herbal Research and Application Center, Hualien, TaiwanBuddhist Tzu Chi Charity Foundation, Hualien, TaiwanJing Si Herbal Research and Application Center, Hualien, TaiwanBuddhist Tzu Chi Charity Foundation, Hualien, TaiwanJing Si Herbal Research and Application Center, Hualien, TaiwanBuddhist Tzu Chi Bioinnovation Center, Buddhist Tzu Chi Medical Foundation, Hualien, TaiwanDepartment of Neurosurgery, Hualien Tzu Chi Hospital, Hualien, TaiwanJing Si Herbal Research and Application Center, Hualien, TaiwanDepartment of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan0Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, Taiwan1School of Post-Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Hualien, TaiwanCardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, TaiwanJing Si Herbal Research and Application Center, Hualien, Taiwan2Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan3Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan4Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan5Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, TaiwanJing Si Herbal Research and Application Center, Hualien, Taiwan6Department of Biotechnology, Bharathiar University, Coimbatore, IndiaMultiple studies show increased severity of SARS-CoV2-infection in patients with comorbidities such as hypertension and diabetes. In this study, we have prepared two herbal-based formulations, a pleiotropic herbal drink (Jin Si Herbal Tea, JHT) and a nasal drop (Jin Si nasal drop, JND), to provide preventive care against SARS-CoV2 infection. The effect of JHT and JND was determined in SARS-CoV2-S-pseudotyped lentivirus-infected bronchial and colorectal cell lines and in SKH-1 mouse models. For preliminary studies, ACE2 receptor abundant bronchial (Calu-3) and colorectal cells (Caco-2) were used to determine the effect of JHT and JND on the host entry of various variants of SARS-CoV2-S-pseudotyped lentivirus. A series of experiments were performed to understand the infection rate in SKH-1 mice (6 weeks old, n = 9), find the effective dosage of JHT and JND, and determine the combination effect of JHT and JND on the entry and adhesion of various variant SARS-CoV2-S-pseudotyped lentiviruses, which included highly transmissible delta and gamma mutants. Furthermore, the effect of combined JHT and JND was determined on diabetes-induced SKH-1 mice against the comorbidity-associated intense viral entry and accumulation. In addition, the effect of combined JHT and JND administration on viral transmission from infected SKH-1 mice to uninfected cage mate mice was determined. The results showed that both JHT and JND were effective in alleviating the viral entry and accumulation in the thorax and the abdominal area. While JHT showed a dose-dependent decrease in the viral load, JND showed early inhibition of viral entry from day 1 of the infection. Combined administration of 48.66 mg of JHT and 20 µL of JND showed rapid reduction in the viral entry and reduced the viral load (97–99%) in the infected mice within 3 days of treatment. Moreover, 16.22 mg of JHT and 20 µL JND reduced the viral infection in STZ-induced diabetic SKH-1 mice. Interestingly, combined JHT and JND also inhibited viral transmission among cage mates. The results, therefore, showed that combined administration of JHT and JND is a novel and an efficient strategy to potentially prevent SARS-CoV2 infection.https://www.frontiersin.org/articles/10.3389/fphar.2022.953438/fullherbal formulaSARS-CoV2 variantsdiabetesviral transmissionphytotherapyArtemisia argyi |
| spellingShingle | Chien-Yi Chiang Chien-Yi Chiang Wei-Wen Kuo Wei-Wen Kuo Yu-Jung Lin Yu-Jung Lin Chia-Hua Kuo Cheng-Yen Shih Cheng-Yen Shih Pi-Yu Lin Pi-Yu Lin Shinn-Zong Lin Shinn-Zong Lin Shinn-Zong Lin Tsung-Jung Ho Tsung-Jung Ho Tsung-Jung Ho Tsung-Jung Ho Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Chih-Yang Huang Marthandam Asokan Shibu Marthandam Asokan Shibu Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice Frontiers in Pharmacology herbal formula SARS-CoV2 variants diabetes viral transmission phytotherapy Artemisia argyi |
| title | Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice |
| title_full | Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice |
| title_fullStr | Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice |
| title_full_unstemmed | Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice |
| title_short | Combined effect of traditional Chinese herbal-based formulations Jing Si herbal tea and Jing Si nasal drop inhibits adhesion and transmission of SARS-CoV2 in diabetic SKH-1 mice |
| title_sort | combined effect of traditional chinese herbal based formulations jing si herbal tea and jing si nasal drop inhibits adhesion and transmission of sars cov2 in diabetic skh 1 mice |
| topic | herbal formula SARS-CoV2 variants diabetes viral transmission phytotherapy Artemisia argyi |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.953438/full |
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