Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide
Post anesthesia shivering which happens in some patients during recovery time after general anesthesia is followed by central hypothermia and peripheral vasoconstriction. In this study, the effect of opioidergic/nitrergic systems were determined on post anesthesia shivering in rat. Animals were cool...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2019-03-01
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| Series: | Acta Medica Iranica |
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| Online Access: | http://acta.tums.ac.ir/index.php/acta/article/view/7086 |
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| author | Alireza Mazarei Mojtaba Nasimi Nastaran Rahimi Mehdi Sanatkar Sina Tofigh Amirhossein Orandi Ahmad R. Dehpour |
| author_facet | Alireza Mazarei Mojtaba Nasimi Nastaran Rahimi Mehdi Sanatkar Sina Tofigh Amirhossein Orandi Ahmad R. Dehpour |
| author_sort | Alireza Mazarei |
| collection | DOAJ |
| description | Post anesthesia shivering which happens in some patients during recovery time after general anesthesia is followed by central hypothermia and peripheral vasoconstriction. In this study, the effect of opioidergic/nitrergic systems were determined on post anesthesia shivering in rat. Animals were cooled gently on a cold surface with indirect contact with a mixture of ice and water. Animals were treated with saline; methadone (a full opioid agonist, 10 mg/kg); naltrexone (an opioid receptor antagonist, 10 mg/kg); L-NAME (a nonselective nitric oxide synthase (NOS) inhibitor, 10 mg/kg). The core body temperature and the frequency of basal state- and post anesthetic-shivering were recorded using a stainless steel rectal probe (MLT-1403, AD Instruments®) and electromyography (EMG) electrodes connected to Animal Bio Amp (FE136, AD Instruments®) signal conditioner, respectively. Methadone administration reduced the frequency of shivering after anesthesia, while injection of naltrexone and L-NAME increased post anesthetic shivering compared to vehicle group. Co-administration of L-NAME and methadone showed a significant decrease the frequency of post anesthetic shivering. Furthermore, the temperature of shivering onset was reduced following methadone administration, which was blocked by injection of both naltrexone and/or L-NAME. To conclude, the findings of this study revealed the protective impact of methadone on post anesthesia shivering-induced with hypothermia dominated to nitrergic pathway effects in rat. |
| first_indexed | 2024-04-12T04:57:51Z |
| format | Article |
| id | doaj.art-45764fde7f944a9bbd79b9eb49b1e079 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-04-12T04:57:51Z |
| publishDate | 2019-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-45764fde7f944a9bbd79b9eb49b1e0792022-12-22T03:47:03ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942019-03-011101157086Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric OxideAlireza Mazarei0Mojtaba Nasimi1Nastaran Rahimi2Mehdi Sanatkar3Sina Tofigh4Amirhossein Orandi5Ahmad R. Dehpour6a Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. b Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.a Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. b Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.a Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. b Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anesthesiology and Critical Care, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.a Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. b Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anesthesiology, School of Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Post anesthesia shivering which happens in some patients during recovery time after general anesthesia is followed by central hypothermia and peripheral vasoconstriction. In this study, the effect of opioidergic/nitrergic systems were determined on post anesthesia shivering in rat. Animals were cooled gently on a cold surface with indirect contact with a mixture of ice and water. Animals were treated with saline; methadone (a full opioid agonist, 10 mg/kg); naltrexone (an opioid receptor antagonist, 10 mg/kg); L-NAME (a nonselective nitric oxide synthase (NOS) inhibitor, 10 mg/kg). The core body temperature and the frequency of basal state- and post anesthetic-shivering were recorded using a stainless steel rectal probe (MLT-1403, AD Instruments®) and electromyography (EMG) electrodes connected to Animal Bio Amp (FE136, AD Instruments®) signal conditioner, respectively. Methadone administration reduced the frequency of shivering after anesthesia, while injection of naltrexone and L-NAME increased post anesthetic shivering compared to vehicle group. Co-administration of L-NAME and methadone showed a significant decrease the frequency of post anesthetic shivering. Furthermore, the temperature of shivering onset was reduced following methadone administration, which was blocked by injection of both naltrexone and/or L-NAME. To conclude, the findings of this study revealed the protective impact of methadone on post anesthesia shivering-induced with hypothermia dominated to nitrergic pathway effects in rat.http://acta.tums.ac.ir/index.php/acta/article/view/7086ShiveringHypothermiaMethadoneNitric oxideRat |
| spellingShingle | Alireza Mazarei Mojtaba Nasimi Nastaran Rahimi Mehdi Sanatkar Sina Tofigh Amirhossein Orandi Ahmad R. Dehpour Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide Acta Medica Iranica Shivering Hypothermia Methadone Nitric oxide Rat |
| title | Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide |
| title_full | Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide |
| title_fullStr | Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide |
| title_full_unstemmed | Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide |
| title_short | Methadone’s Effect on Hypothermia-Induced Shivering in Post Anesthetic Rat: Role of Nitric Oxide |
| title_sort | methadone s effect on hypothermia induced shivering in post anesthetic rat role of nitric oxide |
| topic | Shivering Hypothermia Methadone Nitric oxide Rat |
| url | http://acta.tums.ac.ir/index.php/acta/article/view/7086 |
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