Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients
Abstract Background Tuberculosis (TB) is a leading cause of morbidity and mortality worldwide. Control of TB is lingering by the lack of diagnostic tests that are simple, rapid, yet accurate. Thus, smear-negative pulmonary TB often misses the diagnosis. The study evaluated the performance of GeneXpe...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-022-07287-5 |
| _version_ | 1811284719750348800 |
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| author | Raksha Rimal Dhiraj Shrestha Susil Pyakurel Rashmi Poudel Prasha Shrestha Kul Raj Rai Gokarna Raj Ghimire Ganesh Rai Shiba Kumar Rai |
| author_facet | Raksha Rimal Dhiraj Shrestha Susil Pyakurel Rashmi Poudel Prasha Shrestha Kul Raj Rai Gokarna Raj Ghimire Ganesh Rai Shiba Kumar Rai |
| author_sort | Raksha Rimal |
| collection | DOAJ |
| description | Abstract Background Tuberculosis (TB) is a leading cause of morbidity and mortality worldwide. Control of TB is lingering by the lack of diagnostic tests that are simple, rapid, yet accurate. Thus, smear-negative pulmonary TB often misses the diagnosis. The study evaluated the performance of GeneXpert MTB/RIF assay for the detection of Mycobacterium tuberculosis (MTB). Methods The study was carried out from June to December 2016 in Nepal Tuberculosis Center, Bhaktapur, Nepal. A total of 173 sputum samples were collected and processed by microscopy [Auramine-O staining and Ziehl–Neelsen (ZN) staining], followed by GeneXpert MTB/RIF assay and culture in Lowenstein-Jensen (LJ) medium. Results Of 173 sputum samples, 162 (93.6%) were smear-negative. Of 162 smear-negative sputum samples, 35 (21.6%) were confirmed to have MTB by culture, and 31 (19.1%) by GeneXpert MTB/RIF assay. Of 31 GeneXpert-positive samples, 25 (80.6%) were susceptible, 4 (12.9%) were resistant, and 2 (6.45%) were intermediate to rifampicin. The sensitivity, specificity, positive predictive value, and negative predictive value of GeneXpert MTB/RIF assay for smear-negative sputum samples were 74.3%, 96.6%, 86.7%, and 92%, respectively. The GeneXpert MTB/RIF has a substantial diagnostic agreement of 90.91% with culture (Cohen’s Kappa coefficient = 0.73). Conclusion The diagnostic performance of GeneXpert MTB/RIF assay was almost on par with culture, and thus can be relied upon for MTB detection in smear-negative sputum samples. |
| first_indexed | 2024-04-13T02:33:51Z |
| format | Article |
| id | doaj.art-457f34ef97864d4298b82437d35e1ef5 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T02:33:51Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-457f34ef97864d4298b82437d35e1ef52022-12-22T03:06:27ZengBMCBMC Infectious Diseases1471-23342022-04-012211710.1186/s12879-022-07287-5Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patientsRaksha Rimal0Dhiraj Shrestha1Susil Pyakurel2Rashmi Poudel3Prasha Shrestha4Kul Raj Rai5Gokarna Raj Ghimire6Ganesh Rai7Shiba Kumar Rai8Department of Microbiology, Shi-Gan International College of Science and TechnologyDepartment of Microbiology, Shi-Gan International College of Science and TechnologyDepartment of Microbiology, Shi-Gan International College of Science and TechnologyDepartment of Microbiology, Shi-Gan International College of Science and TechnologyKey Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry UniversityKey Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry UniversityNational Tuberculosis CenterDepartment of Microbiology, Shi-Gan International College of Science and TechnologyDepartment of Microbiology, Nepal Medical College and Teaching HospitalAbstract Background Tuberculosis (TB) is a leading cause of morbidity and mortality worldwide. Control of TB is lingering by the lack of diagnostic tests that are simple, rapid, yet accurate. Thus, smear-negative pulmonary TB often misses the diagnosis. The study evaluated the performance of GeneXpert MTB/RIF assay for the detection of Mycobacterium tuberculosis (MTB). Methods The study was carried out from June to December 2016 in Nepal Tuberculosis Center, Bhaktapur, Nepal. A total of 173 sputum samples were collected and processed by microscopy [Auramine-O staining and Ziehl–Neelsen (ZN) staining], followed by GeneXpert MTB/RIF assay and culture in Lowenstein-Jensen (LJ) medium. Results Of 173 sputum samples, 162 (93.6%) were smear-negative. Of 162 smear-negative sputum samples, 35 (21.6%) were confirmed to have MTB by culture, and 31 (19.1%) by GeneXpert MTB/RIF assay. Of 31 GeneXpert-positive samples, 25 (80.6%) were susceptible, 4 (12.9%) were resistant, and 2 (6.45%) were intermediate to rifampicin. The sensitivity, specificity, positive predictive value, and negative predictive value of GeneXpert MTB/RIF assay for smear-negative sputum samples were 74.3%, 96.6%, 86.7%, and 92%, respectively. The GeneXpert MTB/RIF has a substantial diagnostic agreement of 90.91% with culture (Cohen’s Kappa coefficient = 0.73). Conclusion The diagnostic performance of GeneXpert MTB/RIF assay was almost on par with culture, and thus can be relied upon for MTB detection in smear-negative sputum samples.https://doi.org/10.1186/s12879-022-07287-5GeneXpert MTB/RIFMTBMycobacterium tuberculosisNepalSmear-negative |
| spellingShingle | Raksha Rimal Dhiraj Shrestha Susil Pyakurel Rashmi Poudel Prasha Shrestha Kul Raj Rai Gokarna Raj Ghimire Ganesh Rai Shiba Kumar Rai Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients BMC Infectious Diseases GeneXpert MTB/RIF MTB Mycobacterium tuberculosis Nepal Smear-negative |
| title | Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients |
| title_full | Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients |
| title_fullStr | Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients |
| title_full_unstemmed | Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients |
| title_short | Diagnostic performance of GeneXpert MTB/RIF in detecting MTB in smear-negative presumptive TB patients |
| title_sort | diagnostic performance of genexpert mtb rif in detecting mtb in smear negative presumptive tb patients |
| topic | GeneXpert MTB/RIF MTB Mycobacterium tuberculosis Nepal Smear-negative |
| url | https://doi.org/10.1186/s12879-022-07287-5 |
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