Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses
We applied femtosecond laser pulses for microsurgery of the zona pellucida (ZP) of mouse embryos in terms of assisted reproductive technologies. The embryos were subjected to a series of laser pulses (wavelength of 514 nm, pulse duration of 280 fs, repetition rate of 2.5 kHz). Optical microscopy was...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2076-3417/13/20/11204 |
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| author | Dmitry S. Sitnikov Maxim A. Filatov Inna V. Ilina |
| author_facet | Dmitry S. Sitnikov Maxim A. Filatov Inna V. Ilina |
| author_sort | Dmitry S. Sitnikov |
| collection | DOAJ |
| description | We applied femtosecond laser pulses for microsurgery of the zona pellucida (ZP) of mouse embryos in terms of assisted reproductive technologies. The embryos were subjected to a series of laser pulses (wavelength of 514 nm, pulse duration of 280 fs, repetition rate of 2.5 kHz). Optical microscopy was used to study the dependence of the ZP cut width on the pulse energy <i>E</i> and velocity <i>v</i> of the laser beam. It is shown that the same value of the cut width can be obtained for different combinations of these parameters. The boundaries of admissible values were found to be <i>E</i> = (19–52) nJ, <i>v</i> = (0.001–0.03) mm/s; recommendations on their proper choice are given. An analytical expression of the cut width of the ZP for a given combination of laser-pulse energy, beam velocity, and pulse repetition rate is proposed. This simple and easy-to-use equation allows for quick prediction of ZP cut width in embryo microsurgery procedures of laser-assisted hatching and embryo laser tagging using femtosecond laser pulses. |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T21:28:16Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-458a7024eba143b0ac82a21ba67a5bbc2023-11-19T15:30:04ZengMDPI AGApplied Sciences2076-34172023-10-0113201120410.3390/app132011204Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser PulsesDmitry S. Sitnikov0Maxim A. Filatov1Inna V. Ilina2Joint Institute for High Temperatures of the Russian Academy of Sciences, Izhorskaya Str., 13, Bldg. 2, Lab. 1.2, JIHT RAS, Moscow 125412, RussiaJoint Institute for High Temperatures of the Russian Academy of Sciences, Izhorskaya Str., 13, Bldg. 2, Lab. 1.2, JIHT RAS, Moscow 125412, RussiaJoint Institute for High Temperatures of the Russian Academy of Sciences, Izhorskaya Str., 13, Bldg. 2, Lab. 1.2, JIHT RAS, Moscow 125412, RussiaWe applied femtosecond laser pulses for microsurgery of the zona pellucida (ZP) of mouse embryos in terms of assisted reproductive technologies. The embryos were subjected to a series of laser pulses (wavelength of 514 nm, pulse duration of 280 fs, repetition rate of 2.5 kHz). Optical microscopy was used to study the dependence of the ZP cut width on the pulse energy <i>E</i> and velocity <i>v</i> of the laser beam. It is shown that the same value of the cut width can be obtained for different combinations of these parameters. The boundaries of admissible values were found to be <i>E</i> = (19–52) nJ, <i>v</i> = (0.001–0.03) mm/s; recommendations on their proper choice are given. An analytical expression of the cut width of the ZP for a given combination of laser-pulse energy, beam velocity, and pulse repetition rate is proposed. This simple and easy-to-use equation allows for quick prediction of ZP cut width in embryo microsurgery procedures of laser-assisted hatching and embryo laser tagging using femtosecond laser pulses.https://www.mdpi.com/2076-3417/13/20/11204femtosecond laser pulsesmicrosurgeryembryozona pellucidalaser-assisted hatchingassisted reproductive technologies |
| spellingShingle | Dmitry S. Sitnikov Maxim A. Filatov Inna V. Ilina Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses Applied Sciences femtosecond laser pulses microsurgery embryo zona pellucida laser-assisted hatching assisted reproductive technologies |
| title | Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses |
| title_full | Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses |
| title_fullStr | Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses |
| title_full_unstemmed | Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses |
| title_short | Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses |
| title_sort | optimal exposure parameters for microsurgery of embryo zona pellucida using femtosecond laser pulses |
| topic | femtosecond laser pulses microsurgery embryo zona pellucida laser-assisted hatching assisted reproductive technologies |
| url | https://www.mdpi.com/2076-3417/13/20/11204 |
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