Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600008 |
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| author | Erika Noda Noguti Clarice Queico Fujimura Leite Ana Carolina Malaspina Adolfo Carlos Barreto Santos Rosário Dominguez Crespo Hirata Mario Hiroyuki Hirata Elsa Massae Mamizuka Rosilene Fressatti Cardoso |
| author_facet | Erika Noda Noguti Clarice Queico Fujimura Leite Ana Carolina Malaspina Adolfo Carlos Barreto Santos Rosário Dominguez Crespo Hirata Mario Hiroyuki Hirata Elsa Massae Mamizuka Rosilene Fressatti Cardoso |
| author_sort | Erika Noda Noguti |
| collection | DOAJ |
| description | The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating. |
| first_indexed | 2024-03-12T18:04:18Z |
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| id | doaj.art-45873b408a44426aaa5d7f474c96635a |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:04:18Z |
| publishDate | 2010-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-45873b408a44426aaa5d7f474c96635a2023-08-02T09:32:14ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-09-01105677978510.1590/S0074-02762010000600008Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern BrazilErika Noda NogutiClarice Queico Fujimura LeiteAna Carolina MalaspinaAdolfo Carlos Barreto SantosRosário Dominguez Crespo HirataMario Hiroyuki HirataElsa Massae MamizukaRosilene Fressatti CardosoThe purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600008Mycobacterium tuberculosistuberculosismolecular epidemiologyspoligotypingMIRU |
| spellingShingle | Erika Noda Noguti Clarice Queico Fujimura Leite Ana Carolina Malaspina Adolfo Carlos Barreto Santos Rosário Dominguez Crespo Hirata Mario Hiroyuki Hirata Elsa Massae Mamizuka Rosilene Fressatti Cardoso Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil Memorias do Instituto Oswaldo Cruz Mycobacterium tuberculosis tuberculosis molecular epidemiology spoligotyping MIRU |
| title | Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
| title_full | Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
| title_fullStr | Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
| title_full_unstemmed | Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
| title_short | Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
| title_sort | genotyping of mycobacterium tuberculosis isolates from alow endemic setting in northwestern state of parana in southern brazil |
| topic | Mycobacterium tuberculosis tuberculosis molecular epidemiology spoligotyping MIRU |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600008 |
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