Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements

Objectives: To investigate whether live birth (LB) is associated with oocyte yield and number of biopsied and suitable blastocyst to transfer following preimplantation genetic testing (PGT) for monogenic disorders (PGT-M) and chromosomal structural rearrangements (PGT-SR). Study design: All couples...

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Main Authors: Jara Ben-Nagi, Benjamin Jones, Roy Naja, Ahmed Amer, Sesh Sunkara, Sioban SenGupta, Paul Serhal
Format: Article
Language:English
Published: Elsevier 2019-10-01
Series:European Journal of Obstetrics & Gynecology and Reproductive Biology: X
Online Access:http://www.sciencedirect.com/science/article/pii/S2590161319300894
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author Jara Ben-Nagi
Benjamin Jones
Roy Naja
Ahmed Amer
Sesh Sunkara
Sioban SenGupta
Paul Serhal
author_facet Jara Ben-Nagi
Benjamin Jones
Roy Naja
Ahmed Amer
Sesh Sunkara
Sioban SenGupta
Paul Serhal
author_sort Jara Ben-Nagi
collection DOAJ
description Objectives: To investigate whether live birth (LB) is associated with oocyte yield and number of biopsied and suitable blastocyst to transfer following preimplantation genetic testing (PGT) for monogenic disorders (PGT-M) and chromosomal structural rearrangements (PGT-SR). Study design: All couples underwent controlled ovarian stimulation, blastocyst biopsy, vitrification and transfer of suitable embryo(s) in a frozen embryo transfer (FET) cycle. Results: Of 175 couples who underwent PGT treatment, 249 oocytes retrievals were carried out and 230 FET were subsequently undertaken. 122/230 (53%, 95% CI 47–59) FET resulted in a LB and 16/230 (7%, 95% CI 4–11) have resulted in ongoing pregnancies. 21/230 (9%, 95% CI 6–14) FET resulted in miscarriage and 69/230 (30%, 95% CI 24–36) concluded with failed implantation. Two (1%, 95% CI 0–3) transfers underwent termination for congenital malformation, with no evidence of misdiagnosis by prenatal testing. The relationship between number of oocytes retrieved and number of blastocysts biopsied and suitable embryos to transfer were significant (p = 0.00; Incidence rate ratio (IRR) 1.05; 95% 1.04–1.06; p = 0.00; IRR 1.04; 95%, 1.03–1.06), respectively. The number of oocytes collected (p = 0.007; OR 1.06; 95% CI 1.01–1.10), the number of blastocysts biopsied (p = 0.001; OR 1.14; 95% 95% CI 1.06–1.23) and the number of suitable embryos to transfer (p = 0.00; OR 1.38; 95% CI 1.17–1.64) were all significantly associated with the odds of achieving a LB. There is a 14% and 38% increased chance of a LB per additional blastocyst biopsied and suitable embryo to transfer, respectively. Conclusions: PGT-M and PGT-SR outcomes are significantly associated with egg yield, number of blastocysts to biopsy and suitable embryos to transfer. Keywords: Live birth, Preimplantation genetic testing, Monogenic disorders, Chromosomal rearrangements, Oocyte, Blastocysts
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spelling doaj.art-4579863c125e414b867d859ac221ce112022-12-21T18:54:59ZengElsevierEuropean Journal of Obstetrics & Gynecology and Reproductive Biology: X2590-16132019-10-014Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangementsJara Ben-Nagi0Benjamin Jones1Roy Naja2Ahmed Amer3Sesh Sunkara4Sioban SenGupta5Paul Serhal6Centre for Reproductive and Genetic Health, 230–232 Great Portland Street, London, W1W 5QS, UK; Corresponding author.Imperial College London, Du Cane Road, London, W12 0NN, UKIGENOMIX, 40 Occam Road, Guildford, Surrey, GU2 7YG, UKCentre for Reproductive and Genetic Health, 230–232 Great Portland Street, London, W1W 5QS, UKQueen’s Hospital, Barking Havering and Redbridge University Hospitals NHS Trust, Essex, UKUCL, Institute for Women’s Health, 86-96 Chenies Mews, London, WC1E 6HX, UKCentre for Reproductive and Genetic Health, 230–232 Great Portland Street, London, W1W 5QS, UKObjectives: To investigate whether live birth (LB) is associated with oocyte yield and number of biopsied and suitable blastocyst to transfer following preimplantation genetic testing (PGT) for monogenic disorders (PGT-M) and chromosomal structural rearrangements (PGT-SR). Study design: All couples underwent controlled ovarian stimulation, blastocyst biopsy, vitrification and transfer of suitable embryo(s) in a frozen embryo transfer (FET) cycle. Results: Of 175 couples who underwent PGT treatment, 249 oocytes retrievals were carried out and 230 FET were subsequently undertaken. 122/230 (53%, 95% CI 47–59) FET resulted in a LB and 16/230 (7%, 95% CI 4–11) have resulted in ongoing pregnancies. 21/230 (9%, 95% CI 6–14) FET resulted in miscarriage and 69/230 (30%, 95% CI 24–36) concluded with failed implantation. Two (1%, 95% CI 0–3) transfers underwent termination for congenital malformation, with no evidence of misdiagnosis by prenatal testing. The relationship between number of oocytes retrieved and number of blastocysts biopsied and suitable embryos to transfer were significant (p = 0.00; Incidence rate ratio (IRR) 1.05; 95% 1.04–1.06; p = 0.00; IRR 1.04; 95%, 1.03–1.06), respectively. The number of oocytes collected (p = 0.007; OR 1.06; 95% CI 1.01–1.10), the number of blastocysts biopsied (p = 0.001; OR 1.14; 95% 95% CI 1.06–1.23) and the number of suitable embryos to transfer (p = 0.00; OR 1.38; 95% CI 1.17–1.64) were all significantly associated with the odds of achieving a LB. There is a 14% and 38% increased chance of a LB per additional blastocyst biopsied and suitable embryo to transfer, respectively. Conclusions: PGT-M and PGT-SR outcomes are significantly associated with egg yield, number of blastocysts to biopsy and suitable embryos to transfer. Keywords: Live birth, Preimplantation genetic testing, Monogenic disorders, Chromosomal rearrangements, Oocyte, Blastocystshttp://www.sciencedirect.com/science/article/pii/S2590161319300894
spellingShingle Jara Ben-Nagi
Benjamin Jones
Roy Naja
Ahmed Amer
Sesh Sunkara
Sioban SenGupta
Paul Serhal
Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements
European Journal of Obstetrics & Gynecology and Reproductive Biology: X
title Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements
title_full Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements
title_fullStr Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements
title_full_unstemmed Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements
title_short Live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing (PGT) cycles for monogenic disorders and chromosomal structural rearrangements
title_sort live birth rate is associated with oocyte yield and number of biopsied and suitable blastocysts to transfer in preimplantation genetic testing pgt cycles for monogenic disorders and chromosomal structural rearrangements
url http://www.sciencedirect.com/science/article/pii/S2590161319300894
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