Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies
Background Exercise elicits a range of adaptive responses in skeletal muscle, which include changes in mRNA expression. To better understand the health benefits of exercise training, it is important to investigate the underlying molecular mechanisms of skeletal muscle adaptation to exercise. However...
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PeerJ Inc.
2022-02-01
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| Online Access: | https://peerj.com/articles/12856.pdf |
| _version_ | 1797419215367438336 |
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| author | Jujiao Kuang Cian McGinley Matthew J-C Lee Nicholas J. Saner Andrew Garnham David J. Bishop |
| author_facet | Jujiao Kuang Cian McGinley Matthew J-C Lee Nicholas J. Saner Andrew Garnham David J. Bishop |
| author_sort | Jujiao Kuang |
| collection | DOAJ |
| description | Background Exercise elicits a range of adaptive responses in skeletal muscle, which include changes in mRNA expression. To better understand the health benefits of exercise training, it is important to investigate the underlying molecular mechanisms of skeletal muscle adaptation to exercise. However, most studies have assessed the molecular events at only a few time-points within a short time frame post-exercise, and the variations of gene expression kinetics have not been addressed systematically. Methods We assessed the mRNA expression of 23 gene isoforms implicated in the adaptive response to exercise at six time-points (0, 3, 9, 24, 48, and 72 h post exercise) over a 3-day period following a single session of high-intensity interval exercise. Results The temporal patterns of target gene expression were highly variable and the expression of mRNA transcripts detected was largely dependent on the timing of muscle sampling. The largest fold change in mRNA expression of each tested target gene was observed between 3 and 72 h post-exercise. Discussion and Conclusions Our findings highlight an important gap in knowledge regarding the molecular response to exercise, where the use of limited time-points within a short period post-exercise has led to an incomplete understanding of the molecular response to exercise. Muscle sampling timing for individual studies needs to be carefully chosen based on existing literature and preliminary analysis of the molecular targets of interest. We propose that a comprehensive time-course analysis on the exercise-induced transcriptional response in humans will significantly benefit the field of exercise molecular biology. |
| first_indexed | 2024-03-09T06:45:09Z |
| format | Article |
| id | doaj.art-457fa5b2fffd49bab5cf527bcd703d27 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:45:09Z |
| publishDate | 2022-02-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-457fa5b2fffd49bab5cf527bcd703d272023-12-03T10:38:00ZengPeerJ Inc.PeerJ2167-83592022-02-0110e1285610.7717/peerj.12856Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsiesJujiao Kuang0Cian McGinley1Matthew J-C Lee2Nicholas J. Saner3Andrew Garnham4David J. Bishop5Institute for Health and Sport, Victoria University, Melbourne, Victoria, AustraliaSportscotland Institute of Sport, Stirling, United KingdomInstitute for Health and Sport, Victoria University, Melbourne, Victoria, AustraliaInstitute for Health and Sport, Victoria University, Melbourne, Victoria, AustraliaInstitute for Health and Sport, Victoria University, Melbourne, Victoria, AustraliaInstitute for Health and Sport, Victoria University, Melbourne, Victoria, AustraliaBackground Exercise elicits a range of adaptive responses in skeletal muscle, which include changes in mRNA expression. To better understand the health benefits of exercise training, it is important to investigate the underlying molecular mechanisms of skeletal muscle adaptation to exercise. However, most studies have assessed the molecular events at only a few time-points within a short time frame post-exercise, and the variations of gene expression kinetics have not been addressed systematically. Methods We assessed the mRNA expression of 23 gene isoforms implicated in the adaptive response to exercise at six time-points (0, 3, 9, 24, 48, and 72 h post exercise) over a 3-day period following a single session of high-intensity interval exercise. Results The temporal patterns of target gene expression were highly variable and the expression of mRNA transcripts detected was largely dependent on the timing of muscle sampling. The largest fold change in mRNA expression of each tested target gene was observed between 3 and 72 h post-exercise. Discussion and Conclusions Our findings highlight an important gap in knowledge regarding the molecular response to exercise, where the use of limited time-points within a short period post-exercise has led to an incomplete understanding of the molecular response to exercise. Muscle sampling timing for individual studies needs to be carefully chosen based on existing literature and preliminary analysis of the molecular targets of interest. We propose that a comprehensive time-course analysis on the exercise-induced transcriptional response in humans will significantly benefit the field of exercise molecular biology.https://peerj.com/articles/12856.pdfExerciseExercise-induced adaptationGene expressionSkeletal muscleTime-course |
| spellingShingle | Jujiao Kuang Cian McGinley Matthew J-C Lee Nicholas J. Saner Andrew Garnham David J. Bishop Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies PeerJ Exercise Exercise-induced adaptation Gene expression Skeletal muscle Time-course |
| title | Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies |
| title_full | Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies |
| title_fullStr | Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies |
| title_full_unstemmed | Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies |
| title_short | Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies |
| title_sort | interpretation of exercise induced changes in human skeletal muscle mrna expression depends on the timing of the post exercise biopsies |
| topic | Exercise Exercise-induced adaptation Gene expression Skeletal muscle Time-course |
| url | https://peerj.com/articles/12856.pdf |
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