Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering
Objective: to investigate the efficacy of supercritical carbon dioxide (sc-CO2) for enhancштп the biocompatibility of biopolymer scaffolds from biodegradable materials and tissue-specific scaffolds from decellularized porcine liver slices (PLSs) or fine porcine cartilage particles (FPCPs).Materials...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2021-07-01
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| Series: | Vestnik Transplantologii i Iskusstvennyh Organov |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/1301 |
| _version_ | 1797871922574262272 |
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| author | E. A. Nemets A. E. Lazhko A. M. Grigoryev V. Yu. Belov V. A. Surguchenko Yu. B. Basok A. D. Kirillova V. I. Sevastianov |
| author_facet | E. A. Nemets A. E. Lazhko A. M. Grigoryev V. Yu. Belov V. A. Surguchenko Yu. B. Basok A. D. Kirillova V. I. Sevastianov |
| author_sort | E. A. Nemets |
| collection | DOAJ |
| description | Objective: to investigate the efficacy of supercritical carbon dioxide (sc-CO2) for enhancштп the biocompatibility of biopolymer scaffolds from biodegradable materials and tissue-specific scaffolds from decellularized porcine liver slices (PLSs) or fine porcine cartilage particles (FPCPs).Materials and methods. Biopolymer scaffolds of a polyoxy(butyrate-co-valerate) and gelatin copolymer composition, 4 mm in diameter and 80 mm in length, were formed by electrospinning (NANON-01A, MECC CO, Japan) and stabilized by incubation in glutaraldehyde vapor for 48 hours at room temperature. For decellularization, PLSs and FPCPs were incubated under periodic stirring in buffer (pH = 7.4) solutions of sodium dodecyl sulfate (0.1%) and Triton X-100 with increasing concentrations (1, 2, and 3%). Treatment in a sc-CO2 atmosphere was done at 150–300 bar pressure, 35 °C temperature, and 0.25–2.5 mL/min flow rate of sc-CO2 for 8–24 hours. 10% ethanol was introduced as a polarity modifier. Cytotoxicity was studied according to GOST ISO 10993-5-2011. The growth of NIH/3T3 in the presence of samples was studied using an interactive optical system IncuCyte Zoom.Results. The effect of the sc-CO2 flow rate and pressure, and the effect of addition of ethanol, on the biocompatibility of scaffolds was investigated. It was found that treatment at a low sc-CO2 flow rate (0.25 mL/min) does not achieve the required cytotoxicity. Complete absence of cytotoxicity in biopolymer scaffolds was achieved in the presence of 10% ethanol, at a sc-CO2 flow rate of 2.5 mL/min, 300 bar pressure and 35 °C temperature after 8 hours of treatment. Effective removal of cytotoxic detergents from decellularized liver occurs already at a 150-bar pressure and does not require the addition of ethanol. Adding ethanol to sc-CO2 eliminates not only the cytotoxic, but also the cytostatic effect of tissue-specific scaffolds.Conclusion. Sc-CO2 treatment is an effective way to enhance the biocompatibility of three-dimensional porous matrices produced using cytotoxic substances: bifunctional crosslinking agents for biopolymer scaffolds and surfactants in the case of tissue-specific matrices. Addition of ethanol as a polarity modifier improves the treatment efficiency by eliminating both cytotoxic and cytostatic effects. |
| first_indexed | 2024-04-10T00:51:49Z |
| format | Article |
| id | doaj.art-458ab095dcf449d0b959c1a30e1dfaec |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2024-04-10T00:51:49Z |
| publishDate | 2021-07-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Vestnik Transplantologii i Iskusstvennyh Organov |
| spelling | doaj.art-458ab095dcf449d0b959c1a30e1dfaec2023-03-13T10:37:27ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovVestnik Transplantologii i Iskusstvennyh Organov1995-11912021-07-0123210411310.15825/1995-1191-2021-2-104-113976Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineeringE. A. Nemets0A. E. Lazhko1A. M. Grigoryev2V. Yu. Belov3V. A. Surguchenko4Yu. B. Basok5A. D. Kirillova6V. I. Sevastianov7Shumakov National Medical Research Center of Transplantology and Artificial Organs; Institute for Biomedical Research and TechnologyKurnakov Institute of General and Inorganic ChemistryShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial Organs; Institute for Biomedical Research and TechnologyShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial Organs; Institute for Biomedical Research and TechnologyShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial Organs; Institute for Biomedical Research and TechnologyObjective: to investigate the efficacy of supercritical carbon dioxide (sc-CO2) for enhancштп the biocompatibility of biopolymer scaffolds from biodegradable materials and tissue-specific scaffolds from decellularized porcine liver slices (PLSs) or fine porcine cartilage particles (FPCPs).Materials and methods. Biopolymer scaffolds of a polyoxy(butyrate-co-valerate) and gelatin copolymer composition, 4 mm in diameter and 80 mm in length, were formed by electrospinning (NANON-01A, MECC CO, Japan) and stabilized by incubation in glutaraldehyde vapor for 48 hours at room temperature. For decellularization, PLSs and FPCPs were incubated under periodic stirring in buffer (pH = 7.4) solutions of sodium dodecyl sulfate (0.1%) and Triton X-100 with increasing concentrations (1, 2, and 3%). Treatment in a sc-CO2 atmosphere was done at 150–300 bar pressure, 35 °C temperature, and 0.25–2.5 mL/min flow rate of sc-CO2 for 8–24 hours. 10% ethanol was introduced as a polarity modifier. Cytotoxicity was studied according to GOST ISO 10993-5-2011. The growth of NIH/3T3 in the presence of samples was studied using an interactive optical system IncuCyte Zoom.Results. The effect of the sc-CO2 flow rate and pressure, and the effect of addition of ethanol, on the biocompatibility of scaffolds was investigated. It was found that treatment at a low sc-CO2 flow rate (0.25 mL/min) does not achieve the required cytotoxicity. Complete absence of cytotoxicity in biopolymer scaffolds was achieved in the presence of 10% ethanol, at a sc-CO2 flow rate of 2.5 mL/min, 300 bar pressure and 35 °C temperature after 8 hours of treatment. Effective removal of cytotoxic detergents from decellularized liver occurs already at a 150-bar pressure and does not require the addition of ethanol. Adding ethanol to sc-CO2 eliminates not only the cytotoxic, but also the cytostatic effect of tissue-specific scaffolds.Conclusion. Sc-CO2 treatment is an effective way to enhance the biocompatibility of three-dimensional porous matrices produced using cytotoxic substances: bifunctional crosslinking agents for biopolymer scaffolds and surfactants in the case of tissue-specific matrices. Addition of ethanol as a polarity modifier improves the treatment efficiency by eliminating both cytotoxic and cytostatic effects.https://journal.transpl.ru/vtio/article/view/1301pig liverpig cartilagedecellularizationbiopolymer scaffoldssupercritical co2polarity modifiercytotoxicitybiocompatibility |
| spellingShingle | E. A. Nemets A. E. Lazhko A. M. Grigoryev V. Yu. Belov V. A. Surguchenko Yu. B. Basok A. D. Kirillova V. I. Sevastianov Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering Vestnik Transplantologii i Iskusstvennyh Organov pig liver pig cartilage decellularization biopolymer scaffolds supercritical co2 polarity modifier cytotoxicity biocompatibility |
| title | Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering |
| title_full | Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering |
| title_fullStr | Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering |
| title_full_unstemmed | Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering |
| title_short | Supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering |
| title_sort | supercritical carbon dioxide as a tool for improving the biocompatible properties of biopolymer and tissue specific scaffolds for tissue engineering |
| topic | pig liver pig cartilage decellularization biopolymer scaffolds supercritical co2 polarity modifier cytotoxicity biocompatibility |
| url | https://journal.transpl.ru/vtio/article/view/1301 |
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