Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90%

Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm. The proposed method seeks to reduce the presence of cryoprot...

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Bibliographic Details
Main Authors: Maritza Pérez-Atehortúa, Andrea Galuppo, Rômulo Batista Rodrigues, Nathalia dos Santos Teixeira, Thaiza Rodrigues de Freitas, Thales de Souza França, Lis Santos Marques, Itamar Cossina Gomes, Danilo Pedro Streit, Jr.
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121002156