CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis
The aim of this study was to evaluate the expression of chemokine receptor CXCR4 and its ligand CXCL12 in patients with gout and uric acid-induced inflammation. A total of 40 patients with intercritical gout and 27 controls were consecutively enrolled. The serum levels of interleukin-1β (IL-1β), IL-...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/3/649 |
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| author | Seong-Kyu Kim Jung-Yoon Choe Ki-Yeun Park |
| author_facet | Seong-Kyu Kim Jung-Yoon Choe Ki-Yeun Park |
| author_sort | Seong-Kyu Kim |
| collection | DOAJ |
| description | The aim of this study was to evaluate the expression of chemokine receptor CXCR4 and its ligand CXCL12 in patients with gout and uric acid-induced inflammation. A total of 40 patients with intercritical gout and 27 controls were consecutively enrolled. The serum levels of interleukin-1β (IL-1β), IL-18, CXCL12, and CXCR4 were assessed using an enzyme-linked immunosorbent assay. The gene and protein expressions for these target molecules were measured in human U937 cells incubated with monosodium urate (MSU) crystals using a real-time reverse transcription polymerase chain reaction and Western blot analysis. Patients with intercritical gout showed higher serum IL-1β, IL-18, and CXCL12 levels, but not the serum CXCR4 level, than in the controls.The serum CXCR4 level in gout patients was associated with the serum IL-18 level, uric acid level, and uric acid/creatinine ratio (<i>r</i> = 0.331, <i>p</i> = 0.037; <i>r</i> = 0.346, <i>p</i> = 0.028; and <i>r</i> = 0.361, <i>p</i> = 0.022, respectively). U937 cells treated with MSU crystals significantly induced the CXCL12 and CXCR4 mRNA and protein expression in addition to IL-1β and IL-18. In cells transfected with IL-1β siRNA or IL-18 siRNA, the CXCL12 and CXCR4 expression was downregulated compared with the non-transfected cells in MSU crystal-induced inflammation. In this study, we revealed that CXCL12 and CXCR4 were involved in the pathogenesis of uric acid-induced inflammation and gouty arthritis. |
| first_indexed | 2024-03-11T06:54:23Z |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T06:54:23Z |
| publishDate | 2023-02-01 |
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| series | Biomedicines |
| spelling | doaj.art-457dc326a91746d0bc01b6e97e5504092023-11-17T09:43:34ZengMDPI AGBiomedicines2227-90592023-02-0111364910.3390/biomedicines11030649CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty ArthritisSeong-Kyu Kim0Jung-Yoon Choe1Ki-Yeun Park2Division of Rheumatology, Department of Internal Medicine, Catholic University of Daegu School of Medicine, 33, Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Republic of KoreaDivision of Rheumatology, Department of Internal Medicine, Catholic University of Daegu School of Medicine, 33, Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Republic of KoreaArthritis and Autoimmunity Research Center, Catholic University of Daegu, 33, Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Republic of KoreaThe aim of this study was to evaluate the expression of chemokine receptor CXCR4 and its ligand CXCL12 in patients with gout and uric acid-induced inflammation. A total of 40 patients with intercritical gout and 27 controls were consecutively enrolled. The serum levels of interleukin-1β (IL-1β), IL-18, CXCL12, and CXCR4 were assessed using an enzyme-linked immunosorbent assay. The gene and protein expressions for these target molecules were measured in human U937 cells incubated with monosodium urate (MSU) crystals using a real-time reverse transcription polymerase chain reaction and Western blot analysis. Patients with intercritical gout showed higher serum IL-1β, IL-18, and CXCL12 levels, but not the serum CXCR4 level, than in the controls.The serum CXCR4 level in gout patients was associated with the serum IL-18 level, uric acid level, and uric acid/creatinine ratio (<i>r</i> = 0.331, <i>p</i> = 0.037; <i>r</i> = 0.346, <i>p</i> = 0.028; and <i>r</i> = 0.361, <i>p</i> = 0.022, respectively). U937 cells treated with MSU crystals significantly induced the CXCL12 and CXCR4 mRNA and protein expression in addition to IL-1β and IL-18. In cells transfected with IL-1β siRNA or IL-18 siRNA, the CXCL12 and CXCR4 expression was downregulated compared with the non-transfected cells in MSU crystal-induced inflammation. In this study, we revealed that CXCL12 and CXCR4 were involved in the pathogenesis of uric acid-induced inflammation and gouty arthritis.https://www.mdpi.com/2227-9059/11/3/649goutCXCL12CXCR4uric acid |
| spellingShingle | Seong-Kyu Kim Jung-Yoon Choe Ki-Yeun Park CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis Biomedicines gout CXCL12 CXCR4 uric acid |
| title | CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis |
| title_full | CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis |
| title_fullStr | CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis |
| title_full_unstemmed | CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis |
| title_short | CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis |
| title_sort | cxcl12 and cxcr4 as novel biomarkers in uric acid induced inflammation and patients with gouty arthritis |
| topic | gout CXCL12 CXCR4 uric acid |
| url | https://www.mdpi.com/2227-9059/11/3/649 |
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